REST levels affect the functional expression of voltage dependent calcium channels and the migratory activity in immortalized GnRH neurons

Antoniotti, Susanna; Torriano, Simona; Luganini, Anna; D’Alessandro, Rosalba; Lovisolo, Davide

doi:10.1016/j.neulet.2016.06.050

Cited by 4 publications

(3 citation statements)

References 26 publications

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“…Various Ca 2+ signaling was repressed by REST differentially [106]. The functional expression of P/Q-type channels was increased by REST silencing in GN11 neuron cells [107], while the promotor activity of N-type Ca 2+ channel alpha 1B was not influenced by REST in NS20Y cells [108]. Recently, REST was found to control spatial K buffering in primary cortical astrocytes, impairing neuronal activity [109].…”

Section: Neuronal Transmission and Synaptic Plasticitymentioning

confidence: 99%

REST Is Not Resting: REST/NRSF in Health and Disease

Jin,

Liu,

et al. 2023

Biomolecules

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Chromatin modifications play a crucial role in the regulation of gene expression. The repressor element-1 (RE1) silencing transcription factor (REST), also known as neuron-restrictive silencer factor (NRSF) and X2 box repressor (XBR), was found to regulate gene transcription by binding to chromatin and recruiting chromatin-modifying enzymes. Earlier studies revealed that REST plays an important role in the development and disease of the nervous system, mainly by repressing the transcription of neuron-specific genes. Subsequently, REST was found to be critical in other tissues, such as the heart, pancreas, skin, eye, and vascular. Dysregulation of REST was also found in nervous and non-nervous system cancers. In parallel, multiple strategies to target REST have been developed. In this paper, we provide a comprehensive summary of the research progress made over the past 28 years since the discovery of REST, encompassing both physiological and pathological aspects. These insights into the effects and mechanisms of REST contribute to an in-depth understanding of the transcriptional regulatory mechanisms of genes and their roles in the development and progression of disease, with a view to discovering potential therapeutic targets and intervention strategies for various related diseases.

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Section: Neuronal Transmission and Synaptic Plasticitymentioning

confidence: 99%

REST Is Not Resting: REST/NRSF in Health and Disease

Jin,

Liu,

et al. 2023

Biomolecules

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“…Indeed, consistent with their trajectories, the microtubuleassociated protein stathmin (Stmn2) and the repressor element-1 silencing transcription factor (Rest) promote migration in immortalized GnRH neurons. 24,41 The trajectory analysis of GnRH-negative cells revealed T-box transcription factor 3 (Tbx3) as a gene with high expression in the early stages in the nasal compartment (Fig. 3f).…”

Section: Spatiotemporal Expression Trajectories Define Gnrh Neuron De...mentioning

confidence: 99%

Transcriptomic profiling of murine GnRH neurons reveals developmental trajectories linked to human reproduction

Zouaghi

Alpern

Gardeux

et al. 2023

Preprint

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Gonadotropin-releasing hormone (GnRH) neurons play a crucial role in human reproduction and are associated with a spectrum of conditions. However, the underlying biological mechanisms remain elusive due to their small number and sparse distribution. We performed transcriptomic profiling of GnRH neurons during mouse embryonic development, revealing their molecular identity and gene expression dynamics. Our findings show that GnRH neurons undergo a profound transcriptional shift as they migrate from the nose to the brain and that distinct expression trajectories are associated with critical biological processes, including cell migration, neuronal projections, and synapse formation. Cell-to-cell communication analysis revealed timely and spatially restricted modulation of signaling pathways involving known molecules, such as Semaphorins and Plexins, and novel candidates, such as Neurexins and Endothelins. Using GWAS genes linked to human reproductive onset, we found a specific association with GnRH neuron trajectories rising in late developmental stages and involved in neuron maturation and connectivity. Finally, analysis of the genetic burden in a large cohort of patients with congenital GnRH deficiency revealed specific GnRH neuron trajectories with a significant mutation load compared to controls. In conclusion, this study revealed the gene expression dynamics underlying GnRH neuron embryonic development and provides novel insights linking GnRH neuron biology to human reproduction.

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“…Indeed, a recent study has shown REST co-expressed in GnRH neurons, which increases the functional expression of calcium channels and reduces their migratory potential (Antoniotti et al, 2016). The chemical nature of REST-positive neurons in the pre-optic and midbrain areas needs to be identified.…”

Section: Expression Of Rest In Glial Cells and Neuronsmentioning

confidence: 99%

Expression of Repressor Element 1 Silencing Transcription Factor (REST) in Serotonin Neurons in the Adult Male Nile Tilapia (Oreochromis niloticus)

2021

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Repressor element-1 silencing transcription factor (REST) is highly expressed in the dorsal raphe where serotonin (5-hydroxytryptamine, 5-HT) neurons are located. REST works as a transcription factor for the 5-HT receptor and tryptophan hydroxylase two-gene expression. We hypothesized that REST is co-expressed in 5-HT neurons, which, if demonstrated, would be useful to understand the mechanism of 5-HT dysfunction-related disorders such as negative emotions and depression. Therefore, the present study was designed to examine the expression of the REST gene in the brain (forebrain, midbrain, and hindbrain) of adult male Nile tilapia (Oreochromis niloticus) using rt-PCR. Besides, using immunocytochemistry, co-localization of the REST gene was examined in 5-HT neurons and with neuronal-/glial-cell markers. We found a high expression of the REST gene in the midbrain region of the dorsal raphe, an area of 5-HT neurons. Double-label immunocytochemistry showed neuron-specific expression of REST co-localized in 5-HT neurons in the dorsal and ventral parts of the periventricular pretectal nucleus, paraventricular organ, and dorsal and medial raphe nucleus. Since midbrain 5-HT neurons express REST, we speculate that REST may control 5-HT neuronal activity related to negative emotions, including depression.

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REST levels affect the functional expression of voltage dependent calcium channels and the migratory activity in immortalized GnRH neurons

Cited by 4 publications

References 26 publications

REST Is Not Resting: REST/NRSF in Health and Disease

REST Is Not Resting: REST/NRSF in Health and Disease

Transcriptomic profiling of murine GnRH neurons reveals developmental trajectories linked to human reproduction

Expression of Repressor Element 1 Silencing Transcription Factor (REST) in Serotonin Neurons in the Adult Male Nile Tilapia (Oreochromis niloticus)

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