Restriction Endonuclease Analysis of Pasteurella multocida Isolates from Three California Turkey Premises

Kh, Christiansen; Te, Carpenter; Kp, Snipes; Dw, Hird; Gy, Ghazikhanian

doi:10.2307/1591501

Cited by 16 publications

(16 citation statements)

References 10 publications

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“…There have been many studies on the epidemiology of fowl cholera outbreaks in a variety of hosts; for example, in turkeys (Snipes et al, 1989(Snipes et al, , 1990Christiansen et al, 1992;Blackall et al, 1995;Kardos & Kiss, 2005), geese (Kardos & Kiss, 2005), free-range layers (Zhang et al, 2004), conventional layer flocks (Shivachandra et al, 2005;Eigaard et al, 2006), backyard poultry (Christensen et al, 1998) and ducks (Muhairwa et al, 2000). Unlike these prior studies, our current study has used both a phenotypic method (Heddleston serotyping) as well as a genotypic method (MLST).…”

Section: Discussionmentioning

confidence: 99%

“…In some studies, there have been multiple genotypes associated with outbreaks in turkeys (Snipes et al, 1989(Snipes et al, , 1990Christiansen et al, 1992). In contrast, other outbreaks have been reported as being associated with a single clone; for example, in turkey flocks (Blackall et al, 1995), in free-range layers (Zhang et al, 2004), in conventional layer flocks (Shivachandra et al, 2005;Eigaard et al, 2006) and in backyard poultry (Christensen et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

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Studies on the presence and persistence ofPasteurella multocidaserovars and genotypes in fowl cholera outbreaks

Singh

Blackall

Remington³

2013

Avian Pathology

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Pasteurella multocida was isolated from poultry on six farms (one free-range duck farm, one free-range turkey farm, one conventional enclosed turkey farm, and three free-range layer farms) suffering fowl cholera outbreaks. In addition, historical isolates from previous outbreaks were available for the conventional turkey farm and the three free-range layer farms. The isolates were serotyped using the Heddleston scheme and genotyped using multi-locus sequencing typing. In the current outbreaks, two of the farms had two different sequence types (STs) of P. multocida in the investigated outbreak (the free-range turkey farm and one of the free-range layer farms). The remaining four farms had one ST within the investigated outbreak. In looking at the historical isolates, two of the four farms had multiple genotypes involved. On the four farms with historical isolates from previous outbreaks, at least one new genotype was present in the investigated outbreak as compared with the historical isolates. On one layer farm, one genotype persisted over a 10-year period. Serotyping revealed the presence of multiple serovars in the current and historical outbreaks, with serovars sometimes changing over time. This study has shown that several STs of P. multocida can be present during some outbreaks of fowl cholera, although other outbreaks involve a single ST. Also, the STs present on a property suffering repeated fowl cholera can both persist and change over time.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Studies on the presence and persistence ofPasteurella multocidaserovars and genotypes in fowl cholera outbreaks

Singh

Blackall

Remington³

2013

Avian Pathology

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“…1,5 The finding of a single clone within a property is not universal as 1 previous study has shown the presence of multiple REA types within 1 of the 3 turkey farms studied. 5 The only previous molecular study of fowl cholera outbreaks with time involved a Muscovy duck farm. 13 This farm was a multiage farm with a yearly cleanup period in which no bird was present on the property.…”

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confidence: 90%

“…15 Molecular techniques such as restriction endonuclease analysis (REA) and ribotyping have enabled researchers to distinguish clones of P multocida as a method of studying outbreaks of fowl cholera in turkeys. 1,2,4,5,11 However, there are only lim-ited reports of the use of these techniques for fowl cholera outbreaks associated with chickens. 3,13,14 In this report, the use of REA and conventional biochemical and serological typing to examine the molecular epidemiology of 2 acute outbreaks of fowl cholera, separated by 8 years, on a free-range layer farm is described.…”

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confidence: 99%

Molecular Epidemiology of Two Fowl Cholera Outbreaks on a Free-Range Chicken Layer Farm

et al. 2004

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Two outbreaks of fowl cholera on a multiage free-range egg farm were investigated. The outbreaks occurred in 1994 and 2002. A total of 22 strains of Pasteurella multocida were available for study, 11 from the 1994 outbreak and 11 from the 2002 outbreak. Lesions typical of acute fowl cholera were seen in the 1994 outbreak, whereas both acute and chronic fowl cholera occurred in the 2002 outbreak. The isolates were examined in an extended phenotypic typing methodology, by a P. multocida–specific polymerase chain reaction (PCR), by the Heddleston somatic serotyping scheme, and by restriction endonuclease analysis (REA) typing using the enzyme HpaII. All 22 strains had the same phenotypic properties, all were confirmed as P. multocida by PCR, all were Heddleston serovar 4, and all had the same REA pattern. The results indicate that these 2 outbreaks were caused by the same clone of P. multocida–-despite the 8-year time period between the outbreaks.

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“…multocida has been found worldwide in association with various disease conditions in a broad range of hosts, including mammals and both domesticated and wild birds (Frank, 1989;Glisson et al ., 2003). Previous epidemiological investigations have demonstrated that P. multocida associated with fowl cholera in single domestic turkey flocks may belong to multiple clones (Snipes et al ., 1989(Snipes et al ., , 1990Christiansen et al ., 1992;Rhoades et al ., 1992), indicating the importance of characterizing multiple isolates from fowl cholera outbreaks to obtain reliable epidemiological information. The association of a single clone with outbreaks of fowl cholera has been reported in turkey (Christiansen et al ., 1992;Blackall et al ., 1995) and layer flocks (Zhang et al ., 2004), but this was based on relatively few isolates.…”

Section: Introductionmentioning

confidence: 99%

Clonal stability ofPasteurella multocidain free-range layers affected by fowl cholera

et al. 2006

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Detailed longitudinal studies of the genetic stability of Pasteurella multocida ssp. multocida, the cause of fowl cholera, have not previously been carried out. Consequently, the aim of the present study was to provide detailed information on the genetic stability and diversity of P. multocida ssp. multocida in poultry flocks over time, enabling new insights into the molecular epidemiology of this important poultry pathogen. Longitudinal investigations of the rate and causes of mortality were carried out on two free-range layer farms (A and B) over a period of 11 months. The total mortality of two flocks, A 1 and A 2 , on farm A were 62 and 91%, respectively, while the total mortality of a single flock B 1 on farm B was 6%. Postmortem examinations were performed on 708 layers from flocks A 1 and A 2 and in 159 from flock B 1 . Fowl cholera was the main cause of mortality on both farms. Pasteurella multocida isolates recovered from layers on both farms were characterized phenotypically and genotypically, and 322 isolates were identified as P. multocida ssp. multocida. The genetic diversity of 99 isolates from farm A and 31 from farm B was characterized by restriction endonuclease analysis and amplified fragment length polymorphism analysis. The isolates on each farm had a unique restriction endonuclease analysis and amplified fragment length polymorphism analysis type, suggesting a

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Restriction Endonuclease Analysis of Pasteurella multocida Isolates from Three California Turkey Premises

Cited by 16 publications

References 10 publications

Studies on the presence and persistence ofPasteurella multocidaserovars and genotypes in fowl cholera outbreaks

Studies on the presence and persistence ofPasteurella multocidaserovars and genotypes in fowl cholera outbreaks

Molecular Epidemiology of Two Fowl Cholera Outbreaks on a Free-Range Chicken Layer Farm

Clonal stability ofPasteurella multocidain free-range layers affected by fowl cholera

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