Restoring glomerular filtration rate by sulforaphane modulates ERK1/2/JNK/p38MAPK, IRF3/iNOS, Nrf2/HO-1 signaling pathways against folic acid-induced acute renal injury in rats

Zaghlool, Sameh S.; Abdelaal, Nashwa; El-Shoura, Ehab A.M.; Mahmoud, Nesreen Ishak; Ahmed, Yasmin Moustafa

doi:10.1016/j.intimp.2023.110777

Cited by 8 publications

(3 citation statements)

References 49 publications

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“…The NRF2/HO-1 signaling pathway participates in the regulation of various stress environments by regulating antioxidant and phase 2 detoxifying enzymes, in addition to related proteins [56,57]. Under oxidative stress, NRF2 activates downstream proteins such as NQO1, HO-1, and GCLM, which enhances the coupling reaction and the expression of related antioxidant enzymes (e.g., SOD, GSH-Px, and CAT), thus protecting against oxidative-stress-induced damage [58,59].…”

Section: Discussionmentioning

confidence: 99%

The Effects of Resveratrol and Apigenin on Jejunal Oxidative Injury in Ducks and on Immortalized Duck Intestinal Epithelial Cells Exposed to H2O2

Zhou,

Cao,

Luo

et al. 2024

Antioxidants

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Oxidative stress increases the apoptosis of intestinal epithelial cells and impairs intestinal epithelial cell renewal, which further promotes intestinal barrier dysfunction and even death. Extensive evidence supports that resveratrol and apigenin have antioxidant, anti-inflammatory, and antiproliferative properties. Here, we investigated the ability of these two compounds to alleviate diquat-induced jejunal oxidative stress and morphological injury, using the duck as a model, as well as the effects of apigenin on oxidative stress induced by H2O2 in immortalized duck intestinal epithelial cells (IDECs). Ducks were randomly assigned to the following four groups, with five replicates: a control (CON) group, a diquat-challenged (DIQ) group, a resveratrol (500 mg/kg) + diquat (RES) group, and an apigenin (500 mg/kg) + diquat (API) group. We found that serum catalase (CAT) activity and total antioxidant capacity (T-AOC) markedly reduced in the RES and API groups as compared to the DIQ group (p < 0.05); moreover, serum S superoxide dismutase (SOD) levels increased significantly in the API group as compared to the DIQ group (p < 0.05). In jejunal mucosa, the malondialdehyde (MDA) content in the RES and API groups decreased more than that in the DIQ group (p < 0.05). In addition, the jejunal expression levels of the NRF2 and GCLM genes in the RES and API groups increased notably compared with those in the DIQ group (p < 0.05); meanwhile, CAT activity in the RES and API groups was markedly elevated compared with that in the CON group (p < 0.05). In IDECs, apigenin significantly restrained the H2O2-mediated increase in MDA content and decrease in CAT levels (p < 0.05). Furthermore, apigenin increased the protein expression of p-NRF2, NRF2, p-AKT, and p-P38; downregulated that of cleaved caspase-3 and cleaved caspase-9; and reduced the ratio of Bax/Bcl-2 in H2O2-treated IDECs (p < 0.05). In conclusion, resveratrol and apigenin can be used as natural feed additives to protect against jejunal oxidative stress in ducks.

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Section: Discussionmentioning

confidence: 99%

The Effects of Resveratrol and Apigenin on Jejunal Oxidative Injury in Ducks and on Immortalized Duck Intestinal Epithelial Cells Exposed to H2O2

Zhou,

Cao,

Luo

et al. 2024

Antioxidants

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“…Inflammation associated with diabetes in rats has been extensively used in sulforaphane studies and includes efficacy in diabetic neuropathy [ 48 ], a diabetic model of renal inflammation [ 49 ], experimental diabetic peripheral neuropathy in rats [ 50 ], and streptozotocin (STZ)-induced diabetic rats [ 51 ]. Further research on renal inflammation in rats has included folic acid-induced acute renal injury [ 52 ] and cisplatin-induced nephropathy where the expression of pro-inflammatory cytokine (TNF-α) and nuclear factor κB (NF-κB) were suppressed [ 53 ]. Other inflammation models in rats for sulforaphane research were cancer-induced bone pain [ 54 ], carrageenan-induced oedema [ 55 ], the traumatic haemorrhagic shock model [ 56 ], chromium-induced lung injury [ 57 ], arsenic-induced nephrotoxicity [ 58 ], ioversol-induced nephropathy [ 59 ], the neuroinflammation and spatial learning model [ 60 ], age-related renal injury in rats [ 61 ], anti-nociceptive and anti-inflammatory effects on a sciatic endometriosis rat model [ 62 ], and chronic renal allograft dysfunction [ 63 ].…”

