Responses of two rice cultivars differing in seedling-stage nitrogen use efficiency to growth under low-nitrogen conditions

Shi, Wei; Xu, Wei; Li, Sumei; Zhao, Xue Qiang; Dong, Gang Qiang

doi:10.1007/s11104-009-0007-0

Cited by 79 publications

(60 citation statements)

References 41 publications

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“…Plants were harvested (including senescing leaves) then dried in an oven for 30 min at 105°C, then at 70°C to a constant weight and weighed. N concentration was determined using the Kjeldahl method (Shi et al, 2010;Han et al, 2015b); 0.2 g of dried samples were digested with H 2 SO 4 -H 2 O 2 in a 100-mL Kjeldahl digestion flask, after which N concentration was determined using a Foss Auto Analyzer Unit (Kjeldahl 8400).…”

Section: Acc Treatmentmentioning

confidence: 99%

Nitrogen Use Efficiency Is Mediated by Vacuolar Nitrate Sequestration Capacity in Roots of Brassica napus

et al. 2016

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Enhancing nitrogen use efficiency (NUE) in crop plants is an important breeding target to reduce excessive use of chemical fertilizers, with substantial benefits to farmers and the environment. In Arabidopsis (Arabidopsis thaliana), allocation of more NO 3 2 to shoots was associated with higher NUE; however, the commonality of this process across plant species have not been sufficiently studied. Two Brassica napus genotypes were identified with high and low NUE. We found that activities of V-ATPase and V-PPase, the two tonoplast proton-pumps, were significantly lower in roots of the high-NUE genotype (Xiangyou15) ] S:R ratios were significantly higher in Xiangyou15. BnNRT1.5 expression was higher in roots of Xiangyou15 compared with 814, while BnNRT1.8 expression was lower. In both B. napus treated with proton pump inhibitors or Arabidopsis mutants impaired in proton pump activity, vacuolar sequestration capacity (VSC) of NO 3 2 in roots substantially decreased. Expression of NRT1.5 was up-regulated, but NRT1.8 was down-regulated, driving greater NO 3 2 long-distance transport from roots to shoots. NUE in Arabidopsis mutants impaired in proton pumps was also significantly higher than in the wild type col-0. Taken together, these data suggest that decrease in VSC of NO 3 2 in roots will enhance transport to shoot and essentially contribute to higher NUE by promoting NO 3 2 allocation to aerial parts, likely through coordinated regulation of NRT1.5 and NRT1.8.

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Section: Acc Treatmentmentioning

confidence: 99%

Nitrogen Use Efficiency Is Mediated by Vacuolar Nitrate Sequestration Capacity in Roots of Brassica napus

et al. 2016

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“…2B). This increased expression at a higher dose may be associated with high levels of available nutrients in the environment because GS2 is the main glutathione synthetase isoform found in the leaves, and its overexpression is associated with maintaining photosynthetic capacity, photorespiration in plants and nitrogen assimilation (Shi et al, 2010). Furthermore, we did not observe differences upon comparing the doses with equal proportions of competing plants; however, the red rice monoculture showed its highest level of expression at the highest dose of nitrogen examined.…”

Section: Expression Of Genes Responsible For Nitrogen Assimilationmentioning

confidence: 53%

“…1). The AMT proteins in plants are located in the plasma membrane, which suggests that they play a role in ammonium uptake in plant cells (Shi et al, 2010). When we supplied nitrogen to plants at the appropriate dose (120 kg ha -1 ), expression of AMT mRNA increased in red rice plants.…”

Section: Expression Of Genes Responsible For Nitrogen Assimilationmentioning

confidence: 96%

Expression of genes in cultivated rice and weedy rice in competition

Nohato¹,

Benemann²,

Oliveira³

et al. 2016

Aust J Crop Sci

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The competition for resources such as light and nitrogen between red rice and cultivated rice can trigger responses in plants that interfere with growth and productivity as well as the expression of genes related to competition-induced stress. Due to its sensitivity, accuracy, and specificity, real-time reverse transcription PCR (RT-qPCR) is an important technique for analyzing differences in gene expression. In this study, we quantified the relative expression levels of genes involved in nitrogen assimilation (OsAMTs, OsGS2 and OsNADH-GOGAT2) and light capture (OsPIL1, OsCRY2 and OsCAB1) in cultivated and red rice in competition under different concentrations of nitrogen (0, 120 and 240 kg ha -1 of nitrogen). Interspecific competition in rice increased the expression of certain genes responsible for assimilating nitrogen (OsNADH-GOGAT2 and OsAMT3;1), and intraspecific competition in red rice also increased the expression of OsGS2. With the interspecific competition, both rice and red rice exhibited increased expression of genes responsible for capturing light, such as OsCRY2 and OsCAB1. With intraspecific competition, red rice showed increased OsPIL1 expression. Additionally, higher doses of nitrogen increased the expression of genes responsible for assimilating nitrogen and capturing light in both cultivated and wild rice species.

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“…This confirmed that the response of root to salt stress was more sensitive than that of leaf. Plant root was the primary Shi et al 2009). Glutamine synthetase (GS) primarily exists as two isozymes with different subcellular localisations: GS1 in the cytosol and GS2 in chloroplasts/plastids (Kusano et al 2011).…”

Section: Resultsmentioning

confidence: 99%

Effects of salt stress on ion balance and nitrogen metabolism in rice

Wang¹,

Wu²,

Zhou³

et al. 2012

Plant Soil Environ.

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The aim of this study was to test the effects of salt stress on nitrogen metabolism and ion balance in rice plants. The contents of inorganic ions, total amino acids, and NO 3 -in the stressed seedlings were then measured. The expressions of some critical genes involved in nitrogen metabolism were also assayed to test their roles in the regulation of nitrogen metabolism during adaptation of rice to salt stress. The results showed that when seedlings were subjected to salt stress for 4 h, in roots, salt stress strongly stimulated the accumulations of Na + and Cl -, and reduced K + content; however, in leaves, only at 5 days these changes were observed. This confirmed that the response of root to salt stress was more sensitive than that of leaf. When seedlings were subjected to salt stress for 4 h, salt stress strongly stimulated the expression of OsGS1;1, OsNADH-GOGAT, OsAS, OsGS1;3, OsGDH1, OsGDH2, OsGDH3 in both leaves and roots of rice, after this time point their expression decreased. Namely, at 5 days most of genes involved in NH 4 + assimilation were downregulated by salt stress, which might be the response to NO 3 -change. Salt stress did not reduce NO 3 -contents in both roots and leaves at 4 h, whereas at 5 days salt stress mightily decreased the NO 3 -contents. The deficiencies of NO 3 -in both roots and leaves can cause a large downregulation of OsNR1 and the subsequent reduction of NH 4 + production. This event might immediately induce the downregulations of the genes involved in NH 4 + assimilation.

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Responses of two rice cultivars differing in seedling-stage nitrogen use efficiency to growth under low-nitrogen conditions

Cited by 79 publications

References 41 publications

Nitrogen Use Efficiency Is Mediated by Vacuolar Nitrate Sequestration Capacity in Roots of Brassica napus

Nitrogen Use Efficiency Is Mediated by Vacuolar Nitrate Sequestration Capacity in Roots of Brassica napus

Expression of genes in cultivated rice and weedy rice in competition

Effects of salt stress on ion balance and nitrogen metabolism in rice

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