Restriction endonuclease mapping by crossed contact hybridization: the ribosomal RNA genes of Achlya ambisexuahis

Rozek, Charles E.; Timberlake, W E

doi:10.1093/nar/7.6.1567

Cited by 38 publications

(6 citation statements)

References 28 publications

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“…Total cellular RNA was prepared according to Rozek and Davidson (1983). Poly(A +) fractions were selected by oligo dT cellulose (Collaborative Research, Inc., Bedford, Massachusetts, Type T3) chromatography according to Rozek et al (1978). Sample preparation and electrophoresis for RNA blot analysis was carried out according to Rozek and Davidson (1983).…”

Section: Isolation Of D Virilis Cyt-b Gene and Clone Preparations A Dmentioning

confidence: 99%

Evolutionary divergence of the cytochrome b5 gene of Drosophila

1995

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Cytochrome proteins perform a broad spectrum of biological functions ranging from oxidative metabolism to electron transport and are thus essential to all organisms. The b-type cytochrome proteins bind heme noncovalently, are expressed in many different forms and are localized to various cellular compartments. We report the characterization of the cytochrome b5 (Cyt-b) gene of Drosophila virilis and compare its structure to the Cyt-b gene of Drosophila melanogaster. As in D. melanogaster, the D. virilis gene is nuclear encoded and single copy. Although the intron/exon structures of these homologues differ, the Cyt-b proteins of D. melanogaster and D. virilis are approximately 75% identical and share the same size coding regions (1,242 nucleotides) and protein products (414 amino acids). The Drosophila Cyt-b proteins show sequence similarity to other b-type cytochromes, especially in the N-terminal heme-binding domain, and may be targeted to the mitochondrial membrane. The greatest levels of similarity are observed in areas of potential importance for protein structure and function. The exon sequences of the D. virilis Cyt-b gene differ by a total of 292 base changes. However, 62% of these changes are silent. The high degree of conservation between species separated by 60 million years of evolution in both the DNA and amino acid sequences suggests this nuclear cytochrome b5 locus encodes an essential product of the Drosophila system.

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Section: Isolation Of D Virilis Cyt-b Gene and Clone Preparations A Dmentioning

confidence: 99%

Evolutionary divergence of the cytochrome b5 gene of Drosophila

1995

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“…contact procedure of Rozek & Timberlake (40). For this 300pg of pCNZ (1OOg/ml) DNA, which had been treated with lOng/ml DNase 1 for 10 min at 37°C and then heated to 100.C for 10 min, was cast in a 15cm x 15cm horizontal 1% agarose gel.…”

Section: Nucleic Acids Researchmentioning

confidence: 99%

Isolation of a fraction from cauliflower mosaic virus-infected protoplasts which is active in the synthesis of (+) and (−) strand viral DNA and reverse transcription of primed RNA templates

et al. 1985

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Sub-cellular fractions, isolated from cauliflower mosaic virus (CaMV)-infected turnip protoplasts, are capable of synthesising CaMV DNA in vitro on an endogenous template and of reverse transcribing oligo dT-primed cowpea mosaic virus RNA. The activity was not detected in mock-inoculated protoplasts. In vitro-labelled DNA hybridized to single-stranded M13 clones complementary to the putative origins of (-) and (+) strand CaMV DNA synthesis and to restriction endonuclease fragments encompassing more than 90% of the CaMV genome. The synthesis of (-) and (+) strand DNA appeared asymmetric. The template(s) for in vitro CaMV DNA synthesis are in a partially nuclease-resistant form. Fractions capable of in vitro CaMV DNA synthesis contained CaMV RNA both heterogeneous and as discrete species; they also contained a range of different sizes of CaMV DNA. Several lines of evidence indicate that this range of in vitro-labelled CaMV DNA, extending from 0.6kb to 8.0kb in length, represents elongating (-) strand DNA. These are discussed in relation to their role as possible replicative intermediates.

