Resting nailfold capillary blood flow in primary open-angle glaucoma

Cousins, Clara C; Chou, Jonathan; Greenstein, Scott H.; Brauner, Stacey C.; Shen, Lucy Q.; Turalba, Angela; Houlihan, Patricia; Ritch, Robert; Wiggs, Janey L.; Knepper, Paul A.; Pasquale, Louis R.

doi:10.1136/bjophthalmol-2018-311846

Cited by 22 publications

(22 citation statements)

References 23 publications

(17 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This degeneration may cause the aforementioned alterations in the peripheral vessels, including elastosis, oxidative stress, and vascular endothelial dysfunction, leading to vascular stiffness [9]. Similar results related to lower peripheral blood flow in patients with HFG and NC were reported by Cousins et al [49], who explored resting peripheral blood flow at the nailfold in patients with POAG (HTG and NTG), which was found to be significantly lower compared with that in normal controls.…”

Section: Discussionsupporting

confidence: 52%

Nailfold capillary morphology and platelet function in patients with exfoliative glaucoma

et al. 2019

View full text Add to dashboard Cite

Purpose The purpose of the present study was to evaluate the nailfold capillary morphological features in patients with exfoliative glaucoma (XFG) and compare them with those pertaining to primary open-angle glaucoma (POAG), normal controls and subjects with exfoliation syndrome (XFS). The second purpose was to investigate all parameters related to platelet function on the hemogram, including the platelet count (PLT), the mean platelet volume (MPV), platelet distribution width (PDW), and plateletcrit (PCT) in patients with XFG. These parameters were subsequently compared with those belonging to normal controls, POAG and XFS subjects. Methods This case control study involved 152 consecutive patients that were examined at the Glaucoma Department of Clinic for Eye Diseases, Clinical Centre of Serbia, as the referral center for glaucoma in Serbia, between June 2016 and December 2017. Results Regarding capillaroscopic characteristics, statistically significant difference was found in capillary diameter and tortuosity between the XFG and POAG group (p = 0.050 and p = 0.035) and the XFG and NC group (p = 0.003 and p = 0.044), as well as in the distribution of capillary loops and avascular zones between the XFG and NC group (p = 0.014 and p = 0.004). The subjects with XFG had lower PLT values compared to POAG patients (p = 0.022). Conclusions In conclusion, to the best of our knowledge, this study marks the first attempt to evaluate capillary morphology as well as to investigate all parameters related to platelet function on the hemogram, in patients with newly diagnosed XFG. Our findings revealed nailfold capillary morphological vascular changes in XFG patients. The subjects with XFG had lower PLT values and a higher MPV serum parameter compared to normal controls and patients with POAG. Further research in this field should therefore aim to evaluate the consequences of the aforementioned microvascular abnormalities in patients with XFG.

show abstract

Section: Discussionsupporting

confidence: 52%

Nailfold capillary morphology and platelet function in patients with exfoliative glaucoma

et al. 2019

View full text Add to dashboard Cite

show abstract

“…We customized a high throughput PCR-based strategy to identify differentially expressed miRNAs relevant to glaucoma, then applied this tool to test AqH and plasma from glaucoma patients and cataract controls. We focused our analysis on plasma samples; in addition to its ease of collection (relative to AqH, which requires an elective procedure), at least some glaucoma endophenotypes are associated with vascular dysfunction,19,20 supporting the potential relevance for plasma biomarkers as a diagnostic tool for glaucoma 21–23. Moreover, our complete dataset included matched AqH samples for each subject, allowing comparisons between the two sample types and further investigation of ocular miRNAs in glaucoma etiology.…”

mentioning

confidence: 99%

Identification of Candidate miRNA Biomarkers for Glaucoma

Hindle

Thoonen

Jasien

et al. 2019

Invest. Ophthalmol. Vis. Sci.

Self Cite

View full text Add to dashboard Cite

PurposeGlaucoma, a leading cause of blindness worldwide, often remains undetected until irreversible vision loss has occurred. Treatments focus on lowering intraocular pressure (IOP), the only modifiable and readily measurable risk factor. However, IOP can vary and does not always predict disease progression. MicroRNAs (miRNAs) are promising biomarkers. They are abundant and stable in biological fluids, including plasma and aqueous humor (AqH). We aimed to identify differentially expressed miRNAs in AqH and plasma from glaucoma, exfoliation syndrome (XFS), and control subjects.MethodsPlasma and AqH from two ethnic cohorts were harvested from glaucoma or XFS (often associated with glaucoma, n = 33) and control (n = 31) patients undergoing elective surgery. A custom miRNA array measured 372 miRNAs. Molecular target prediction and pathway analysis were performed with Ingenuity Pathway Analysis (IPA) and DIANA bioinformatical tools.ResultsLevels of miRNAs in plasma, a readily accessible biomarker source, correlated with miRNA levels in AqH. Twenty circulating miRNAs were at least 1.5-fold higher in glaucoma or XFS patients than in controls across two ethnic cohorts: miR-4667-5p (P = 4.1 × 10−5), miR-99b-3p (P = 4.8 × 10−5), miR-637 (P = 5.1 × 10−5), miR-4490 (P = 5.7 × 10−5), miR-1253 (P = 6.0 × 10−5), miR-3190-3p (P = 3.1 × 10−4), miR-3173-3p (P = 0.001), miR-608 (P = 0.001), miR-4725-3p (P = 0.002), miR-4448 (P = 0.002), and miR-323b-5p (P = 0.002), miR-4538 (P = 0.003), miR-3913-3p (P = 0.003), miR-3159 (P = 0.003), miR-4663 (P = 0.003), miR-4767 (P = 0.003), miR-4724-5p (P = 0.003), miR-1306-5p (P = 0.003), miR-181b-3p (P = 0.004), and miR-433-3p (P = 0.004). miR-637, miR-1306-5p, and miR-3159, in combination, allowed discrimination between glaucoma patients and control subjects (AUC = 0.91 ± 0.008, sensitivity 85.0%, specificity 87.5%).ConclusionsThese results identify specific miRNAs as potential biomarkers and provide insight into the molecular processes underlying glaucoma.

