Restriction endonuclease analysis of a Spodoptera litura nuclear polyhedrosis virus (NPV) isolate

Das, Rakha H.; Prasad, Y.

doi:10.1080/15216549600201001

Cited by 8 publications

(3 citation statements)

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“…Seven NPV genomes have now been sequenced and EppoMNPV is the smallest of these. The sizes of a number of other lepidopteran NPVs have been determined by restriction fragment analysis (Chen et al , 1996 ; Cheng & Carner, 2000 ; Das & Prasad, 1996 ; Hu et al , 1998 ; Li et al , 1997 ; Richards et al , 1999 ; Sadler et al , 2000 ) and these are all larger than EppoMNPV. The EppoMNPV genome has a G+C content of 40·7%, which is similar to all the other sequenced baculovirus genomes except OpMNPV and LdMNPV (Table 1).…”

Section: Resultsmentioning

confidence: 99%

Whole genome analysis of the Epiphyas postvittana nucleopolyhedrovirus

Hyink

Dellow

Olsen

et al. 2002

Journal of General Virology

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Section: Resultsmentioning

confidence: 99%

Whole genome analysis of the Epiphyas postvittana nucleopolyhedrovirus

Hyink

Dellow

Olsen

et al. 2002

Journal of General Virology

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“…A number of SpltNPV isolates have been isolated in Japan (Takatsuka, Okuno, Nakai, & Kunimi, 2003) and one of them is being used as microbial insecticide (Hasumon Tenteki, Nippon Kayaku Group;Mitsuhashi 2009). Similarly, in Taiwan and India, SpltNPV has been developed as insecticide against S. litura in vegetables, peanut, cotton and rice (Das & Durga, 1996;Moscardi, 1999). There are no reports that SpltNPV has been used in Pakistan and the virus-based insecticides available in other parts of the world would be a possible option of controlling S. litura.…”

Section: Introductionmentioning

confidence: 99%

Biological and genetic characterization of a Pakistani isolate of Spodoptera litura nucleopolyhedrovirus

Ali¹,

Werf

Vlak

2017

Biocontrol Science and Technology

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Spodoptera litura is an emerging insect pest in a wide range of crops worldwide. The insect is difficult to control because of resistance development to synthetic insecticides and emerging resistance to Bacillus thuringiensis toxins. Therefore, there is a need to develop biological control agents, preferably from an indigenous source to avoid risks associated with the importation of exotic natural antagonists. A Pakistani isolate of S. litura nucleopolyhedrovirus (SpltNPV, Baculoviridae), SpltNPV-Pak-BNG, was obtained from the field and characterized biologically and genetically, and compared to a SpltNPV reference isolate, SpltNPV-G1, thought to be of Chinese origin. The dose-mortality response (LD 50 ) of SpltNPVPak-BNG was not significantly different from that of the reference isolate SpltNPV-G1, but the time-to-death (LT 50 ) was significantly shorter for SpltNPV-Pak-BNG than for SpltNPV-G1. DNA restriction enzyme profiling indicated that SpltNPV-Pak-BNG and SpltNPV-G1 are different viruses. Sequence analysis of 'ORF24', specific for SpltNPV (and S. littoralis NPV as ORF21), and the conserved baculovirus core genes polyhedrin, DNApol, pif-2 and lef-8 confirmed that this was indeed the case and that SpltNPV-Pak-BNG is a genuine SpltNPV variant, whereas the SpltNPV-G1 isolate we used is, in fact, a SpliNPV variant, renamed to SpliNPV-G1. The newly isolated SpltNPV-Pak-BNG has the potential for development as a biocontrol agent of S. litura in Pakistan.ARTICLE HISTORY

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“…Type B SpltNPV coincide with NPV identified widely in S. litura in Japan (Laviana et al, 2001;Lavina-Caoili et al, 2001;Pang et al, 2001;Takatsuka et al, 2003). Type C isolate was unique in its REN pattern as compared to any of the previously isolated NPVs in S. litura or S. littoralis larvae, including SINPV-A (Cherry and Summer, 1985), SpltNPV isolated in India (Das and Prasad, 1996) and…”

Section: Spodoptera Litura Nucleopolyhedrovirus (Spltnpv)mentioning

confidence: 65%

Diversity and biological activity of nucleopolyhedroviruses of the leafworm Spodoptera litura

Ali

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Spodoptera litura is an emerging insect pest in a wide range of crops worldwide. The insect is difficult to control because of resistance development to synthetic insecticides and emerging resistance to Bacillus thuringiensis toxins. Therefore there is a need to develop biological control agents, preferably from an indigenous source to avoid risks associated with importation of exotic natural antagonists. A Pakistani isolate of S. litura nucleopolyhedrovirus (SpltNPV, Baculoviridae), SpltNPV-Pak-BNG, was obtained from the field and characterized biologically and genetically, and compared to a SpltNPV reference isolate, SpltNPV-G1, thought to be of Chinese origin. The dose mortality response (LD50) of SpltNPV-Pak-BNG was not significantly different from that of the reference isolate SpltNPV-G1, but the time-to-death (LT50) was significantly shorter for SpltNPV-Pak-BNG than for SpltNPV-G1. DNA restriction enzyme profiling indicated that SpltNPV-G1 and SpltNPV-Pak-BNG are different viruses. Sequence analysis of 'ORF24', specific for SpltNPV (and S. littoralis NPV as ORF21), and the conserved baculovirus core genes polyhedrin, DNApol, pif-2 and lef-8 confirmed that this was indeed the case and that SpltNPV-Pak-BNG is a genuine SpltNPV variant whereas the SpltNPV-G1 isolate we used is in fact a SpliNPV variant, renamed to SpliNPV-G1. The newly isolated SpltNPV-Pak-BNG has potential for development as a biocontrol agent of S. litura in Pakistan.

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Restriction endonuclease analysis of a Spodoptera litura nuclear polyhedrosis virus (NPV) isolate

Cited by 8 publications

References 0 publications

Whole genome analysis of the Epiphyas postvittana nucleopolyhedrovirus

Whole genome analysis of the Epiphyas postvittana nucleopolyhedrovirus

Biological and genetic characterization of a Pakistani isolate of Spodoptera litura nucleopolyhedrovirus

Diversity and biological activity of nucleopolyhedroviruses of the leafworm Spodoptera litura

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