Restriction endonuclease analysis of BHV‐1 and BHV‐5 strains isolated in argentina

Pidone, Claudio Luis; Galosi, Cecília Mônica; Echeverría, María Gabriela; Nosetto, Edgardo Omar; Etcheverrigaray, María Elisa

doi:10.1046/j.1439-0450.1999.00257.x

Cited by 17 publications

(15 citation statements)

References 6 publications

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“…However, it is not highly precise because BoHV-1 can also be responsible for neurological disease [33,34]. Furthermore, immunoassays using monoclonal antibodies [4,35,36], PCR followed by REA [37], nested PCR [13] and multiplex PCR [38-40] are also available.…”

Section: Introductionmentioning

confidence: 99%

Characterization of BoHV-5 field strains circulation and report of transient specific subtype of bovine herpesvirus 5 in Argentina

et al. 2011

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BackgroundBovine herpesvirus 5 (BoHV-5) is a member of the subfamily Alphaherpesvirinae responsible for meningo-encephalitis in young cattle. The first case of bovine meningo-encephalitis associated with a herpesvirus infection was reported in Australia. The current geographical distribution of BoHV-5 infection is mainly restricted to South America, especially Brazil and Argentina. Outbreaks of BoHV-5 are regularly observed in Argentina suggesting the circulation of the virus in the bovine population.ResultsSeventeen field strains of BoHV-5 isolated from 1984 to now were confirmed by differential PCR and subjected to restriction endonuclease analysis (REA). Viral DNA was cleaved with BstEII which allows the differentiation among subtypes a, b and non a, non b. According to the REA with BstEII, only one field strain showed a pattern similar to the Argentinean A663 strain (prototype of BoHV-5b). All other isolates showed a clear pattern similar to the Australian N569 strain (prototype of BoHV-5a) consistent with the subtypes observed in Brazil, the other South-American country where BoHV-5 is known to be prevalent. The genomic region of subtype b responsible for the distinct pattern was determined and amplified by PCR; specifically a point mutation was identified in glycoprotein B gene, on the BstEII restriction site, which generates the profile specific of BoHV-5b.ConclusionsThis is the first report of circulation of BoHV-5a in Argentina as the prevailing subtype. Therefore the circulation of BoHV-5b was restricted to a few years in Argentina, speculating that this subtype was not able to be maintained in the bovine population. The mutation in the gB gene is associated with the difference in the restriction patterns between subtypes "a" and "b".

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Section: Introductionmentioning

confidence: 99%

Characterization of BoHV-5 field strains circulation and report of transient specific subtype of bovine herpesvirus 5 in Argentina

et al. 2011

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“…BoHV-1.2b isolates are considered of moderate pathogenicity (Metzler et al 1985) and may cause vulvovaginitis or balanopostitis, but have not so far been linked to abortions (Miller 1991, Smith et al 1995. Regarding BoHV-5, these have been further subdivided in BoHV-5a, BoHV-5b and BoHV-5 "non-a, non-b", or "c" (Pidone et al 1999, D'Arce et al 2002.…”

Section: Introductionmentioning

confidence: 99%

Serum neutralization with different types and subtypes of bovine herpesvirus 1 and 5

Holz

Cibulski²,

Teixeira

et al. 2010

Pesq. Vet. Bras.

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The serum neutralization (SN) test is the gold standard method to measure neutralizing antibodies to bovine herpesviruses. However, in view of the further subdivisions of bovine herpesviruses in types/subtypes, defining which virus to use at challenge in SN tests may be difficult. In view of that, this study was carried out to re-evaluate (SN) sensitivity with different types/subtypes of bovine herpesviruses types 1 (BoHV-1) and 5 (BoHV-5) as challenge viruses. Bovine sera (n=810) were collected from two distinct geographic regions and tested by SN with three type 1 viruses (BoHV-1.1 strains "Los Angeles" and "EVI123/98"; BoHV-1.2a strain "SV265/96") and three type 5 viruses (BoHV-5a strain "EVI88/95"; BoHV-5b strain "A663" and BoHV-5c "ISO97/95"). SN tests were performed with a 1 hour incubation of the serum-virus mixtures at 37ºC against 100 TCID50 of each of the viruses. SN sensitivity varied greatly depending on the challenge virus used in the test. The highest sensitivity (327 positive/810 total sera tested; 40.37%) was attained when the positive results to the six viruses were added together. No association could be found between any particular type or subtype of virus and the sensitivity of the test. When positive results to each single strain were considered, SN sensitivity varied from 41.7% to 81.7%, depending on the virus and the geographic region of origin of the sera. Variation was detected even when challenge viruses belonged to the same subtype, where disagreement between positive results reached 41%. These results indicate that one hour incubation SN tests against single viruses, as performed here, may display a significantly low sensitivity (p=0.05); performing SN tests against a number of different viruses may increase considerably SN sensitivity. Furthermore, the choice of virus used for challenge is critical in SN tests. In addition, sera from different geographic regions may give rise to disagreeing results with different strains of BoHV-1 and BoHV-5. This might be particularly relevant for control programs and in international trade, were maximum sensitivity should be targeted. RESUMO.-[Soroneutralização com diferentes tipos e subtipos de herpesvírus bovinos 1e 5.] O teste de soroneutralização (SN) é o método padrão para a mensuração de anticorpos neutralizantes para herpesvírus bovinos. Entretanto, com as subdivisões propostas destes agentes em tipos e subtipos, a definição de qual amostra utilizar como virus de desafio à SN pode ser difícil. Em vista disso, este estudo foi realizado para re-avaliar a sensibilidade de testes de SN utilizando diferentes tipos e subtipos de herpesvírus bovinos tipos 1 (BoHV-1) e 5 (BoHV-5) como amostras de desafio. Soros bovinos (n=810) foram coletados de duas regiões geográficas distintas e testados frente a amostras do tipo 1 (BoHV-1.1:Pesq. Vet. Bras. 30 (7):515-522, julho 2010Carine Lidiane Holz et al. 516 amostras "Los Angeles" e "EVI123/98", BoHV-1.2a: amostra "SV265/96") e três amostras do tipo 5 (BoHV-5a: "EVI88/95"; BoHV-5b: "A663" e BoHV-5c "ISO...

