Responsiveness to phytoestrogens in primary human osteoblasts is modulated differentially by a “less-calcemic” analog of 1,25 dihydroxyvitamin D3: JK 1624F2-2 (JKF)

Mutual interaction of special phytoestrogenic compounds, their synthetic carboxy-derivatives and the less-calcemic vitamin D analog activities in human derived female cultured osteoblasts

“…This indicates that membranal processes are not involved in CK stimulation by estrogenic compounds tested in this study or others (Fig. 6) [19,21,22].…”

“…1 and 2) [22,29]. This might be a defense mechanism in the patho-physiological conditions of absence of vitamin D in the body.…”

25 hydroxy-vitamin D3-1α hydroxylase expression and activity in cultured human osteoblasts and their modulation by parathyroid hormone, estrogenic compounds and dihydrotestosterone

“…The mechanism of these changes and their importance is not clear. The effect of the locally formed 1,25D might be involved in the modulatory effects of vitamin D on the hormonal responses of hOB to the different estrogenic compounds which might be due to the changes in the expression of estrogen receptors, which were reported to be changed with external vitamin D treatments as described previously [41,42]. This might be a defence mechanism in the patho-physiological conditions of absence of vitamin D in the body.…”

Biogerontology research in Israel

“…Further studies are addressing the expression of the estrogen receptor (ER) in the Ob cells. Expression of ERa mRNA was higher whereas ERb mRNA was lower in pre-vs. post-menopausal Ob, respectively, with no significant age difference in intracellular and membranal estrogen binding activities (Somjen et al 2006b). ERa mRNA expression was stimulated by E2 in pre-and inhibited in post-menopausal Ob, while ERb mRNA expression was inhibited in samples of both age stages, yet, it was greater in the post-menopausal cells.…”