Resting state networks of the canine brain under sevoflurane anaesthesia

Beckmann, Katrin; Wang‐Leandro, Adriano; Dennler, Matthias; Carrera, Inés; Richter, Henning; Bektas, Rima N; Steiner, Aline R; Haller, Sven

doi:10.1371/journal.pone.0231955

Cited by 12 publications

(18 citation statements)

References 60 publications

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“…This dissociation is also supported by reduced structural connectivity between anterior and posterior cingulate cortex in dogs compared to humans 63 . In the present study, these previously described dissociation could be reproduced 39 , 40 , 63 . Nevertheless the DMN in dogs is not as well studied as in humans and proof of the function of what has been identified as DMN in dogs must still be provided 38 , 39 .…”

Section: Discussionsupporting

confidence: 70%

“…Additionally, 10 research beagles were included in the healthy control group. These beagle dogs have been part of a preliminary study investigating the feasibility of RSN detection under general anaesthesia 39 .…”

Section: Methodsmentioning

confidence: 99%

“…All data were acquired as previously described 39 with a 3 Tesla scanner (Philips Ingenia, Philips AG, Zurich, Switzerland) using a 16‐channel receive‐transmit head coil (dStream HeadSpine coil solution, Philips AG). From the anatomical evaluation, a 3D T1-weighted (T1W; TR 13 ms; TE 6 ms; FOV 130 mm; slice thickness 0.6 mm; Flip angle 8°) sequence was depicted for the registration with anatomical images.…”

Section: Methodsmentioning

confidence: 99%

“…Pre-processed data were whitened and projected into a 20-dimensional subspace using Principal Component Analysis. Twenty components were chosen based on the results of our previous study 39 . The whitened observations were decomposed into sets of vectors which describe signal variation across the temporal domain (time-courses), the session/subject domain and across the spatial domain (maps) by optimising for non-Gaussian spatial source distributions using a fixed-point iteration technique 50 .…”

Section: Methodsmentioning

confidence: 99%

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Increased resting state connectivity in the anterior default mode network of idiopathic epileptic dogs

Beckmann

Wang‐Leandro

Richter

et al. 2021

Sci Rep

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Epilepsy is one of the most common chronic, neurological diseases in humans and dogs and considered to be a network disease. In human epilepsy altered functional connectivity in different large-scale networks have been identified with functional resting state magnetic resonance imaging. Since large-scale resting state networks have been consistently identified in anesthetised dogs’ application of this technique became promising in canine epilepsy research. The aim of the present study was to investigate differences in large-scale resting state networks in epileptic dogs compared to healthy controls. Our hypothesis was, that large-scale networks differ between epileptic dogs and healthy control dogs. A group of 17 dogs (Border Collies and Greater Swiss Mountain Dogs) with idiopathic epilepsy was compared to 20 healthy control dogs under a standardized sevoflurane anaesthesia protocol. Group level independent component analysis with dimensionality of 20 components, dual regression and two-sample t test were performed and revealed significantly increased functional connectivity in the anterior default mode network of idiopathic epileptic dogs compared to healthy control dogs (p = 0.00060). This group level differences between epileptic dogs and healthy control dogs identified using a rather simple data driven approach could serve as a starting point for more advanced resting state network analysis in epileptic dogs.

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Section: Discussionsupporting

confidence: 70%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Increased resting state connectivity in the anterior default mode network of idiopathic epileptic dogs

Beckmann

Wang‐Leandro

Richter

et al. 2021

Sci Rep

Self Cite

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“…The desired depth of anaesthesia may vary depending on the protocol used, but at least righting reflex should be lost under exposure to scanner noise. In dogs, 0.8 to 1.2 MAC of sevoflurane are required to immobilise and mechanically ventilate the animals (Beckmann et al, 2020). Reflexes should be checked repetitively and at the end of scan to detect lightening of anaesthesia which may have occurred during image acquisition, especially if injection anaesthesia is used.…”

