Restraint stress up-regulates expression of zinc transporter Zip14 mRNA in mouse liver

Ohashi, Takuya; Matsui, Tohru; Chujo, Moeko; Nagao, Masaya

doi:10.1007/s10616-008-9148-x

Cited by 7 publications

(6 citation statements)

References 13 publications

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“…Overall, circulating zinc concentrations were reduced whilst liver zinc was significantly higher in all stress groups compared to unstressed controls. These results agree with previous studies which employed both acute and chronic stress models in addition to in vitro studies using primary hepatocytes treated with synthetic glucocorticoids [ 27 , 55 , 56 , 57 ]. Furthermore, zinc deficiency has been observed in individuals suffering from anxiety and depression, whilst a zinc-deficient diet has been shown to influence the severity of depressive symptoms in women but not men [ 58 , 59 , 60 ].…”

Section: Discussionsupporting

confidence: 92%

“…Interestingly, the zinc exporter ZnT1 showed an initial increase in mRNA levels before becoming significantly downregulated following repeated stress exposure which could facilitate hepatic zinc sequestration in these longer stress durations. Zinc accumulation by the liver is also supported by the significant increase in the zinc importer, Zip14, which is comparable with previous studies [ 27 , 56 ]. Furthermore, Liuzzi and colleagues [ 66 ] have shown that hepatic Zip14 is highly responsive to inflammatory cytokines including interleukin-6 and is responsible for producing the hypozincemia in circulation observed in an acute phase response.…”

Section: Discussionsupporting

confidence: 91%

“…Specifically, Mtf1 is a key regulator of intracellular zinc that induces changes in the transcription of transporters such as ZnT1 and metallothionein in response to elevated zinc content and other stress stimuli such as oxidative stress and hypoxia [ 24 , 25 , 26 ]. Interestingly, Ohashi and colleagues [ 27 ] have demonstrated that following acute restraint stress in mice, zinc concentration in the liver was significantly elevated together with the zinc importer, Zip14, which may play a protective role against oxidative damage. While it is known that copper and iron-catalysed hydroxyl radicals are potent mediators of cellular oxidative stress, the contribution of stress on copper and iron homeostasis is less well characterised.…”

Section: Introductionmentioning

confidence: 99%

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Hepatic Homeostasis of Metal Ions Following Acute Repeated Stress Exposure in Rats

et al. 2021

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Essential metals such as copper, iron, and zinc are cofactors in various biological processes including oxygen utilisation, cell growth, and biomolecular synthesis. The homeostasis of these essential metals is carefully controlled through a system of protein transporters involved in the uptake, storage, and secretion. Some metal ions can be transformed by processes including reduction/oxidation (redox) reactions, and correspondingly, the breakdown of metal ion homeostasis can lead to formation of reactive oxygen and nitrogen species. We have previously demonstrated rapid biochemical responses to stress involving alterations in the redox state to generate free radicals and the resultant oxidative stress. However, the effects of stress on redox-active metals including iron and copper and redox-inert zinc have not been well characterised. Therefore, this study aims to examine the changes in these essential metals following exposure to short-term repeated stress, and to further elucidate the alterations in metal homeostasis through expression analysis of different metal transporters. Outbred male Wistar rats were exposed to unrestrained (control), 1 day, or 3 days of 6 h restraint stress (n = 8 per group). After the respective stress treatment, blood and liver samples were collected for the analysis of biometal concentrations and relative gene expression of metal transporter and binding proteins. Exposure to repeated restraint stress was highly effective in causing hepatic redox imbalance. Stress was also shown to induce hepatic metal redistribution, while modulating the mRNA levels of key metal transporters. Overall, this study is the first to characterise the gene expression profile of metal homeostasis following stress and provide insight into the changes occurring prior to the onset of chronic stress conditions.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