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“…Ello dio lugar a cambios importantes en la distribución del ganado bovino, como la intensificación de la carga de ganado en campos de menor calidad agrícola y la aparición de sistemas de producción mixtos (búfalo-bovino) 5 . Desde el punto de vista epidemiológico es interesante conocer que los alpha herpesvirus circulan en estos rebaños mixtos, siendo nuestro país endémico para los tipos BoHV-1, BoHV-5 9 . En búsqueda de una mejor comprensión de la epidemiología del herpesvirus bovino en el país, 44 aislamientos de campos de diferentes regiones agrícolas fueron examinados, pudiendo observarse que, independientemente del subtipo, las cepas mostraron tropismo diferente por las distintas mucosas.…”

Section: Discussionunclassified

Herpesvirus bovino 1 (BoHV-1): actualización de las cepas circulantes en Argentina

Maidana

Marin

Destefano³

et al. 2018

Rev. vet.

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Resumen Maidana, S.S.; Marin, M.; Destefano, G.; Combessies, G.; Romera, S.A.: Herpesvirus bovino 1 (BoHV-1): actualización de las cepas circulantes en Argentina. Rev. vet. 29: 1, 52-56, 2018. BoHV-1 genera en el ganado bovino una amplia gama de manifestaciones clínicas, tales como la rinotraqueítis infecciosa bovina, abortos y vulvovaginitis pustular infecciosa, que dependen de la virulencia del virus y del estado inmunitario del hospedador. En este trabajo se caracterizaron en Argentina 44 aislamientos de BoHV-1 por el análisis de restricción con endonucleasas (REA). El ADN viral fue sometido al clivaje de la enzima HindIII, la cual permite la diferenciación entre los subtipos 1.1, 1.2a y 1.2b. De acuerdo al perfil REA de los aislamientos, 35 cepas (80%) mostraron un patrón similar a la cepa K22 (prototipo BoHV-1.2b), 7 cepas (16%) exhibieron un patrón similar a la cepa LA (prototipo BoHV-1.1) y sólo 2 cepas (4%) revelaron un patrón similar a la cepa ST (prototipo BoHV-1.2a). Los resultados demuestran una mayor prevalencia del subtipo 1.2b entre los aislamientos BoHV-1 argentinos. Además, se reporta por primera vez en Argentina, la circulación del subtipo BoHV-1.2a. Rev. vet. 29: 1, 52-56, 2018. BoHV-1 in cattle results in a wide range of clinical manifestations, such as infectious bovine rhinotracheitis, abortion and infectious pustular vulvovaginitis, depending on the virulence of the virus and the immune status of the host. Forty-four Argentinean BoHV1 isolates were characterized by restriction endonuclease analysis (REA). Viral DNA was cleaved with HindIII which allows the differentiation among subtypes 1.1, 1.2a and 1.2b. According to the REA, 35 field strains (80%) showed a pattern similar to K22 strain (BoHV-1.2b prototype), 7 field strains (16%) showed a clear pattern similar to LA strain (BoHV-1.1 prototype) and only 2 filed strains (4%) showed a clear pattern similar to ST strain (BoHV-1.2a prototype). The results demonstrated the presence of a marked prevalence of subtype 1.2b among Argentinean BoHV-1 isolates. We also report for the first time in Argentina the circulation of the subtype BoHV-1.2a.

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Restriction endonuclease analysis of BHV‐1 and BHV‐5 strains isolated in argentina

Cited by 17 publications

References 6 publications

Characterization of BoHV-5 field strains circulation and report of transient specific subtype of bovine herpesvirus 5 in Argentina

Characterization of BoHV-5 field strains circulation and report of transient specific subtype of bovine herpesvirus 5 in Argentina

Serum neutralization with different types and subtypes of bovine herpesvirus 1 and 5

Herpesvirus bovino 1 (BoHV-1): actualización de las cepas circulantes en Argentina

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