Restricted dendritic cell and monocyte progenitors in human cord blood and bone marrow

Abstract

Liu, Nussenzweig, and colleagues track the differentiation of human progenitor cells into dendritic cells (DCs). They show that a granulocyte/monocyte/DC progenitor gives rise to a monocyte-DC progenitor that in turn gives rise to both monocytes and a common DC progenitor. The common DC progenitor produces the three major subsets of human DCs.

Macrophages and osteoclasts stem from a bipotent progenitor downstream of a macrophage/osteoclast/dendritic cell progenitor

“…We recently described the human (h)MODP 15 and noted that its cell surface phenotype was similar to that of the hCDP that was described shortly before that 28 ( Figure 7H). Both progenitor populations are very rare, representing about 0.2% of total BM ( Figure 7I).…”

“…These data, together with gene expression profiling, confirmed without bias the existence of the MODP and MOP and their developmental relationship. They also revealed that the mouse 23,24 and human 28 CDP populations are similar, respectively identical to the MODP, and that the mouse MDP population 22 is partially overlapping with the MOP and indeed devoid of DC potential.…”

“…Human CDPs 28 were sorted and cultured at 1000 cells per well in 96-well plates (BD Falcon) under OC or MF differentiation conditions, as has been previously described. 15 For OC differentiation, cells were cultured in a-MEM with 10% FCS, 25 ng/mL recombinant human (rh) M-CSF, and 40 ng/mL rh RANKL.…”

A functional DC cross talk promotes human ILC homeostasis in humanized mice

“…34,35 This population was detected in the BM of BRGSF mice (supplemental Figure 2B), and its frequency and total numbers increased following Flt3L treatment (supplemental Figure 2C). These data suggest that the increased frequency and absolute numbers of myeloid cells in Flt3L-treated BRGSF HIS mice may result from an increase in the CD34…”

Vitamin D immunoregulation through dendritic cells

“…Protocols to differentiate moDC use purification of CD14 + blood cells, and most likely omit dedicated DC precursors, which circulate in human peripheral blood in low numbers. These blood‐borne pre‐DC can be induced to differentiate into bona fide DC ex vivo 60, 61. For future DC‐based immunotherapy, it will be of interest to compare the tolerogenic potential of preDC‐derived DC and monocyte‐derived DC generated ex vivo with or without VD3.…”