show abstract

“…37 However, the minimal decrease is in contrast with another study that found significantly decreased blood flow in both patients with HTG and NTG compared with controls. 36 This difference may be the result of a higher percentage of white participants compared with controls (72% in our study vs 91.5% in the prior study 41 ), different methods (using blood flow only in the fourth digit in our study vs using both the fourth and fifth digits in the previous study), and subjectivity in NFC blood flow analysis between the 2 investigations. A recent study found that patients with NTG showed more signs of vascular dysfunction compared with those with HTG, further demonstrating the possible differences in the pathophysiologic mechanisms between the 2 variants.…”

Section: Discussionmentioning

confidence: 54%

“…Lower mean (SD) nailfold capillary blood flow in patients with NTG compared with controls (40.1 [16.6] vs 70.9 [52.4] pL/s; P = .02) in our study is consistent with that of previous studies. 36,37 Systemic and IOP-independent vascular involvement in NTG may be noted in the context of Flammer syndrome, a primary vascular dysregulation entity with multiple manifestations that play a part in the pathogenesis of NTG.…”

Section: Discussionmentioning

confidence: 99%

“…Details of blood flow analysis and how plasma gap movement in capillaries is an acceptable estimate of capillary blood movement have been previously described. 36,45 Nine capillaries at the fourth digit of each participant with well-visualized plasma gap movement were chosen for grading. A single experienced grader (S.P.)…”

Section: Video-nailfold Capillaroscopy Proceduresmentioning

confidence: 99%

See 1 more Smart Citation

Nailfold Capillaroscopy of Resting Peripheral Blood Flow in Exfoliation Glaucoma and Primary Open-Angle Glaucoma

et al. 2019

Self Cite

View full text Add to dashboard Cite

laucoma is a progressive neurodegenerative ocular disease that causes visual field loss owing to damage of the retinal ganglion cells. The most common type of glaucoma in the United States, primary open-angle glaucoma (POAG), 1 has been classified into normal-tension (NTG) and high-tension glaucoma (HTG) based on the cutoff point for the statistical reference range of intraocular pressure (IOP). 2 High-tension glaucoma is associated with various risk factors, such as older age, high IOP, African descent, and positive family history. 3 Exfoliation syndrome (XFS), an age-related systemic condition, is the most common recognizable cause of open-angle glaucoma worldwide, comprising most cases in some countries. 4 Exfoliation syndrome is characterized by the production, deposition, and progressive accumulation of a white fibrillogranular extracellular material (exfoliation material) throughout the anterior segment, most prominently on the anterior lens surface, on the zonules with their resultant weakness, and on the pupillary border. Exfoliation material is also found in posterior segment vessels, including the vortex veins, posterior ciliary arteries, and central retinal vessels. It is observed microscopically in numerous organs, including the skin, heart, lungs, and kidney, primarily in the connective tissue. 5-7 In addition to obstructing fluid pathways, exfoliation material accumulation affects vascular endothelial cells as well as contractile pericytes, which wrap IMPORTANCE Systemic blood flow alterations have been described using video nailfold capillaroscopy (NFC) in high-tension glaucoma (HTG) and normal-tension glaucoma (NTG) variants of primary open-angle glaucoma (POAG). To date, no previous studies have explored alterations in nailfold capillary blood flow in exfoliation glaucoma (XFG). OBJECTIVE To investigate the measure of peripheral blood flow as a surrogate marker of systemic vascular involvement in patients with XFG, HTG, and NTG, as well as in individuals serving as controls, using NFC.

show abstract

Resting nailfold capillary blood flow in primary open-angle glaucoma

Abstract: Reduced resting nailfold capillary blood flow is present in POAG independent of covariates such as blood pressure, pulse and IOP.

Cited by 22 publications

References 23 publications

Nailfold capillary morphology and platelet function in patients with exfoliative glaucoma

Nailfold capillary morphology and platelet function in patients with exfoliative glaucoma

Identification of Candidate miRNA Biomarkers for Glaucoma

Nailfold Capillaroscopy of Resting Peripheral Blood Flow in Exfoliation Glaucoma and Primary Open-Angle Glaucoma

Contact Info

Product

Resources

About