Restoration of Several Morphological Characteristics of Normal Fibroblasts in Sarcoma Cells Treated with Adenosine-3′:5′-Cyclic Monophosphate and Its Derivatives

Johnson, George S.; Friedman, Robert M.; Pastan, Ira

doi:10.1073/pnas.68.2.425

Cited by 400 publications

(73 citation statements)

References 12 publications

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“…11) (Fig. 12), a result identical with that obtained by Dontenwill, Chevalier and Reckzeh (1973) (Johnson, Friedman and Pastan, 1971;Hsie and Puck, 1971;Macintyre et al, 1972). For example, these transformed cells which have the ability to form multilayers with random cell orientation and strong agglutinability by wheat germ lipase recover many of the properties of normal fibroblasts following treatment with dibutyryl cyclic AMP, forming monolayers with parallel orientation and acquiring weak agglutinability by wheat germ lipase.…”

Section: Other Tissuessupporting

confidence: 80%

Measurement of the Ability of Cells to Infiltrate Normal Tissues in vitro

Easty¹,

Easty²

1974

Br J Cancer

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Organ cultures of chorioallantoic membranes of hen eggs have been used to establish a quantitative method of measuring the infiltrative ability of a variety of normal and tumour cells. Normal fibroblasts, mouse peritoneal cells and cells of low tumorigenicity infiltrated poorly and slowly whereas most tumours infiltrated rapidly. Some cells of the more invasive tumours achieved minimum rates of migration through the normal tissue of 2-3 μm/h. One tumour line which tended to form aggregates on the chorioallantoic membrane elicited a pronounced rejection response from the ectoderm of the membrane. Colcemid, which inhibits the formation of cell processes and the directional migration of cells, and dibutyryl cyclic AMP, which restores certain aspects of normal behaviour to tumour cells in vitro, had little effect on the invasion of the membrane by tumour cells. Images Fig. 9 Fig. 10 Fig. 11 Fig. 12 Fig. 5 Fig. 6 Fig. 7 Fig. 8

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Section: Other Tissuessupporting

confidence: 80%

Measurement of the Ability of Cells to Infiltrate Normal Tissues in vitro

Easty¹,

Easty²

1974

Br J Cancer

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“…8) . DISCUSSION The results in this paper lend considerable support to the previously suggested idea that cAMP is involved in density-dependent inhibition of cell proliferation (13,20,29,30) . Early events in Gi leading to DNA synthesis in cultures released from contact-inhibition by refeeding were inhibited by theophylline, db-cAMP, and cAMP (Fig .…”

Section: Kinetics Of Irreversible Commitment To Dna Synthesissupporting

confidence: 76%

“…In addition, the proliferation of L cells and HeLa cells (26) is inhibited by exogenous cAMP . Recently N 1 , 02-dibutyryl-cAMP (db-cAMP) has been shown to restore normal growth characteristics to cells from a Rous sarcoma virus-induced rat sarcoma (13) and to 3T3 cells which were transformed either spontaneously or by polyoma virus (29) . Finally, cAMP levels in normal 3T3 cells have been found to be higher than those found in transformed 3T3 cells (20,30) .…”

Section: Introductionmentioning

confidence: 99%

Effect of Adenosine 3'-5'-Cyclic Monophosphate on Cell Proliferation

Froehlich

Rachmeler

1972

The Journal of Cell Biology

140

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Secondary cultures of human diploid fibroblasts, which demonstrate density-dependent inhibition of cell growth, were used to study the effect of adenosine 3'-5'-cyclic monophosphate (cAMP) on cell proliferation. DNA synthesis in nonconfluent cultures and in contact-inhibited cultures stimulated to grow by refeeding with fresh medium was found to be inhibited by exogenous cAMP. The properties of this inhibition of DNA synthesis, together with the alterations in cAMP metabolism observed in confluent cultures of cells stimulated with fresh medium to resume growth, strongly suggest that cAMP is involved in contact-inhibition of cell proliferation.

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“…In vitro, exogenous cGMP stimulates the proliferation of fibroblasts and lymphoid cells Whitfield et al, 1971;Diamantstein & Ulmer, 1975;Watson, 1975) whereas cAMP, or agents which increase its intracellular concentration, inhibit cell growth (Ryan & Heidrick, 1968;Yang & Vas, 1971;Pardee, 1974) and induce in malignant cells a normalappearing morphological or biochemical differentiation (Hsie & Puck, 1971;Johnson et al, 1971;Prasad et al, 1975).…”

mentioning

confidence: 99%

Patterns of cyclic nucleotides in normal and leukaemic human leucocytes

Peracchi¹,

Maiolo²,

Lombardi³

et al. 1980

Br J Cancer

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Summary.-Because recent observations indicate that metabolism of cyclic nucleotides may be altered in neoplastic cells, the intracellular levels of cyclic adenosine 3',5'-monophosphate (cAMP) and cyclic guanosine 3',5'-monophosphate (cGMP) were measured in mononuclear leukaemic and normal human leucocytes. The activities of adenylate cyclase, guanylate cyclase and cyclic nucleotide phosphodiesterases were also determined. Under basal conditions, cAMP levels were always higher in the normal leucocytes, whilst cGMP levels were of the same order of magnitude in both normal and leukaemic cells, causing the cAMP/cGMP ratios to be significantly lower in leukaemic leucocytes. Leukaemic cells significantly increased cyclic nucleotide levels in response to theophylline, but did not respond to serotonin, carbamylcholine or D,L-isoproterenol. Preincubation of these leucocytes with theophylline produced a detectable cAMP response to D,L-isoproterenol but no cGMP response to serotonin or carbamylcholine was found. Adenylate cyclase and guanylate cyclase were significantly lower in leukaemic than in normal cells, which could largely explain the abnormal cyclic nucleotide pattern found in human leukaemic leucocytes. In our experiments, cAMP phosphodiesterase activity was comparable in normal and leukaemic cells, whereas cGMP phosphodiesterase activity was undetectable in all mononuclear-leucocyte preparations with the methods used.

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Restoration of Several Morphological Characteristics of Normal Fibroblasts in Sarcoma Cells Treated with Adenosine-3′:5′-Cyclic Monophosphate and Its Derivatives

Abstract: ABSTRACT

Cited by 400 publications

References 12 publications

Measurement of the Ability of Cells to Infiltrate Normal Tissues in vitro

Measurement of the Ability of Cells to Infiltrate Normal Tissues in vitro

Effect of Adenosine 3'-5'-Cyclic Monophosphate on Cell Proliferation

Patterns of cyclic nucleotides in normal and leukaemic human leucocytes

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