Restitution of barrier and transport function of porcine colon after acute mucosal injury

Abstract

Acute injury of the porcine colonic epithelium was induced in vivo with the bile salt, deoxycholate. A concentration of 15 mM for 30 min completely destroyed the surface epithelium and induced a marked increase in mucosal permeability to mannitol. The crypt epithelium however was not significantly affected. Within 8 min of recovery, the colonic surface was reepithelialized with flattened, migrating cells, and within 40 min, mucosal permeability to mannitol was normalized. In vitro studies showed that in these early stages of recovery, NaCl transport, short-circuit current, and resistance were markedly impaired, whereas the theophylline-induced secretory response remained intact. Recovery of absorptive function paralleled the transition from flattened to columnar surface epithelium and was complete within 2 h. Results suggest that 1) active migratory events play an important role in rapid restitution of an epithelial barrier, 2) active absorption of ions is much slower to recover, and 3) active secretory events are intact and probably originate in the crypt epithelium.

Structural changes in the epithelium of the small intestine and immune cell infiltration of enteric ganglia following acute mucosal damage and local inflammation

“…Inclusion cysts occurred in the early period after the restitution of the epithelium, as is also apparent in the paper of Gaudio [23]. A rapid return of epithelium is also observed after other destructive treatments [24,25]. Experiments similar to ours, but on mouse colon, have shown re-epithelialisation at 1 day after dinitrobenzene sulphonate (DNBS; 300 mg/kg in 0.1 mL) and a similar early loss of mucin [26].…”

A report of left dorsal displacement of the large colon in a tropical horse

“…Probability of survival can be influenced by loss of the epithelial barrier, which allows transmucosal leakage of endotoxin, bacterial chemotactic peptides, and bacteria [13] . Rapid repair of the epithelium is important for recovery and involves 2 processes that are usually completed within hours: mucosal restitution and tightening of paracellular pathways between remaining cells [20,21] . Restitution involves sealing the mucosal defect with remaining viable cells before final repair through cell division and proliferation [22] .…”

Beneficial Effects of <i>L</i>-Arginine on Intestinal Epithelial Restitution after Ischemic Damage in Rats

“…the stimulation of cell migration and rearrangement in the cell layer which covers the injured mucosa [28]. Restitution was observed in the stomach where mucosal lesions are repaired within a few minutes to a few hours [28,29], both in the small intestine [17] and in the colon [30], The restitution pro cess contributes to both the morphological and functional recovery of mucosal defects. In the damaged mucosa the migrating cells come presumably from undamaged areas at the base of the villi and from the cellular sheet covering the injured mucosal surface.…”