Restriction analysis of the prototype strain of enteric adenovirus type 41 using exonuclease III

Scott-Taylor, Tim H.; Ahluwalia, Gurmukh S.; Hammond, Gregory

doi:10.1016/0166-0934(92)90166-b

Cited by 5 publications

(3 citation statements)

References 22 publications

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“…Even though the genetics and growth characteristics of adenovirus 41 in culture have not been elaborately studied, 33,35 we believe that vectors derived from this adenoviral serotype would greatly enhance gene delivery to the intestinal epithelium. For the reasons described above, we are interested in designing recombinant adenoviral vectors that are targeted to intestinal epithelial cells.…”

Section: Introductionmentioning

confidence: 99%

In vitro and in vivo asessment of adenovirus 41 as a vector for gene delivery to the intestine

et al. 1998

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In order to identify suitable adenoviral vectors for efficient tiated enterocytes, probable targets for oral gene delivery delivery of transgenic proteins and peptides to the intesbut rather resistant to adenovirus-mediated gene transfer, tine, the ability of adenovirus types 5 and 41 (an enter-28.4% of the population internalized the Ad 5 vector and otropic serotype) to bind to and enter undifferentiated and less than 10% bound the virus. Adenovirus 41 was differentiated enterocytes was assessed. FACS analysis efficiently internalized in differentiated enterocytes as showed no significant difference between the virions in 89.6% of the cellular population took up the virus while their ability to bind to undifferentiated Caco-2 cells as 37.4% bound the virus. These results were consistent with 81.6% of the cellular population bound adenovirus 5 (Ad 5) those observed in vivo in rat jejunum. Thus, molecularly and 79.8% bound Ad 41. Both virions were also efficiently engineered Ad 41-based recombinants could be highly internalized in this cell type as 99.6% of the cells took up efficient vectors for delivery of transgenic proteins to differAd 5, while 95.9% took up Ad 41. In studies with differenentiated enterocytes.

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Section: Introductionmentioning

confidence: 99%

In vitro and in vivo asessment of adenovirus 41 as a vector for gene delivery to the intestine

et al. 1998

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“…They predominantly infect infants and young children, and appear to be endemic in most countries (Kidd et al, 1983;Shinozaki et al, 1987). A peculiar twist to their pattern of infection is that Ad41 identifications in European countries during the 1980s came to outnumber Ad40 identifications by several fold (de dung et al, 1993Allard et al, 1985;van Loon et al, 1985;van der Avoort et al, 1989;Scott-Taylor et al, 1992) genomic DNA have been constructed. Restriction enzyme sites for Ad40 were mapped using BamHI, EcoRI, HindlII, KpnI, Sinai and XhoI (Takiff et al, 1984;van der Avoort et al, 1989).…”

Section: Classificationmentioning

confidence: 99%

“…Maps for EcoRI, SalI, ClaI, BclI, BstEII and PvuI digestion of Ad40 DNA were constructed by van Loon et al (1985). Restriction enzyme sites for Ad41 were mapped using BamHI (Takiff et al, 1984;Allard et al, 1985;van der Avoort et al, 1989;Scott-Taylor et al, 1992), EcoRI (Takiff et al, 1984;Allard et al, 1985;van Loon et al, 1985;van der Avoort et al, 1989;Scott-Taylor et al, 1992), HpaI, NruI, PvuI (Allard et al, 1985), SalI (Allard et al, 1985;van Loon et al, 1985;Scott-Taylor et al, 1992), KpnI (Takiff et al, 1984;van der Avoort et al, 1989), XhoI (Takiff et al, 1984;van Loon et al, 1985;van der Avoort et al, 1989: Scott-Taylor et al, 1992, ClaI (van Loon et al, 1985;Scott-Taylor et al, 1992), PstI (van der Avoort et al, 1989;Scott-Taylor et al, 1992), HindIII and Sinai (Takiff et al, 1984;van der Avoort et al, 1989;Scott-Taylor et al, 1992) and BgIII (Scott-Taylor et al, 1992). The total length of the DNA was estimated to be 34.0 kb for Ad40 and 34.7 kb for Ad41 (van Loon et al, 1985).…”

Section: Classificationmentioning

confidence: 99%

The subgroup F adenoviruses

Tiemessen

Kidd

1995

Journal of General Virology

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Detection of enteric adenoviruses with synthetic oligonucleotide probes

Scott-Taylor

Ahluwalia

Dawood

et al. 1993

Journal of Medical Virology

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The abilities of hybridization probes to detect all human adenovirus types and to identify enteric adenovirus types were evaluated. The efficiency of hybridization was compared to other tests currently in routine laboratory use on clinical specimens from young children with gastroenteritis. Probes were derived from various regions of the adenovirus types 2 and 41 genomes, and were evaluated by hybridization with a series of DNA quantities from 1 microgram to 10 pg of one adenovirus type from each human subgenus, lambda phage, and HEp 2 cells. The sensitivity of hybridization with the HPII probe (92.7%), containing the conserved hexon gene, compared well with EM (54.6%), culture and neutralization (45.5%), and enzyme immunoassay (61.8%). The sensitivity of detection of enteric adenovirus isolates by the cloned Bg/II D fragment probe (92.9%) and by a synthetic probe (85.7%), manufactured from type-specific sequences of the Ad41 hexon gene were comparable to Ad40/Ad41 specific enzyme immunoassay (84.6%). Hybridization was found to be a sensitive method of adenovirus detection in comparison to traditional methods of laboratory diagnosis. Synthetic oligonucleotides enable specific detection of individual enteric adenovirus types. Hybridization had additional advantages over other tests in identifying cases of infection with more than one adenovirus type and in allowing an estimate of the concentration of adenovirus in the specimen.

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Restriction analysis of the prototype strain of enteric adenovirus type 41 using exonuclease III

Cited by 5 publications

References 22 publications

In vitro and in vivo asessment of adenovirus 41 as a vector for gene delivery to the intestine

In vitro and in vivo asessment of adenovirus 41 as a vector for gene delivery to the intestine

The subgroup F adenoviruses

Detection of enteric adenoviruses with synthetic oligonucleotide probes

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