Responses of the macroalgae Hypnea musciformis after in vitro exposure to UV-B

Schmidt, Éder C.; Pereira, Beatriz; Santos, Rodrigo Weber dos; Gouveia, Claudiane; Costa, Giulia Burle; Faria, Gabriel Soares Mattar de; Scherner, Fernando; Horta, Paulo Antunes; Martins, Roberta de Paula; Latini, Alexandra; Ramlov, Fernanda; Maraschin, Marcelo; Bouzon, Zenilda L.

doi:10.1016/j.aquabot.2012.03.004

Cited by 52 publications

(34 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…fl oridanum (Schmidt et al, 2012a), and H . musciformis (Schmidt et al, 2012b). Meanwhile, in the present study, the amount of chl a was decreased in G .…”

Section: Fig3 Relative Electron Transport Rate (Retr) Of G Racilariacontrasting

confidence: 43%

“…domingensis (Schmidt et al, 2010c), Gelidium fl oridanum (Schmidt et al, 2012a), and H . musciformis (Schmidt et al, 2012b). UVBR is known to reduce the photosynthetic capacity (Gao and Xu, 2008), damage proteins of the PS II reaction centers and DNA (Zacher et al, 2007), and even induce the production of ROS (Lee and Shiu, Relative transport electron rate (rETR)…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have confi rmed that chlorophyll a (chl a ) concentrations change after exposure to UVBR (Huovinen et al, 2006;Sampath-Wiley et al, 2008;Heo and Jeon, 2009;Ju et al, 2011). Further, UVBR was found to alter the phycobiliprotein content in the macroalgae such as Gracilaria edulis (Eswaran et al, 2002), Gracilaria domingensis (Schmidt et al, 2010c) and Hypnea musciformis (Schmidt et al, 2012b). The decrease in photosynthetic activity during or after UV stress might result from the direct impairment to key components of the primary and secondary photosynthetic reactions, loss of photosynthetic pigments, as well as due to the reduced expression of genes involved in photosynthesis (Bischof et al, 2002;Bouzon et al, 2012;Polo et al, 2014).…”

Section: Introductionmentioning

confidence: 94%

“…Thus, elucidating the responses of G . lemaneiformis is essential to understand its ability to acclimate to UVBR stress (Schmidt et al, 2012b). In this case, G .…”

Section: Fig3 Relative Electron Transport Rate (Retr) Of G Racilariamentioning

confidence: 99%

See 3 more Smart Citations

Physiological responses of macroalga Gracilaria lemaneiformis (Rhodophyta) to UV-B radiation exposure

Zhu

Xiao

Wang

et al. 2014

Chin. J. Ocean. Limnol.

View full text Add to dashboard Cite

This paper aims to evaluate the effects of ultraviolet-B radiation (UVBR) on Gracilaria lemaneiformis , a commercial red macroalga and an important source of agar. To study the in-vitro effect of UVBR on G . lemaneiformis , this plant was cultivated and exposed to photosynthetically active radiation (PAR) at 40 μmol photons/(m 2 •s) and enhanced UVBR (0, 0.36, 0.72, 1.08, 1.44, and 1.80 kJ/(m 2 •d)) for 13 days. The samples were processed for histochemical analysis, and the growth rate, photosynthetic pigment contents, photosynthetic performance, reactive oxygen species levels, membrane permeability, malonyl dialdehyde contents and antioxidant capacity of G . lemaneiformis were investigated. After 13 days of exposure to PAR+UVBR, G . lemaneiformis showed photodamage and photoinhibition of photosynthetic pigments (chlorophyll a and phycoerythrin), leading to a decreased photosynthetic effi ciency. Further, there was a corresponding decrease in the relative growth rates and depigmentation and partial necrosis of the apical segments were noted after exposure to PAR+UVBR. Additionally, UVBR induced excess production of superoxide radicals and hydrogen peroxide, eliciting a marked cellular membrane damage and antioxidative response.

show abstract

“…fl oridanum (Schmidt et al, 2012a), and H . musciformis (Schmidt et al, 2012b). Meanwhile, in the present study, the amount of chl a was decreased in G .…”

Section: Fig3 Relative Electron Transport Rate (Retr) Of G Racilariacontrasting

confidence: 43%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 94%

“…Thus, elucidating the responses of G . lemaneiformis is essential to understand its ability to acclimate to UVBR stress (Schmidt et al, 2012b). In this case, G .…”

Section: Fig3 Relative Electron Transport Rate (Retr) Of G Racilariamentioning

confidence: 99%

See 2 more Smart Citations

Physiological responses of macroalga Gracilaria lemaneiformis (Rhodophyta) to UV-B radiation exposure

Zhu

Xiao

Wang

et al. 2014

Chin. J. Ocean. Limnol.

