Restriction Digest of Plasmid DNA v2

Abstract

MATERIALS TEXT Equipment EquipmentElectrophoresis chamber Pipetman Reagents Reagents Liquid DNA aliquot of your plasmid of interest (see below for recommend amounts) Appropriate restriction enzyme (see manufacturer's instructions for proper ammount) Approrpriate restriction digest buffer (see manufacturer's instructions) Gel loading dye Electrophoresis buffer Pipet tips1 Select restriction enzymes to digest your plasmid.