Responses to Intradermal Injections of Substance P in Psoriasis Patients with Pruritus

Amatya, Beni; Nordlind, Klas; Wahlgren, Carl-Fredrik

doi:10.1159/000270385

Cited by 30 publications

(26 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the underlying mechanisms by which these chemicals mediate itch are unclear. Substance P has been reported to produce itch in humans mainly by degranulating mast cells to release histamine (Hagermark et al, 1978; Amatya et al, 2010). A wheal and flare accompanied the itch evoked by intradermal injections of substance P in these studies.…”

Section: Discussionmentioning

confidence: 99%

BAM8–22 Peptide Produces Itch and Nociceptive Sensations in Humans Independent of Histamine Release

Sikand¹,

Dong²,

LaMotte³

2011

J. Neurosci.

164

151

View full text Add to dashboard Cite

Chronic itch accompanying many dermatological, neurological and systemic diseases is unresponsive to antihistamines. Our knowledge of endogenous chemicals that evoke histamine-independent itch and their molecular targets is very limited. Recently it was demonstrated in behavioral and cellular experiments that bovine adrenal medulla 8–22 peptide (BAM8–22), a proteolytically cleaved product of proenkephalin A, is a potent activator of Mas-related G protein-coupled receptors (Mrgprs), MrgprC11 and hMrgprX1, and induces scratching in mice in a Mrgpr-dependent manner. To study the sensory qualities that BAM8–22 evokes in humans we tested the volar forearm of 15 healthy volunteers with heat-inactivated cowhage spicules previously soaked in the peptide. BAM8–22 produced itch in each subject, usually accompanied by sensations of pricking/stinging and burning. The sensations were occasionally accompanied by one or more mechanically evoked dysesthesias, namely alloknesis, hyperknesis, and hyperalgesia, but no wheal or neurogenic flare in the skin surrounding the application site. The inactive truncated peptide BAM8–18 produced weak or no sensations. Pretreatment of the tested skin with an antihistamine cream (doxepin) inhibited the histamine-induced sensations, dysesthesias and skin reactions but not the sensations and dysesthesias evoked by BAM8–22. We show that BAM8–22 produces itch and nociceptive sensations in humans in a histamine-independent manner. Thus, BAM8–22 may be an endogenous itch mediator that activates, in humans, MrgprX1, a novel target for potential anti-itch treatments.

show abstract

Section: Discussionmentioning

confidence: 99%

BAM8–22 Peptide Produces Itch and Nociceptive Sensations in Humans Independent of Histamine Release

Sikand¹,

Dong²,

LaMotte³

2011

J. Neurosci.

164

151

View full text Add to dashboard Cite

show abstract

“…We considered 5 min to be the most appropriate time period, based on earlier studies (e.g. 8,11,19), and on the results of a pilot study in the present investigation, which showed fairly constant values for wheal, while flare increased slightly over time (0-15 min). The maximum perpendicular diameters for the flare were measured in mm and the surface calculated in mm^ (20), while, for the wheal the maximum diameter in mm was recorded.…”

Section: Recording Experimental Pruritusmentioning

confidence: 95%

Pruritic and Vascular Responses Induced by Serotonin in Patients with Atopic Dermatitis and in Healthy Controls

Rausl¹,

Nordlind²,

Wahlgren³

2013

Acta Derm Venerol

View full text Add to dashboard Cite

Atopic dermatitis (AD) is a chronic inflammatory skin disease with often severe itch. The aim of this study was to determine the pruritogenic and vascular effect of serotonin (5-hydroxytryptamine; 5-HT) in patients with AD and in healthy controls. A 50 µg dose of 5-HT was injected intradermally into non-lesional skin of 25 patients with AD and 25 healthy control individuals, and the effect compared with 0.2 µg histamine as a positive control, and buffer as a negative control. Pruritus was recorded by the subjects, using a computerized visual analogue scale, while flare and wheal were recorded by the investigator. There was no qualitative or quantitative difference in 5-HT-induced itch between patients and control subjects, or between males and females. However, reduced flare and wheal were found in the patient group for 5-HT. There were no correlations between clinical findings (i.e. eczema severity, clinical pruritus) and recorded experimental itch, or flare or wheal responses for 5-HT, in the patients with AD. In both groups a shorter itch latency was found for 5-HT compared with histamine. Through the use of intradermal injections, making it possible to calculate the dose of substance delivered, a lower vascular response to 5-HT was shown in patients with AD compared with healthy controls. In addition to confirming a pruritogenic role of 5-HT in both patients with AD and healthy controls, we found a shorter itch latency for 5-HT compared with histamine in both groups. The short itch latency time may indicate a direct effect of 5-HT on itch receptors.

show abstract

“…Administration of SP elicits scratching in both mice and humans through activation of the NK1R [166, 167]. Intrathecal injection of SP also rescues the loss of histamine-induced scratching in African naked mole rats [168].…”

Section: The Molecular Basis Of Pain and Itchmentioning

confidence: 99%

Molecular and cellular mechanisms that initiate pain and itch

Luo

Feng

Liu

et al. 2015

Cell. Mol. Life Sci.

View full text Add to dashboard Cite

Somatosensory neurons mediate our sense of touch. They are critically involved in transducing pain and itch sensations under physiological and pathological conditions, along with other skin resident cells. Tissue damage and inflammation can produce a localized or systemic sensitization of our senses of pain and itch, which can facilitate our detection of threats in the environment. Although acute pain and itch protect us from further damage, persistent pain and itch are debilitating. Recent exciting discoveries have significantly advanced our knowledge of the roles of membrane-bound G protein-coupled receptors and ion channels in the encoding of information leading to pain and itch sensations. This review focuses on molecular and cellular events that are important in early stages of the biological processing that culminates in our senses of pain and itch.

show abstract

Responses to Intradermal Injections of Substance P in Psoriasis Patients with Pruritus

Cited by 30 publications

References 48 publications

BAM8–22 Peptide Produces Itch and Nociceptive Sensations in Humans Independent of Histamine Release

BAM8–22 Peptide Produces Itch and Nociceptive Sensations in Humans Independent of Histamine Release

Pruritic and Vascular Responses Induced by Serotonin in Patients with Atopic Dermatitis and in Healthy Controls

Molecular and cellular mechanisms that initiate pain and itch

Contact Info

Product

Resources

About