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“…Some of this may be attrib uted to secondary introductions of unlinked 5s into 18S-5.8S25S rDNA repeating units (Drouin et al 1987, 1992, Drouin and Moniz de Sa 1995 The division Chromophyta (Christensen 1962), or the kingdom Chromista (Cavalier-Smith 1986, is a large heterogeneous group that includes more than 10 class-level or higher lineages. To date, rRNA gene organization has only been investigated in two of the lineages, the Phaeophyceae (Kawai et al 1995) and oomycetes (Rozek and Timberlake 1979). The present paper surveys rDNA organization in representative members of the remaining lineages in order to explore the phylogenetic implications of these data.…”

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LINKAGE OF 5S RIBOSOMAL DNA TO OTHER rDNAS IN THE CHROMOPHYTIC ALGAE AND RELATED TAXA¹

Kawai

Nakayama

Inouye

et al. 1997

Journal of Phycology

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Gene organization within the nuclear ribosomal DNA cistron and linkage of the 5s rDNA gene to the cistron were suruqed in 20 taxa of protists representing most of the Chromophyta (stramenopiles) and representatives of the Dinophyceae (alveolata) and Euglenophy ceae. The intergenic spacer, which separates adjacent cistrons, was Jirst PCR-ampl$ed from total DNA using primers anchored in the 3' end of the large subunit and the 5' end ofthe small subunit in the next downstream cistron. Presence of the 5s gene in the cistron was determined 4 a second round of PCR using primers anchored in the large subunit and the 5s gene. where 5S-linked rDNA was not detected in the cistron, the presence of 5s tandem repeating units were confirmed by the PCR of 5S5S f r a p n t s from the total DNA. Results show that most ofthe Chromophyta, as well as Opalina, Proteromonas (colorless stramenopiles), Dinophyceae, and Euglenophyceae have a 5s-linked Qpe of rDNA organization. In contrast, only tandem repeats of 5s rDNA were detected in Bacillariophy ceae and Synurophyceae. The occurrence of 5Sunlinked rDNA is hypothesized to be the result of seconda? transfer from an ancestral, linked 5s type. The 5s-linked type of rDNA organization is apparently common in protists. Given the fact that most of these protists have mitochondria with tubular or discoid cristae, as compared with flattened cristae common in higher plants and animals, we conclude that the 5Slinked type of rDNA diverged at a very ear4 stage in the evolution of eukaryotes.

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Restriction endonuclease mapping by crossed contact hybridization: the ribosomal RNA genes of Achlya ambisexuahis

Cited by 38 publications

References 28 publications

Evolutionary divergence of the cytochrome b5 gene of Drosophila

Evolutionary divergence of the cytochrome b5 gene of Drosophila

Isolation of a fraction from cauliflower mosaic virus-infected protoplasts which is active in the synthesis of (+) and (−) strand viral DNA and reverse transcription of primed RNA templates

LINKAGE OF 5S RIBOSOMAL DNA TO OTHER rDNAS IN THE CHROMOPHYTIC ALGAE AND RELATED TAXA¹

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Restriction endonuclease mapping by crossed contact hybridization: the ribosomal RNA genes of Achlya ambisexuahis

Cited by 38 publications

References 28 publications

Evolutionary divergence of the cytochrome b5 gene of Drosophila

Evolutionary divergence of the cytochrome b5 gene of Drosophila

Isolation of a fraction from cauliflower mosaic virus-infected protoplasts which is active in the synthesis of (+) and (−) strand viral DNA and reverse transcription of primed RNA templates

LINKAGE OF 5S RIBOSOMAL DNA TO OTHER rDNAS IN THE CHROMOPHYTIC ALGAE AND RELATED TAXA1

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LINKAGE OF 5S RIBOSOMAL DNA TO OTHER rDNAS IN THE CHROMOPHYTIC ALGAE AND RELATED TAXA¹