View full text Add to dashboard Cite

show abstract

“…Live algae (Y. poiteaui var., L. dendroidea, and L. translucida) were sectioned longitudinally with a razor blade, placed on glass cell chambers covered with coverslips (Corning Inc., Corning, NY, USA), and then immediately cellular responses of chloroplasts (e.g., CLs) exposed to different environmental conditions (Bouzon et al 2012, Gouveia et al 2013, Dos Santos et al 2014. On other hand, ultrastructure of CLs have been recognized as an important tool in red algae taxonomy and phylogeny (Kikuchi and Shin 2005, Müller et al 2010, Kushibiki et al 2012, even this characteristic being quite variable (Cole and Sheath 1980, Wilson et al 2002, Gillard et al 2008.…”

Section: Lsmmentioning

confidence: 99%

Chloroplasts morphology investigation with diverse microscopy approaches and inter-specific variation in Laurencia species (Rhodophyta)

Paradas¹,

Andrade²,

Salgado³

et al. 2015

ALGAE

View full text Add to dashboard Cite

The present study described with different microscopy approaches chloroplasts lobes in Laurencia sensu latu (Rhodophyta) species and found inter-specific differences among them. Chloroplasts were investigated using confocal laser scanning microscopy (LSM), transmission electron microscopy (TEM) and high resolution scanning electron microscopy (HRSEM). Using and TEM and HRSEM images we distinguished chloroplasts with lobes than chloroplasts without lobes in Yuzurua poiteaui var. gemmifera (Harvey) M. J. Wynne and Laurencia dendroidea J. Agardh cortical cells. The LSM images showed chloroplasts lobes (CLs) with different morphologies, varying from thicker and longer undulated projections in Y. poiteaui var. and L. dendroidea to very small and thin tubules as in Laurencia translucida Fujii & Cordeiro-Marino. The diameter and length of CLs from Y. poiteaui var. and L. dendroidea were significantly higher than L. translucida CLs (p < 0.01). Based on LSM observations, we suggest that lobes morphology has a taxonomic validity only to characterize L. translucida species.

show abstract

Improved metabolites of pharmaceutical ingredient grade Ginkgo biloba and the correlated proteomics analysis

Zheng

Zhang

et al. 2015

Proteomics

View full text Add to dashboard Cite

Ginkgo biloba is an attractive and traditional medicinal plant, and has been widely used as a phytomedicine in the prevention and treatment of cardiovascular and cerebrovascular diseases. Flavonoids and terpene lactones are the major bioactive components of Ginkgo, whereas the ginkgolic acids (GAs) with strong allergenic properties are strictly controlled. In this study, we tested the content of flavonoids and GAs under ultraviolet-B (UV-B) treatment and performed comparative proteomic analyses to determine the differential proteins that occur upon UV-B radiation. That might play a crucial role in producing flavonoids and GAs. Our phytochemical analyses demonstrated that UV-B irradiation significantly increased the content of active flavonoids, and decreased the content of toxic GAs. We conducted comparative proteomic analysis of both whole leaf and chloroplasts proteins. In total, 27 differential proteins in the whole leaf and 43 differential proteins in the chloroplast were positively identified and functionally annotated. The proteomic data suggested that enhanced UV-B radiation exposure activated antioxidants and stress-responsive proteins as well as reduced the rate of photosynthesis. We demonstrate that UV-B irradiation pharmaceutically improved the metabolic ingredients of Ginkgo, particularly in terms of reducing GAs. With high UV absorption properties, and antioxidant activities, the flavonoids were likely highly induced as protective molecules following UV-B irradiation.

show abstract

Responses of the macroalgae Hypnea musciformis after in vitro exposure to UV-B

Cited by 52 publications

References 35 publications

Physiological responses of macroalga Gracilaria lemaneiformis (Rhodophyta) to UV-B radiation exposure

Physiological responses of macroalga Gracilaria lemaneiformis (Rhodophyta) to UV-B radiation exposure

Chloroplasts morphology investigation with diverse microscopy approaches and inter-specific variation in Laurencia species (Rhodophyta)

Improved metabolites of pharmaceutical ingredient grade Ginkgo biloba and the correlated proteomics analysis

Contact Info

Product

Resources

About