Responsive hetero-organelle partition conferred fluorogenic sensing of mitochondrial depolarization

Abstract

A hetero-organelle partition and lysosome activable sensor was developed for fluorogenic detection of mitochondrial depolarization in autophagy and cell death.

Defining Cancer Cell Bioenergetic Profiles Using a Dual Organelle-Oriented Chemosensor Responsive to pH Values and Electropotential Changes

“…HeLa cells cultivated with varied doses of RC-AMI display relatively constant coumarin to rhodamine (CM/ROX) fluorescence ratios in the range of 1.5−2.0 (Figure 3). In line with reported studies, 11 RC-TPP selectively accumulates in mitochondria with high CM/ROX fluorescence ratios around 25, whereas rhodamine−coumarin (RC), lacking an AMI moiety, is exclusively located in lysosomes (Figure 3C; Supporting Information, Figures S1 and S2). These findings validate the use of AMI as a ΔΨ m -responsive entity with attenuated affinity as compared to TPP, and feasibility of AMIintegrated lysosomal sensors for simultaneous partition in mitochondria and lysosomes.…”

“…Mitochondria and lysosomes are critical regulators of cell bioenergetics. We recently reported the detection of mitochondrial depolarization with a triphenylphosphonium (TPP)-conjugated diad of rhodamine–lactam/coumarin (RC-TPP), which selectively accumulates in mitochondria and relocates into lysosomes upon loss of ΔΨ m . Cationic TPP moiety efficiently ferries various cargoes into mitochondria. − We envisioned that integration of a lysosomal sensor with a ΔΨ m -responsive entity of attenuated affinity relative to TPP might afford a dual organelle-partitioned sensor capable of simultaneously reporting ΔΨ m and lysosomal pH.…”

Multicolor monitoring of cellular organelles by single wavelength excitation to visualize the mitophagy process

“…To investigate the mitophagy process, fluorescent probes that can stain mitochondria and lysosomes have been tested. 5 – 7 Among all the fluorescent moieties, organic dyes are the most promising 8 , 9 because of their non-invasiveness and small size, although they share the drawbacks of having small Stokes shifts, being easily photobleached 10 and undergoing facile aggregation-caused quenching (ACQ). 11 In addition, two wavelengths are often needed to excite both dyes to emit different colors.…”

Decrease of Protein Vicinal Dithiols in Parkinsonism Disclosed by a Monoarsenical Fluorescent Probe

“…However, these traditional biochemical methods are not compatible with real-time and in situ analysis of VDPs. In contrast, fluorescence imaging, owing to its good biocompatibility and temporal-spatial resolution, − has gained increasing interests in biomedical studies. To date, several fluorescent probes of VDPs have been developed by different groups .…”