Restorer breeding in sweet pepper: Introgressing Rf allele from hot pepper through marker-assisted backcrossing

Lin, Shih-Wen; Shieh, H.C.; Wang, Yen-Wei; Tan, Chee-Wee; Schafleitner, Roland; Yang, Wen-Ju; Kumar, Sanjeet

doi:10.1016/j.scienta.2015.09.036

Cited by 24 publications

(16 citation statements)

References 12 publications

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“…Recently, Lin et al. () introgressed the Rf allele from hot pepper into several sweet pepper lines opening the way to an efficient CMS application for sweet pepper hybrid seed production.…”

Section: Pollination Control Methods In Different Vegetable Cropsmentioning

confidence: 99%

“…CGMS is commercially used to produce hot (chilli) pepper hybrid seed, but it has not been successful for sweet pepper due to the lack of restorer genes or instability of restorer lines in most of sweet pepper genotypes. Recently, Lin et al (2015) introgressed the Rf allele from hot pepper into several sweet pepper lines opening the way to an efficient CMS application for sweet pepper hybrid seed production.…”

Section: Solanaceaementioning

confidence: 99%

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The use of genetic, manual and chemical methods to control pollination in vegetable hybrid seed production: a review

Colombo¹,

Galmarini

2017

Plant Breeding

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Production of hybrid varieties of vegetable crops is currently a desired breeding goal, due to their remarkable agronomic performance and to the possibility of intellectual property protection. However, efficient hybrid production requires a careful pollination control to guarantee the hybrid nature of F 1 seed. Several technologies ranging from manual emasculation to genetic transformation are used to inhibit pollen production in mother plants. In this review, we examine the principles underlying strategies like genetically determined systems (genic male sterility, cytoplasmic-genic male sterility, self-incompatibility) and other methods (manual emasculation, chemical-hybridizing agents) in different species, considering the benefits and drawbacks of their adoption. Finally, we present the current state of the art for vegetable hybrid seed production.

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“…Recently, Lin et al. () introgressed the Rf allele from hot pepper into several sweet pepper lines opening the way to an efficient CMS application for sweet pepper hybrid seed production.…”

Section: Pollination Control Methods In Different Vegetable Cropsmentioning

confidence: 99%

Section: Solanaceaementioning

confidence: 99%

The use of genetic, manual and chemical methods to control pollination in vegetable hybrid seed production: a review

Colombo¹,

Galmarini

2017

Plant Breeding

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“…Despite this, the study of CMS and Rf interaction in chile pepper is complex and has had inconsistent results (Min et al, 2008). One major issue with the CMS and Rf system in Capsicum species is that they seem to be less stable in sweet pepper than in chile pepper (Lin et al, 2015).…”

mentioning

confidence: 99%

“…Min et al (2009) reported the markers OPP14 and AFR4 were closely linked to Rf in a given population; however, they were unable to accurately detect Rf in diverse chile pepper lines. The CRF-SCAR (Gulyas et al, 2006) has been reported to be the most widely applicable indicator of the markers that are available (Jiang, 2015;Jo et al, 2010;Kim et al, 2009;Lin et al, 2015). The CRF-SCAR marker has been used for markerassisted selection to introduce an Rf gene into novel germplasm (Lin et al, 2015).…”

mentioning

confidence: 99%

“…The CRF-SCAR (Gulyas et al, 2006) has been reported to be the most widely applicable indicator of the markers that are available (Jiang, 2015;Jo et al, 2010;Kim et al, 2009;Lin et al, 2015). The CRF-SCAR marker has been used for markerassisted selection to introduce an Rf gene into novel germplasm (Lin et al, 2015). More recently, the Co1Mod1 marker (Jo et al, 2016) has been found to have similar applicability.…”

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confidence: 99%

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Genome-wide Identification of Chile Pepper Pentatricopeptide Repeat Domains Provides Insight into Fertility Restoration

Barchenger¹,

Said²,

Zhang³

et al. 2018

J. Amer. Soc. Hort. Sci.

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ADDITIONAL INDEX WORDS. bioinformatics, chile pepper, cytoplasmic male sterility, hybrid breeding, PPR, restorer of fertility, single nucleotide polymorphism ABSTRACT. Pentatricopeptide repeat (PPR) proteins constitute one of the largest protein families in plants. They are typically targeted to the mitochondria or chloroplast and bind to one or more organellar transcripts, influencing expression. Genes responsible for inhibiting the mitochondrial genes that cause cytoplasmic male sterility (CMS), restorer-of-fertility (Rf) genes, often encode PPRs. Using in silico techniques, 552 PPR domains were identified throughout the chile pepper (Capsicum annuum) genome. The domains were mapped across 12 chromosomes and were found to be largely distally or proximally located. About 28% of the chile pepper PPR domains identified in this study have high structural similarity to previously reported PPRs in arabidopsis (Arabidopsis thaliana). In addition, 11 candidate Rf genes clustered on chromosome 6, and 1 on chromosome 1 were identified that were characterized in 16 A-(S rfrf), B-(N rfrf), and C-line (N RfRf) backgrounds. These findings support a multigene model for fertility restoration and broaden our understanding of the restoration of fertility. This may be an explanation for the lack of widely applicable molecular markers for this important trait. With this new information, specific Rf markers may be developed and will facilitate the implementation of hybrid breeding programs in chile pepper. In addition, this work provides a basis for future research in PPRs, an increasingly important gene family.

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Conventional and Contemporary Approaches to Enhance Efficiency in Breeding Chilli/Hot Pepper

Rao

Kumar

2020

Accelerated Plant Breeding, Volume 2

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Restorer breeding in sweet pepper: Introgressing Rf allele from hot pepper through marker-assisted backcrossing

Cited by 24 publications

References 12 publications

The use of genetic, manual and chemical methods to control pollination in vegetable hybrid seed production: a review

The use of genetic, manual and chemical methods to control pollination in vegetable hybrid seed production: a review

Genome-wide Identification of Chile Pepper Pentatricopeptide Repeat Domains Provides Insight into Fertility Restoration

Conventional and Contemporary Approaches to Enhance Efficiency in Breeding Chilli/Hot Pepper

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