Restoration of photosynthetic activity and supercomplexes from severe iron starvation in Chlamydomonas reinhardtii

Devadasu, Elsinraju; Pandey, Jayendra; Dhokne, Kunal; Subramanyam, Rajagopal

doi:10.1016/j.bbabio.2020.148331

Cited by 17 publications

(16 citation statements)

References 58 publications

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“…The entire pathway of nitrogen reduction (from either dinitrogen or nitrate) requires a flux of reducing agents from photosynthesis, resulting in an increased cellular demand for these Fe compounds [ 69 ]. It has been reported that nitrogen-fixing cyanobacteria require up to 10-fold more Fe than the same species growing at the same rate on nitrates [ 70 , 71 ].…”

Section: Discussionmentioning

confidence: 99%

Influence of Mo and Fe on Photosynthetic and Nitrogenase Activities of Nitrogen-Fixing Cyanobacteria under Nitrogen Starvation

Sadvakasova

Kossalbayev

Token

et al. 2022

Cells

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The potential of cyanobacteria to perform a variety of distinct roles vital for the biosphere, including nutrient cycling and environmental detoxification, drives interest in studying their biodiversity. Increasing soil erosion and the overuse of chemical fertilizers are global problems in developed countries. The option might be to switch to organic farming, which entails largely the use of biofertilisers. Cyanobacteria are prokaryotic, photosynthetic organisms with considerable potential, within agrobiotechnology, to produce biofertilisers. They contribute significantly to plant drought resistance and nitrogen enrichment in the soil. This study sought, isolated, and investigated nitrogen-fixing cyanobacterial strains in rice fields, and evaluated the effect of Mo and Fe on photosynthetic and nitrogenase activities under nitrogen starvation. Cyanobacterial isolates, isolated from rice paddies in Kazakhstan, were identified as Trichormus variabilis K-31 (MZ079356), Cylindrospermum badium J-8 (MZ079357), Nostoc sp. J-14 (MZ079360), Oscillatoria brevis SH-12 (MZ090011), and Tolypothrix tenuis J-1 (MZ079361). The study of the influence of various concentrations of Mo and Fe on photosynthetic and nitrogenase activities under conditions of nitrogen starvation revealed the optimal concentrations of metals that have a stimulating effect on the studied parameters.

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Section: Discussionmentioning

confidence: 99%

Influence of Mo and Fe on Photosynthetic and Nitrogenase Activities of Nitrogen-Fixing Cyanobacteria under Nitrogen Starvation

Sadvakasova

Kossalbayev

Token

et al. 2022

Cells

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“…In severe iron-deficient culture, the cells were wholly starved due to lack of iron in the medium. During the severe iron starvation, the cells were in the dividing stage, and the photosynthesis process was reduced significantly as they do not have sufficient iron content in the cell (Devadasu et al, 2021).…”

Section: Influence Of Iron Deficiency On the Growth Of Chlamydomonas Reinhardtiimentioning

confidence: 99%

Enhanced Lipid Production in Chlamydomonas reinhardtii Caused by Severe Iron Deficiency

Devadasu

Subramanyam

2021

Front. Plant Sci.

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Microalgae are used as a source of lipids for the production of biofuels. Most algae produce neutral lipids under stress conditions. Here, lipid accumulation by the unicellular alga Chlamydomonas reinhardtii was examined during cultivation under iron-limiting conditions. Severe iron stress caused the cells to accumulate a significant amount of lipid, specifically triacylglycerols (TAGs), by compromising the growth. Semi-quantitative measurements by Fourier transform infrared (FTIR) spectroscopy showed an increase in both carbohydrate and lipid content in iron-stressed C. reinhardtii cells compared to control. Analysis by flow cytometry and thin layer chromatography confirmed that severe iron deficiency-induced TAG accumulation to fourfold higher than in iron-replete control cells. This accumulation of TAGs was mostly degraded from chloroplast lipids accompanied by overexpression of diacylglycerol acyltransferase (DGAT2A) protein. Furthermore, liquid chromatography-mass spectrometry (LC-MS) analysis demonstrated significantly enhanced levels of C16:0, C18:2, and C18:3 fatty acids (FAs). These results indicate that iron stress triggers the rapid accumulation of TAGs in C. reinhardtii cells. The enhanced production of these lipids caused by the iron deficiency may contribute to the efficient production of algal biofuels if we escalate to the photobioreactor’s growth conditions.

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“…Indeed, research on the organization and assembly of the photosynthetic proteins in thylakoids of green microalgae still needs progress. Studies on this field have mainly concerned the model green alga Chlamydomonas reinhardtii (Nama et al 2015(Nama et al , 2019Devadsu et al 2021), while only a few studies have examined other species of green algae, like S. obliquus, Botryococcus braunii or N. oleoabundans under mixotrophic conditions (Kantzilakis et al 2007;Giovanardi et al 2017;van den Berg et al 2020). On the other hand, exhaustive knowledge of the photosynthetic machinery represents a valid tool for a better exploitation of photosynthetic organisms.…”

Section: Introductionmentioning

confidence: 99%

Chlorophyta microalgae as dietary protein supplement: a comparative analysis of productivity related to photosynthesis

et al. 2022

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Microalgae are studied as innovative sources of a wide range of highly valuable products, including proteins for the food/feed sectors. However, protein content varies depending on algal species, culture conditions and harvesting period. The Phylum Chlorophyta includes most of the described species of green algae. Due to their remarkable peculiarities, four Chlorophyta species belonging to two distinct classes were selected for the research: Chlorella vulgaris and Chlorella protothecoides as Trebouxiophyceae, and Neochloris oleoabundans and Scenedesmus acutus as Chlorophyceae. The algae were studied to obtain comparative results about their growth performance, and total protein content and profile under the same culture conditions. Since photosynthetic process directly influences biomass production, photosynthetic pigment, PSII maximum quantum yield and thylakoid protein content and profile were analysed. SDS-PAGE and 2D BN/SDS-PAGE were performed to expand information on the organization and assembly of the resolved thylakoid complexes of samples. Despite the algal species showed similar growth rates and photosynthetic efficiency, S. acutus showed the highest capability to accumulate proteins and photosynthetic pigments. Regarding the thylakoid protein profile, the two Trebouxiophyceae showed very similar pattern, whereas different amounts of LHCII occurred within the two Chlorophyceae. Finally, the separation of thylakoid protein complexes in 2D BN/SDS-PAGE revealed a more complex pattern in S. acutus as compared to the other species. Overall, it is suggested that a higher stability of the photosynthetic membranes can result in higher biomass and protein production. Altogether, results have highlighted the metabolic uniqueness of each strain, resulting in a non-obvious comparison with the other species.

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Restoration of photosynthetic activity and supercomplexes from severe iron starvation in Chlamydomonas reinhardtii

Cited by 17 publications

References 58 publications

Influence of Mo and Fe on Photosynthetic and Nitrogenase Activities of Nitrogen-Fixing Cyanobacteria under Nitrogen Starvation

Influence of Mo and Fe on Photosynthetic and Nitrogenase Activities of Nitrogen-Fixing Cyanobacteria under Nitrogen Starvation

Enhanced Lipid Production in Chlamydomonas reinhardtii Caused by Severe Iron Deficiency

Chlorophyta microalgae as dietary protein supplement: a comparative analysis of productivity related to photosynthesis

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