Restorative effects of human placenta extract in X-ray-irradiated mice.

Mochizuki, Hajime; Kada, Tsuneo

doi:10.1269/jrr.23.403

Cited by 5 publications

(3 citation statements)

References 7 publications

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“…Considering the anti-oxidative and anti-inflammatory activities of placental extracts, it is plausible that placental extracts can also effectively protect from radiation injury. In fact, it has been previously demonstrated that the oral administration of placental extracts significantly improved the survival of mice exposed to high-dose irradiation [22]. Furthermore, placental extracts have also been reported to effectively attenuate radiation-induced oral mucositis and acute radiodermatitis in patients [23, 24].…”

Section: Introductionmentioning

confidence: 99%

Placental extract protects bone marrow-derived stem/progenitor cells against radiation injury through anti-inflammatory activity

Kawakatsu

Urata

Goto

et al. 2012

Journal of Radiation Research

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Placental extracts have been reported to have anti-oxidative and anti-inflammatory activities. Because there is increasing evidence that ionizing radiation induces the release of reactive oxygen species (ROS) and inflammatory cytokines, we examined the protective effects of a placental extract against radiation injury. C57BL/6 mice were exposed to 1 Gy of γ-ray radiation every day for 5 days, and placental extract (1 mg/day) was administrated orally soon after each exposure. At 2 days after the last irradiation, mice were euthanized to examine the numbers, colony-forming capacity, and DNA damage of stem/progenitor cells in the peripheral blood and bone marrow. To understand the related mechanisms, we also measured the levels of intracellular and mitochondrial ROS, and 8-OHdG in the plasma and urine, and IL-6 and TNF-α in the plasma. Compared with the placebo treatment, oral administration of placental extract significantly increased the number and colony-forming capacity, but decreased the DNA damage of bone marrow stem/progenitor cells. However, neither the levels of intracellular and mitochondrial ROS in bone marrow cells, nor the levels of 8-OHdG in the urine and plasma significantly differed between groups. Interestingly, in comparison with the placebo treatment, placental extract significantly decreased the levels of the inflammatory cytokines IL-6 and TNF-α in the plasma. Placental extract significantly attenuated the acute radiation injury to bone marrow-derived stem/progenitor cells, and this protection is likely to be related to the anti-inflammatory activity of the placental extract.

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Section: Introductionmentioning

confidence: 99%

Placental extract protects bone marrow-derived stem/progenitor cells against radiation injury through anti-inflammatory activity

Kawakatsu

Urata

Goto

et al. 2012

Journal of Radiation Research

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“…Therefore, the human placenta contains antioxidant defense substances to protect embryos from oxidative stress ( 62 – 64 ). Human placenta extract prepared from the placenta of healthy pregnant females is known to have various physiological actions, including antioxidative properties ( 57 , 65 ). In the present study, hPH was prepared with human placenta, including umbilical cord, following the provision of consent from the pregnant women.…”

Section: Discussionmentioning

confidence: 99%

Anti‑apoptotic effects of human placental hydrolysate against hepatocyte toxicity in�vivo and in�vitro

Bak

Choi

et al. 2018

Int J Mol Med

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Apoptosis and oxidative stress are essential for the pathogenesis of acute liver failure and fulminant hepatic failure. Human placental hydrolysate (hPH) has been reported to possess antioxidant and anti-inflammatory properties. In the present study, the protective effects of hPH against D-galactosamine (D-GalN)- and lipopolysaccharide (LPS)-induced hepatocyte apoptosis were investigated in vivo. In addition, the molecular mechanisms underlying the anti-apoptotic activities of hPH against D-GalN-induced cell death in vitro were examined. Male Sprague-Dawley rats were injected with D-GaIN/LPS with or without the administration of hPH. Rats were sacrificed 24 h after D-GaIN/LPS intraperitoneal injection, and the blood and liver samples were collected for future inflammation and hepatotoxicity analyses. Changes in cell viability, apoptosis protein expression, mitochondrial mass, mitochondrial membrane potential, reactive oxygen species generation, and the levels of proteins and mRNA associated with a protective mechanism were determined in HepG2 cells pretreated with hPH for 2 h prior to D-GalN exposure. The findings suggested that hPH treatment effectively protected against D-GalN/LPS-induced hepatocyte apoptosis by reducing the levels of alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase, interleukin-6, and tumor necrosis factor-α, and increasing the level of proliferating cell nuclear antigen. It was also found that hPH inhibited the apoptotic cell death induced by D-GalN. hPH activated the expression of antioxidant enzymes, including superoxide dismutase, glutathione peroxidase, and catalase, which were further upregulated by the Kelch-like ECH2-associated protein 1-p62-nuclear factor-erythroid 2-related factor 2 pathway, a component of oxidative stress defense mechanisms. Furthermore, hPH markedly reduced cytosolic and mitochondrial reactive oxygen species and rescued mitochondrial loss and dysfunction through the reduction of damage-regulated autophagy modulator, p53, and C/EBP homologous protein. Collectively, hPH exhibited a protective role in hepatocyte apoptosis by inhibiting oxidative stress and maintaining cell homeostasis. The underlying mechanisms may be associated with the inhibition of endoplasmic reticulum stress and minimization of the autophagy progress.

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“…Several clinical investigations and findings have been reported on effective therapeutic use of placental extract such as clinical evaluation in radiation-induced oral mucositis (Kaushal et al, 2001), restorative effects in X-ray-irradiated mice (Mochizuki and Kada, 1982), for the treatment of myopic and senile chorio-retinal dystrophies (Girotto and Malinverni, 1982), rheumatic arthritis (Rosenthal, 1982), osteoarthritis , skin diseases (Punshi, 1981), for prevention of recurring respiratory infections (Lo Polito, 1980), in the therapy of urticarias (Mittal, 1977), in therapy of children with asthmatic bronchitis (Vecchi et al, 1977), in atrophic rhinitis (Sinha et al, 1976), on hepatic drug metabolizing enzymes (Bishayee et al, 1995), in cerebral arteriosclerosis (Lafay 1965), in periodontal disease (Calvarano et al, 1989). Topical treatment of cervico-vaginal lesions using a placental extract with anticomplement activity (Sgro, 1978), local therapy of psoriasis (Lodi et al, 1986), treatment of vitiligo with topical melagenine (Suite and Quamina, 1991) has also been reported to be successful.…”

Section: Multiple Therapeutic Properties Of Aqueous Extract Of Human mentioning

confidence: 99%

Aqueous Extract of Human Placenta

Chakraborty¹,

Bhattacharyya²

2012

Recent Advances in Research on the Human Placenta

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Restorative effects of human placenta extract in X-ray-irradiated mice.

Cited by 5 publications

References 7 publications

Placental extract protects bone marrow-derived stem/progenitor cells against radiation injury through anti-inflammatory activity

Placental extract protects bone marrow-derived stem/progenitor cells against radiation injury through anti-inflammatory activity

Anti‑apoptotic effects of human placental hydrolysate against hepatocyte toxicity in�vivo and in�vitro

Aqueous Extract of Human Placenta

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