Section: Additional Considerationsmentioning

confidence: 99%

Systematic Review: Anaesthetic Protocols and Management as Confounders in Rodent Blood Oxygen Level Dependent Functional Magnetic Resonance Imaging (BOLD fMRI)–Part A: Effects of Changes in Physiological Parameters

et al. 2020

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Background: To understand brain function in health and disease, functional magnetic resonance imaging (fMRI) is widely used in rodent models. Because animals need to be immobilised for image acquisition, fMRI is commonly performed under anaesthesia. The choice of anaesthetic protocols and may affect fMRI readouts, either directly or via changing physiological balance, and thereby threaten the scientific validity of fMRI in rodents. Methods: The present study systematically reviewed the literature investigating the influence of different anaesthesia regimes and changes in physiological parameters as confounders of blood oxygen level dependent (BOLD) fMRI in rats and mice. Four databases were searched, studies selected according to pre-defined criteria, and risk of bias assessed for each study. Results are reported in two separate articles; this part of the review focuses on effects of changes in physiological parameters. Results: A total of 121 publications was included, of which 49 addressed effects of changes in physiological parameters. Risk of bias was high in all included studies. Blood oxygenation [arterial partial pressure of oxygen (p a O 2)], ventilation [arterial partial pressure of carbon dioxide (p a CO 2)] and arterial blood pressure affected BOLD fMRI readouts across various experimental paradigms. Conclusions: Blood oxygenation, ventilation and arterial blood pressure should be monitored and maintained at stable physiological levels throughout experiments. Appropriate anaesthetic management and monitoring are crucial to obtain scientifically valid, reproducible results from fMRI studies in rodent models.

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Central nodes of canine functional brain networks are concentrated in the cingulate gyrus

Szabó

Janosov²,

Czeibert

et al. 2023

Brain Struct Funct

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Compared to the field of human fMRI, knowledge about functional networks in dogs is scarce. In this paper, we present the first anatomically-defined ROI (region of interest) based functional network map of the companion dog brain. We scanned 33 awake dogs in a “task-free condition”. Our trained subjects, similarly to humans, remain willingly motionless during scanning. Our goal is to provide a reference map with a current best estimate for the organisation of the cerebral cortex as measured by functional connectivity. The findings extend a previous spatial ICA (independent component analysis) study (Szabo et al. in Sci Rep 9(1):1.25. https://doi.org/10.1038/s41598-019-51752-2, 2019), with the current study including (1) more subjects and (2) improved scanning protocol to avoid asymmetric lateral distortions. In dogs, similarly to humans (Sacca et al. in J Neurosci Methods. https://doi.org/10.1016/j.jneumeth.2021.109084, 2021), ageing resulted in increasing framewise displacement (i.e. head motion) in the scanner. Despite the inherently different approaches between model-free ICA and model-based ROI, the resulting functional networks show a remarkable similarity. However, in the present study, we did not detect a designated auditory network. Instead, we identified two highly connected, lateralised multi-region networks extending to non-homotropic regions (Sylvian L, Sylvian R), including the respective auditory regions, together with the associative and sensorimotor cortices and the insular cortex. The attention and control networks were not split into two fully separated, dedicated networks. Overall, in dogs, fronto-parietal networks and hubs were less dominant than in humans, with the cingulate gyrus playing a central role. The current manuscript provides the first attempt to map whole-brain functional networks in dogs via a model-based approach.

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Resting state networks of the canine brain under sevoflurane anaesthesia

Cited by 12 publications

References 60 publications

Increased resting state connectivity in the anterior default mode network of idiopathic epileptic dogs

Increased resting state connectivity in the anterior default mode network of idiopathic epileptic dogs

Systematic Review: Anaesthetic Protocols and Management as Confounders in Rodent Blood Oxygen Level Dependent Functional Magnetic Resonance Imaging (BOLD fMRI)–Part A: Effects of Changes in Physiological Parameters

Central nodes of canine functional brain networks are concentrated in the cingulate gyrus

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