Responsive disassembly of nucleic acid nanocomplex in cells for precision medicine

Branched DNA with multibranch-like anisotropic topology serves as a promising and powerful building block in constructing multifunctional-integrated nanomaterials in a programmable and controllable manner. Recently, a series of branched DNA-based functional nanomaterials were developed by elaborate molecular design. In this review, we focused on the construction of branched DNA-based nanostructures for biological and biomedical applications. First, the molecular design and synthesis method of branched DNA monomer were briefly described. Then, the construction strategies of branched DNA-based nanostructures were categorially discussed, including target-triggered polymerization, enzymatic extension and hybrid assembly. Finally, the biological and biomedical applications including diagnosis, therapeutics and protein engineering were summarized. We envision that the review will contribute to the further development of branched DNA-based nanomaterials with great application potential in the field of biomedicine, thus building a new bridge between material chemistry and biomedicine.

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Section: Diagnosismentioning

confidence: 99%

Construction of Branched DNA‐based Nanostructures for Diagnosis, Therapeutics and Protein Engineering

Dong

Yao

et al. 2022

Chemistry An Asian Journal

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“…[24] In 2021, we developed A549 lung cancer cell membranecamouflaged nanocomplex (nanocomplex@A549m) for smart drug delivery and gene therapy (Figure 5). [25] Specifically, the siRNA linker with sticky ends could assemble with Y-DNA to form branched-DNA/RNA. TA could further facilitate the assembly of branched-DNA/RNA and anchor A549 cell membrane on nanocomplex to form nanocomplex@A549m.…”

Section: Dna-ta Nanomaterialsmentioning

confidence: 99%

mentioning

confidence: 99%

Dynamic Transformation of DNA Nanostructures inside Living Cells

Ding

Lü

et al. 2022

ChemPlusChem

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DNA nanostructures, that show sequence programmable responsiveness to intracellular signals, have been explored as potential candidates of artificial dynamic functional structures in living cells. Recently, we developed a series of intracellular signals responding DNA nanostructures in living cells to perform a special mission. In this review, the developed DNA nanostructures and their dynamic transformation properties are summarized and discussed. The DNA nanostructures are generally categorized into DNA-inorganic nanomaterials and DNA-organic nanomaterials depending on the composition. At the end of this review, the challenges and prospects on instructing dynamic DNA nanostructures in living cells are discussed. We believe that this review will contribute to the further development of stimuli-responsive nanomaterials, which is of great potential in biomedical applications.

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“…Inspired by the camouflage mechanism of cancer cells against the immune system, the cell membrane coating strategy provided feasible means to manipulate the fates of original nanocarriers. Due to the structural affinity between TAs and proteins, we further coated DNA-polyphenol nanocomplex with the cell membrane (Figure 6B; Han et al, 2021b). The homotypic targeting capability derived from the membrane improved the blood circulation stability of nanocomplex and reduced the clearance by the reticular endothelial system.…”

Section: Dna-organic Nanomaterialsmentioning

confidence: 99%

DNA Functional Nanomaterials for Controlled Delivery of Nucleic Acid-Based Drugs

Lü

Zhu

2021

Front. Bioeng. Biotechnol.

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Nucleic acid-based drugs exhibited great potential in cancer therapeutics. However, the biological instability of nucleic acid-based drugs seriously hampered their clinical applications. Efficient in vivo delivery is the key to the clinical application of nucleic acid-based drugs. As a natural biological macromolecule, DNA has unique properties, such as excellent biocompatibility, molecular programmability, and precise assembly controllability. With the development of DNA nanotechnology, DNA nanomaterials have demonstrated significant advantages as delivery vectors of nucleic acid-based drugs by virtue of the inherent nucleic acid properties. In this study, the recent progress in the design of DNA-based nanomaterials for nucleic acid delivery is summarized. The DNA nanomaterials are categorized according to the components including pure DNA nanomaterials, DNA-inorganic hybrid nanomaterials, and DNA-organic hybrid nanomaterials. Representative applications of DNA nanomaterials in the controlled delivery of nucleic acid-based drugs are exemplified to show how DNA nanomaterials are rationally and exquisitely designed to address application issues in cancer therapy. At the end of this study, the challenges and future development of DNA nanomaterials are discussed.

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Responsive disassembly of nucleic acid nanocomplex in cells for precision medicine

Cited by 41 publications

References 55 publications

Construction of Branched DNA‐based Nanostructures for Diagnosis, Therapeutics and Protein Engineering

Construction of Branched DNA‐based Nanostructures for Diagnosis, Therapeutics and Protein Engineering

Dynamic Transformation of DNA Nanostructures inside Living Cells

DNA Functional Nanomaterials for Controlled Delivery of Nucleic Acid-Based Drugs

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