Restriction Endonuclease Analysis of Mycoplasma synoviae Strains

Abstract

A diverse group of Mycoplasma synoviae strains from various hosts, pathological processes, and geographic areas collected over about 25 years were analyzed by restriction endonuclease analysis. The results suggest that restriction endonuclease analysis of M. synoviae strains may be a useful strain identification tool to study epidemiological problems.

Variable lipoprotein haemagglutinin (vlhA) gene sequence typing of mainly DutchMycoplasma synoviaeisolates: comparison withvlhAsequences from Genbank and with amplified fragment length polymorphism analysis

“…Several molecular techniques for typing M. synoviae isolates, amongst others pulsed-field gel electrophoresis (Morrow et al, 1990b;Ley & Avakian, 1992;Marois et al, 2001;Dufour-Gesbert et al, 2006;Harada et al, 2009) and random amplified polymorphic DNA analysis (Geary et al, 1994;Marois et al, 2001;Feberwee et al, 2005;DufourGesbert et al, 2006), have been described. However, the discriminatory power of these genomic methods in differentiation of field isolates is relatively low, as a considerable homology between unrelated isolates was sometimes observed (Dufour-Gesbert et al, 2006).…”

Classification of Mycoplasma synoviae strains using single-strand conformation polymorphism and high-resolution melting-curve analysis of the vlhA gene single-copy region

“…defining the strain involved) is very useful for epidemiological tracing and is of critical importance in countries in which poultry flocks are vaccinated with the live attenuated M. synoviae strain MS-H (Vaxsafe MS, Bioproperties Australia). RFLP has been described elsewhere (Morrow et al, 1990b) for classification of M. synoviae strains and is currently in use in our laboratory for classification of new field M. synoviae strains. However, RFLP is time consuming, since it requires isolation and culture of the organism and extraction of genomic DNA, and can be unreliable, particularly because of genomic rearrangements that may occur in progenies of a single M. synoviae isolate (Noormohammadi et al, 2000).…”

Genotypic heterogeneity among strains ofMycoplasma gallinarum

“…Although our study included only eight M. gallinarum strains it seems that restriction endonuclease DNA analysis may have a potential use for studying the epidemiology of this Mycoplasma species, as has also been reported for M. gallisepticum and M. synoviae (Kleven et al, 1988 a,b;Morrow et al, 1990).…”

“…It has been reported, that examination of DNA restriction fragments using agarose gel electrophoresis after treatment may distinguish between strains of certain Mycoplasma species (Razin et al, 1983 a, b). The technique has been also successfully applied to M. gallisepticum and M. synoviae which are known avian pathogens (Kleven et al, 1988 a, b;Morrow et al, 1990). Having a positive experience with the field M. gallisepticum strains we examined also our local field strains of M. gallinarum using DNA restriction fragment analysis.…”