Restoration of electrical microenvironment enhances bone regeneration under diabetic conditions by modulating macrophage polarization

Dai, Xianzhong; Heng, Boon Chin; Bai, Yunyang; You, Fangtian; Sun, Xiaowen; Li, Yiping; Tang, Zhangui; Xu, Mingming; Zhang, Xuehui; Deng, Xuliang

doi:10.1016/j.bioactmat.2020.12.020

Cited by 92 publications

(91 citation statements)

References 35 publications

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“…In the present study, the electrical dipoles of piezoelectric P (VDF-TrFE) were generated by electrical poling treatment, thus creating net surface charges on the surfaces. The results were indirectly reflected by the increased β-phase contents and d 33 values, in line with previous studies (Zhang et al, 2018;Khare et al, 2020;Dai et al, 2021). Moreover, the wettability is a critical factor in determining cell behavior, which can be influenced by the surface topography and roughness (Kim et al, 2016;Kumar and Hiremath, 2019).…”

Section: Macrophage Adhesion and Morphology Affected By The Surface-charge Polarity Of P (Vdf-trfe) Membranessupporting

confidence: 90%

“…The poled membranes possessed a macroscopic polarity, resulting in a "poled −" face and a "poled +" face. The "poled -" and "poled +" faces were toward the negative and positive pole of the DC electric field and adsorbed the negative and positive polarized charges in terms of theory, respectively (Zhou et al, 2016;Khare et al, 2020;Dai et al, 2021).…”

Section: P (Vdf-trfe) Membrane Characterizationmentioning

confidence: 99%

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Differently Charged P (VDF-TrFE) Membranes Influence Osteogenesis Through Differential Immunomodulatory Function of Macrophages

et al. 2022

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A biomaterial-mediated immune response is a critical factor to determine the cell fate as well as the tissue-regenerative outcome. Although piezoelectric-membranes have attracted considerable interest in the field of guided bone regeneration thanks to their biomimetic electroactivity, the influence of their different surface-charge polarities on the immune-osteogenic microenvironment remains obscure. The present study aimed at investigating the interaction between piezoelectric poly (vinylidene fluoridetrifluoroethylene) [P (VDF-TrFE)] membranes with different surface polarities (negative or positive) and macrophage response, as well as their subsequent influence on osteogenesis from an immunomodulating perspective. Specifically, the morphology, wettability, crystal phase, piezoelectric performance, and surface potential of the synthetic P (VDF-TrFE) samples were systematically characterized. In addition, RAW 264.7 macrophages were seeded onto differently charged P (VDF-TrFE) surfaces, and the culture supernatants were used to supplement cultures of rat bone marrow mesenchymal stem cells (rBMSCs) on the corresponding P (VDF-TrFE) surfaces. Our results revealed that oppositely charged surfaces had different abilities in modulating the macrophage-immune-osteogenic microenvironment. Negatively charged P (VDF-TrFE), characterized by the highest macrophage elongation effect, induced a switch in the phenotype of macrophages from M0 (inactivated) to M2 (anti-inflammatory), thus promoting the osteogenic differentiation of rBMSCs by releasing anti-inflammatory cytokine IL-10. Interestingly, positively charged P (VDF-TrFE) possessed pro-inflammatory properties to induce an M1 (pro-inflammatory) macrophage-dominated reaction, without compromising the subsequent osteogenesis as expected. In conclusion, these findings highlighted the distinct modulatory effect of piezoelectric-P (VDF-TrFE) membranes on the macrophage phenotype, inflammatory reaction, and consequent immune-osteogenic microenvironment depending on their surface-charge polarity. This study provides significant insight into the design of effective immunoregulatory materials for the guided bone regeneration application.

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Section: Macrophage Adhesion and Morphology Affected By The Surface-charge Polarity Of P (Vdf-trfe) Membranessupporting

confidence: 90%

Section: P (Vdf-trfe) Membrane Characterizationmentioning

confidence: 99%

Differently Charged P (VDF-TrFE) Membranes Influence Osteogenesis Through Differential Immunomodulatory Function of Macrophages

et al. 2022

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“…To investigate the effects of macrophages (cultured on various scaffolds) on osteogenesis, macrophages were cultured with different scaffolds for 1 d. Then, the macrophage conditioned medium was collected. Afterwards, BMSCs were cultured in medium that made up of collected macrophage conditioned medium on different scaffolds, and Rat Mesenchymal Stem Cell Osteogenic Differentiation Medium (RAXMX-90021, Cyagen Biosciences Inc., China) at a ratio of 1:2 [ 32 ]. The procedures of ALP staining, ALP activity and ARS staining as former.…”

Section: Methodsmentioning

confidence: 99%

Polydopamine-mediated graphene oxide and nanohydroxyapatite-incorporated conductive scaffold with an immunomodulatory ability accelerates periodontal bone regeneration in diabetes

Yang

Hou

et al. 2022

Bioactive Materials

114

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“…In diabetes, for example, the inflammatory phase of the wound cannot be smoothly transitioned to the repair and remodeling phase due to the dysfunction of macrophages, leading to the persistence of the inflammatory environment and preventing the infiltration and function of repair cells. [ 26–28 ] Thus, diabetic patients may have unhealing wounds whose local microenvironment differs from the normal microenvironment in biochemical components and mechanical properties. [ 29 ] To heal injured tissues by maintaining the body's innate healing capacity, an emerging and popular approach is the use of immunomodulatory biomaterials to enhance immune‐mediated tissue regeneration, and a growing number of scholars have investigated or are investigating new ideas and strategies for clinical wound treatment and promotion of wound healing.…”

Section: Introductionmentioning

confidence: 99%

Advanced Biomaterials for Regulating Polarization of Macrophages in Wound Healing

Mao

Chen

Cai

et al. 2021

Adv Funct Materials

107

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Macrophage immunotherapy is an emerging treatment strategy that modulates the immune system to promote wound healing. The functionality and regeneration of tissue depend on spatially and temporally regulating the biophysical and biochemical microenvironmental with poorly understood mechanisms. Biomaterials are carefully crafted to display and deliver macrophage regulatory signals in a precise and near‐physiological way, serving as powerful artificial microenvironments in which to explore and direct the fate of macrophages. The review starts with discussing the classification and function of macrophages, and then introduces the polarization of macrophages in different microenvironments of conventional wounds and chronic wounds. Recent advances in biomaterials that balance the phenotypes of macrophages in wound healing are emphasized. Finally, looking ahead, the potential ability of biomaterial scaffolds to modulate immune signaling to produce an environment conducive to regeneration is discussed.

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Restoration of electrical microenvironment enhances bone regeneration under diabetic conditions by modulating macrophage polarization

Cited by 92 publications

References 35 publications

Differently Charged P (VDF-TrFE) Membranes Influence Osteogenesis Through Differential Immunomodulatory Function of Macrophages

Differently Charged P (VDF-TrFE) Membranes Influence Osteogenesis Through Differential Immunomodulatory Function of Macrophages

Polydopamine-mediated graphene oxide and nanohydroxyapatite-incorporated conductive scaffold with an immunomodulatory ability accelerates periodontal bone regeneration in diabetes

Advanced Biomaterials for Regulating Polarization of Macrophages in Wound Healing

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