Section: Anti-inflammatory Effects In Vivomentioning

confidence: 99%

Anti-Inflammatory Therapeutic Mechanisms of Isothiocyanates: Insights from Sulforaphane

Habtemariam

2024

Biomedicines

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Isothiocyanates (ITCs) belong to a group of natural products that possess a highly reactive electrophilic -N=C=S functional group. They are stored in plants as precursor molecules, glucosinolates, which are processed by the tyrosinase enzyme upon plant tissue damage to release ITCs, along with other products. Isolated from broccoli, sulforaphane is by far the most studied antioxidant ITC, acting primarily through the induction of a transcription factor, the nuclear factor erythroid 2–related factor 2 (Nrf2), which upregulates downstream antioxidant genes/proteins. Paradoxically, sulforaphane, as a pro-oxidant compound, can also increase the levels of reactive oxygen species, a mechanism which is attributed to its anticancer effect. Beyond highlighting the common pro-oxidant and antioxidant effects of sulforaphane, the present paper was designed to assess the diverse anti-inflammatory mechanisms reported to date using a variety of in vitro and in vivo experimental models. Sulforaphane downregulates the expression of pro-inflammatory cytokines, chemokines, adhesion molecules, cycloxyhenase-2, and inducible nitric oxide synthase. The signalling pathways of nuclear factor κB, activator protein 1, sirtuins 1, silent information regulator sirtuin 1 and 3, and microRNAs are among those affected by sulforaphane. These anti-inflammatory actions are sometimes due to direct action via interaction with the sulfhydryl structural moiety of cysteine residues in enzymes/proteins. The following are among the topics discussed in this paper: paradoxical signalling pathways such as the immunosuppressant or immunostimulant mechanisms; crosstalk between the oxidative and inflammatory pathways; and effects dependent on health and disease states.

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“…Additionally, in patients with type 2 diabetes mellitus, it is possible that a deficiency in folic acid could potentially heighten the likelihood of elevated TSH levels, and low serum folate levels have been associated with nonalcoholic fatty liver disease and advanced hepatic fibrosis . At the same time, folic acid induces acute kidney injury (AKI), which is widely used in medicine as a reproducible model of AKI . Consequently, ensuring adequate folic acid supplementation is of utmost importance to promote proper development, growth, and overall well-being among various individuals, particularly pregnant women, infants, and the elderly.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of In Vivo Folic Acid Bioavailability in Different Mouse Strains Using Enzymatic Digestion Combined with Ultra Performance Liquid Chromatography

Qi,

Xu,

Mao

et al. 2024

J. Agric. Food Chem.

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By analyzing the folic acid content of various mouse strains through the use of in vivo studies, this study sought to determine whether folic acid bioavailability varies between hosts. In order to examine the stability of folic acid in the gastrointestinal tract, the rate at which it enters the blood, its retention in the organs, and its entry into the brain, folic acid was gavaged for 10 days into male and female mice of the following four strains: C57BL/6, BALB/c, ICR, and Kunming. Folic acid was extracted from eight groups of mice via solid phase extraction and triple enzyme extraction; the folic acid was subsequently quantified by ultraperformance liquid chromatography. In contrast to the other groups, female C57BL/6 mice exhibited substantially greater bioavailability as well as variations in organ retention and blood entry rates, as indicated by the experimental findings. This finding indicated that using female C57BL/6 mice to evaluate the bioavailability of folic acid is more effective.

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Restoring glomerular filtration rate by sulforaphane modulates ERK1/2/JNK/p38MAPK, IRF3/iNOS, Nrf2/HO-1 signaling pathways against folic acid-induced acute renal injury in rats

Cited by 8 publications

References 49 publications

The Effects of Resveratrol and Apigenin on Jejunal Oxidative Injury in Ducks and on Immortalized Duck Intestinal Epithelial Cells Exposed to H2O2

The Effects of Resveratrol and Apigenin on Jejunal Oxidative Injury in Ducks and on Immortalized Duck Intestinal Epithelial Cells Exposed to H2O2

Anti-Inflammatory Therapeutic Mechanisms of Isothiocyanates: Insights from Sulforaphane

Evaluation of In Vivo Folic Acid Bioavailability in Different Mouse Strains Using Enzymatic Digestion Combined with Ultra Performance Liquid Chromatography

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