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Hepatic Homeostasis of Metal Ions Following Acute Repeated Stress Exposure in Rats

et al. 2021

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“…Markers that show stress at an early stage will therefore be useful for diagnostic purposes. Researchers have attempted to establish methods to evaluate both pathological and physiological stress using biochemical markers, such as the concentrations of ACTH, catecholamine, galectin-1 prolactin, leptin, chromogranin A, and cortisol in the peripheral blood, saliva or urine (8)(9)(10).…”

Section: Introductionmentioning

confidence: 99%

Stress and Anxiety in Children After the Use of Computerized Dental Anesthesia

et al. 2015

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The aim of this study was to evaluate the transitory stress levels and the anxiety state in children submitted to conventional and computerized dental anesthesia. Twenty children (7 to 12 years) were randomly assigned to receive conventional and computerized dental anesthesia. To investigate the hypothesis that transitory stress could be lower after using computerized anesthesia compared to conventional anesthesia, cortisol levels in saliva were measured before and after each technique. Anxiety was also evaluated individually by answering the State-Trait Anxiety Inventory for Children (STAIC). Numerical data were analyzed statistically by the Mann-Whitney non-parametric test (5% significance level). Salivary cortisol levels increased in 8 (40%) patients after conventional anesthesia and in 9 (45%) patients after computerized anesthesia, with no statistically significant difference between the two types (p=0.34). In the same way, no statistically significant difference was found between the techniques (p=0.39) related to the psychological analysis based on the STAIC scores. Local anesthesia using either conventional anesthesia or a computerized delivery system produced similar level of stress/anxiety in pediatric patients, using both quantitative and qualitative analyses.

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“…Some experiments showed that the zinc concentration in the liver was significantly higher in mice at 12 h after the onset of restraint stress than that without stress. The interleukin-6 (IL-6) protein level in the serum was transiently elevated at 3 h after the onset of restraint stress, and the IL-6-responsive zinc transporter ZRT/IRT-like Protein 14 (ZIP14) mRNA in the liver was expressed markedly at 6 h [ 19 ]. These results suggest that ZIP14 plays a major role in the mechanism responsible for accumulation of zinc in the liver under restraint stress.…”

Section: Introductionmentioning

confidence: 99%

Psychological stress induced zinc accumulation and up-regulation of ZIP14 and metallothionein in rat liver

Xue

Zheng

et al. 2014

BMC Gastroenterol

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BackgroundZinc is necessary for normal liver function; and vice versa, the liver plays a central role in zinc homeostasis. The aim of present study is to assess the effects of repeated psychological stress (PS) on the zinc metabolism and related mechanism involved in zinc homeostasis in rat liver.MethodsIn present study, we used communication box to create PS model and investigated the serum corticosterone (CORT), zinc level in serum and liver, liver metallothionein (MT) content and ZRT/IRT-like Protein 14 (ZIP14) mRNA expression.ResultsThe results showed that the serum CORT level increased and serum zinc level decreased significantly after 7 d and 14 d PS treatment. Meanwhile, zinc and MT contents in liver were elevated after 14 d PS exposure, while those in 7 d PS exposure group did not change. ZIP14 mRNA was expressed markedly at 7 d after the onset of PS, while Zip14 mRNA expression in the liver after 14 d PS exposure reached normal level compared with control group.ConclusionsThe results suggest that PS exposure could induce hypozincemia, which might be related to liver zinc accumulation because of high level of MT through glucocorticoid-mediated MT synthesis and ZIP14 expression induced by interleukin-6.

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Restraint stress up-regulates expression of zinc transporter Zip14 mRNA in mouse liver

Cited by 7 publications

References 13 publications

Hepatic Homeostasis of Metal Ions Following Acute Repeated Stress Exposure in Rats

Hepatic Homeostasis of Metal Ions Following Acute Repeated Stress Exposure in Rats

Stress and Anxiety in Children After the Use of Computerized Dental Anesthesia

Psychological stress induced zinc accumulation and up-regulation of ZIP14 and metallothionein in rat liver

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