Responses of Yeast Cells to Heat Applied Alone or Combined with Gamma-rays

Петин, В.Г.; Berdnikova, I P

doi:10.1080/09553008114550361

Cited by 11 publications

(4 citation statements)

References 11 publications

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“…In some cases, for practical and theoretical reasons, it is of great importance to provide information concerning the interaction. In this paper, to calculate the synergistic e ect, one used the synergistic enhancement ratio (k) de®ned as the ratio of the exposure time expected for an independent e ect of both modalities to that observed from the experimental survival curve for the simultaneous action of hyperthermia and another inactivating factor 15 . In this paper, this parameter was calculated at 10% survival, kˆt 3 =t 4 for sigmoidal survival curves (®gures 1(a±c)).…”

Section: Resultsmentioning

confidence: 99%

Some general regularities of synergistic interaction of hyperthermia with various physical and chemical inactivating agents

Петин

Kim

Zhurakovskaya

et al. 2002

International Journal of Hyperthermia

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Various cellular systems have been analysed for synergistic interactions of the simultaneous action of hyperthermia with physical agents including ionizing radiation, ultraviolet light, and ultrasound, and with chemical agents including thio-TEPA and cis-DDP. Some general rules of synergistic interaction were revealed. First, for every constant rate or intensity of exposure to physical factors or concentration of chemical agents, synergy can be observed only within a certain temperature range that is different for various cellular systems. Secondly, within this range, there is a specific temperature that maximizes the synergistic effect. Any deviation of temperature from the optimal one results in a reduction in synergy. Thirdly, the rate of exposure to physical agents or the concentration of chemical agents strongly influences the synergy; i.e. as the dose rate or concentration is reduced, the temperature for maximum synergism decreases. Possible interpretations of the mechanisms responsible for these relationships of synergy are discussed.

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Section: Resultsmentioning

confidence: 99%

Some general regularities of synergistic interaction of hyperthermia with various physical and chemical inactivating agents

Петин

Kim

Zhurakovskaya

et al. 2002

International Journal of Hyperthermia

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“…Arrhenius plots for yeast cells of S. cerevisiae showed a distant break of the inactivation constant at 52.5 °C . Herein, all of the yeasts were dead under 60 °C for 30 min.…”

Section: Resultsmentioning

confidence: 79%

“…Arrhenius plots for yeast cells of S. cerevisiae showed a distant break of the inactivation constant at 52.5 °C. 30 Herein, all of the yeasts were dead under 60 °C for 30 min. The polymer is considered as a thermal insulating material to hinder the thermal transfer.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Synthesis of a Removable Cytoprotective Exoskeleton by Tea Polyphenol Complexes for Living Cell Encapsulation

Chen

Yang

et al. 2021

ACS Biomater. Sci. Eng.

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Cell encapsulation is a chemical tool for endowing living cells with exogenous properties and enhancing their in vitro tolerance against lethal factors, which has shown promising prospects and potential applications in many fields such as cell transplantation, drug delivery, and tissue engineering. One-pot precipitation of a polyphenol–metal complex on cells protects cells from UV irradiation and lytic enzymes. However, the involvement of metal ions brings side effects on cell viability and growth. Moreover, an external removal agent is needed for cell division and growth. Herein, a polymer shell composed of hydrogen bonded constituents without affecting cell viability and growth by the precipitation of tea polyphenol and polyvinyl pyrrolidone is reported. The formation of the polymer shell was verified by the Au nanoparticle’s laser scanning confocal reflectance and quartz crystal microbalance measurement. The thickness of the shell was managed by the concentration of the complex. When exposed to UV irradiation for 15 or 30 min, polymer-coating-protected Saccharomyces cerevisiae (yeast) had much higher cell viability than the native one. Exposed to a high temperature environment (60 °C), most of the coated yeasts survived in contrast to uncoated ones. For the cell division and growth curve, the polymer coating with various thicknesses had no difference to the native one, which indicated no suppression of cell growth and no external side effects involved. As applied to mammalian HeLa cells under UV irradiation for 15 min, the coated cells had an obvious higher cell viability than that of untreated ones. Therefore, the tea polyphenol–poly(vinylpyrrolidone) shell is a versatile tool for chemically controlling the external properties of cells without side effects on cell viability and growth.

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“…Into test tubes with sterile water (4.0 mL) preheated until the studied tem perature, there was added 0.5 mL of the chemical agent (for combination with hyperthermia) or 0.5 mL of distilled water (for action of hyperthermia alone) or To evaluate the synergic effect, we used coefficient of synergic enhancement k (Petin, 1977;Petin, Berd nikova, 1979, 1981 determined by the ratio of dura tion of action on theoretical curve of survival expected at independent addition of effects of the both agents (t 1 ) to the corresponding duration of effect estimated from the experimental curve of survival after the com bined action (t 2 ).…”

Section: Methodsmentioning

confidence: 99%

Manifestation of synergism under simultaneous action of hyperthermia and antitumor drugs on yeast cells

et al. 2014

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For the purpose of obtaining novel fundamental knowledge, there are studied regularities of man ifestation of synergism under simultaneous combined action of hyperthermia (47.5-60°C) and antitumor compounds (cyclophosphamide, cisplatin) on survival of yeast cells. To calculate the efficiency of synergic interaction, we used experimentally obtained dependences of cell survival on the duration of action after sep arate and simultaneous action of chemical agent and hyperthermia. A certain diapason of temperatures is shown, within which a synergic increase of the action of antitumor drug and hyperthermia occurs. Any devi ation of temperature from the optimal value leads to a decrease of synergism. A possible mechanism of the revealed regularity is discussed.

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Responses of Yeast Cells to Heat Applied Alone or Combined with Gamma-rays

Cited by 11 publications

References 11 publications

Some general regularities of synergistic interaction of hyperthermia with various physical and chemical inactivating agents

Some general regularities of synergistic interaction of hyperthermia with various physical and chemical inactivating agents

Synthesis of a Removable Cytoprotective Exoskeleton by Tea Polyphenol Complexes for Living Cell Encapsulation

Manifestation of synergism under simultaneous action of hyperthermia and antitumor drugs on yeast cells

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