Responsiveness to ATP with an increase in intracellular free Ca2+ is not a distinctive feature of calbindin-D28 immunoreactive neurons in myenteric ganglia

Christofi, Fievos L.; Guan, Zhen; Lucas, Jen Hill; Rosenberg-Schaffer, Lisa J.; Stokes, Bradford T.

doi:10.1016/0006-8993(96)00280-6

Cited by 12 publications

(2 citation statements)

References 25 publications

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“…Many calbindin-positive neurons, which were shown to be mostly AH-type neurons (10), were immunopositive for Na ϩ channels. These conclusions are supported by Ca 2ϩ -imaging studies showing that both neuron types have voltage-activated Ca 2ϩ channels (5,20). Thus the distinction between AH-and S-type neurons on the basis of the nature of the action potentials is not as sharp as thought previously and is more a matter of degree.…”

Section: Discussionmentioning

confidence: 57%

Patch-clamp study of neurons and glial cells in isolated myenteric ganglia

Hanani

Francke

Härtig

et al. 2000

American Journal of Physiology-Gastrointestinal and Liver Physi

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Most of the physiological information on the enteric nervous system has been obtained from studies on preparations of the myenteric ganglia attached to the longitudinal muscle layer. This preparation has a number of disadvantages, e.g., the inability to make patch-clamp recordings and the occurrence of muscle movements. To overcome these limitations we used isolated myenteric ganglia from the guinea pig small intestine. In this preparation movement was eliminated because muscle was completely absent, gigaseals were obtained, and whole cell recordings were made from neurons and glial cells. The morphological identity of cells was verified by injecting a fluorescent dye by micropipette. Neurons displayed voltage-gated inactivating inward Na(+) and Ca(2+) currents as well as delayed-rectifier K(+) currents. Immunohistochemical staining confirmed that most neurons have Na(+) channels. Neurons responded to GABA, indicating that membrane receptors were retained. Glial cells displayed hyperpolarization-induced K(+) inward currents and depolarization-induced K(+) outward currents. Glia showed large "passive" currents that were suppressed by octanol, consistent with coupling by gap junctions among these cells. These results demonstrate the advantages of isolated ganglia for studying myenteric neurons and glial cells.

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Section: Discussionmentioning

confidence: 57%

Patch-clamp study of neurons and glial cells in isolated myenteric ganglia

Hanani

Francke

Härtig

et al. 2000

American Journal of Physiology-Gastrointestinal and Liver Physi

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“…Only one study (19) reports simultaneous recordings of electrical responses in different myenteric neurons. In recent years, new techniques have been developed to visualize neuronal activation either by voltage-sensitive dyes (21)(22)(23) or calcium indicators (7,9,27,39,(41)(42)(43)(44)(45). Electrical stimulation of interneuronal fibers of cultured myenteric neurons induced a rise in intracellular calcium concentration ([Ca 2ϩ ] i ), which can be monitored by confocal recording of fluorescence changes in high-affinity calcium indicators, such as fluo 3.…”

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confidence: 99%

Electrical stimulation reveals complex neuronal input and activation patterns in single myenteric guinea pig ganglia

Bisschops

Berghe

Bellon

et al. 2003

American Journal of Physiology-Gastrointestinal and Liver Physi

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The myenteric plexus plays a key role in the control of gastrointestinal motility. We used confocal calcium imaging to study responses to electrical train stimulation (ETS) of interganglionic fiber tracts in entire myenteric ganglia of the guinea pig small intestine. ETS induced calcium transients in a subset of neurons: 52.2% responded to oral ETS, 65.4% to aboral ETS, and 71.7% to simultaneous oral and aboral ETS. A total of 41.3% of the neurons displayed convergence of oral and aboral ETS-induced responses. Responses could be reversibly blocked with TTX (10(-)6 M), demonstrating involvement of neuronal conduction, and by removal of extracellular calcium. omega-Conotoxin (5 x 10(-7) M) blocked the majority of responses and reduced the amplitude of residual responses by 45%, indicating the involvement of N-type calcium channels. Staining for calbindin and calretinin did not reveal different response patterns in these immunohistochemically identified neurons. We conclude that, at least for ETS close to a ganglion, confocal calcium imaging reveals complex oral and aboral input to individual myenteric neurons rather than a polarization in spread of activity.

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Peripheral Nervous System

Burnstock¹,

Verkhratsky

2012

Purinergic Signalling and the Nervous System

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Responsiveness to ATP with an increase in intracellular free Ca2+ is not a distinctive feature of calbindin-D28 immunoreactive neurons in myenteric ganglia

Cited by 12 publications

References 25 publications

Patch-clamp study of neurons and glial cells in isolated myenteric ganglia

Patch-clamp study of neurons and glial cells in isolated myenteric ganglia

Electrical stimulation reveals complex neuronal input and activation patterns in single myenteric guinea pig ganglia

Peripheral Nervous System

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