Responses of the cutworm Spodoptera litura (Lepidoptera: Noctuidae) to two Bt corn hybrids expressing Cry1Ab

Yinghua, Shu; Du, Yuguo; Jin, Chen; Jiaxi, Wei; Wang, Jianwu

doi:10.1038/srep41577

Cited by 26 publications

(13 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…nubilalis , Yao et al [ 29 ] reported an overexpression of one CAT gene after feeding with Cry1Ab toxin. Furthermore, in another lepidopteran species, Spodoptera litura higher CAT activity was measured when larvae were fed on kernels of two Bt maize hybrids expressing Cry1Ab [ 30 ]. Since many carotenoids can directly scavenge O2 – in the cell [ 31 ], carotenoids can exhibit competitive relations with CAT, neutralizing the H 2 O 2 generated by O2 – dismutation.…”

Section: Discussionmentioning

confidence: 99%

Carotenoids moderate the effectiveness of a Bt gene against the European corn borer, Ostrinia nubilalis

et al. 2018

View full text Add to dashboard Cite

We assessed the effectiveness of a biofortified maize line (4BtxHC) which accumulates high levels of antioxidant carotenoids that also expressed the insecticidal Cry1Ac Bacillus thuringiensis (Bt) gene against the European corn borer Ostrinia nubilalis. This line had been previously engineered to accumulate carotenoids specifically in the seed endosperm, whereas the Bt gene was expressed constitutively. The concentrations of Bt toxin (Cry 1Ac) in the leaves of the 4Bt and 4BtxHC lines were not significantly different at 47±6 μg/g of fresh weight (FW); neither were they in the kernels of both lines (35±3 μg/g FW). The kernels and leaves were toxic to the larvae of O. nubilalis. However, the insecticidal activity was substantially lower (ca. 20%) than that of lines that expressed only Bt in spite that the two lines showed a quantity of toxin not significantly different in kernels or in leaves. Although the reduced effectiveness of Cry1Ac in kernels may not be entirely surprising, the observation of the same phenomenon in vegetative tissues was unexpected. When semi-artificial diets containing kernels from 4Bt supplemented with different levels of β-carotene were used in insect bioassays, the β-carotene moderated the effectiveness of the Bt similarly to the plant material with carotenoid enrichment. To elucidate the biochemical basis of the reduced effectiveness of Bt toxin in the carotenoid-enriched plants, we measured the activity of three enzymes known to be implicated in the detoxification defence, namely, catalase, superoxide dismutase and glutathione S-transferase. Whereas Cry1Ac expression significantly increased SOD and CAT enzymatic activity in the absence of carotenoids, carotenoids, either in 4BtxHC or in artificial diets enriched with β-carotene, significantly lowered CAT activity. Carotenoids can therefore moderate the susceptibility of the maize borer O. nubilalis to Cry1Ac, and we hypothesize that their role as antioxidants could explain this phenomenon via their scavenging of reactive oxygen species produced during Cry1Ac detoxification in the larvae. The involvement of this mechanism in the decreased mortality caused by Cry1Ac when carotenoids are present in the diet is discussed.

show abstract

Section: Discussionmentioning

confidence: 99%

Carotenoids moderate the effectiveness of a Bt gene against the European corn borer, Ostrinia nubilalis

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Tobacco cutworm, Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae), is a polyphagic pest, widespread throughout South and East Asia, Africa and Oceania [ 15 ]. It feeds on over 389 species of plants [ 16 ], including legumes, cotton, cabbage, cauliflower, castor, peanut, tubers and oilseeds [ 17 ]. In the states of Rajasthan and Maharashtra, India, in 2008–2009 it caused soybean losses of 64 million USD and 300 million USED respectively [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Transformations of Monoterpenes with the p-Menthane Skeleton in the Enzymatic System of Bacteria, Fungi and Insects

et al. 2020

View full text Add to dashboard Cite

The main objective of this article was to present the possibilities of using the enzymatic system of microorganisms and insects to transform small molecules, such as monoterpenes. The most important advantage of this type of reaction is the possibility of obtaining derivatives that are not possible to obtain with standard methods of organic synthesis or are very expensive to obtain. The interest of industrial centers focuses mainly on obtaining particles of high optical purity, which have the desired biological properties. The cost of obtaining such a compound and the elimination of toxic or undesirable chemical waste is important. Enzymatic reactions based on enzymes alone or whole microorganisms enable obtaining products with a specific structure and purity in accordance with the rules of Green Chemistry.

show abstract

“…Spodoptera litura (Fabricius) commonly known as tobacco cutworm is a serious polyphagous pest and is distributed throughout tropical as well temperate regions of world (Tenywa et al, 2018) It is a serious crop pest of important cash crops, vegetables, fodder, ornamental and horticulture (Ahmad et al, 2013).The larvae feeds on leaves, fresh emerging seedlings, brackets and soft shoots (Yinghua et al, 2017) and causes severe damage with yield loss ranging from 26-100% (Tuan et al, 2014). Farming community largely depends on conventional synthetic insecticides for the management of this pest.…”

Section: Introductionmentioning

confidence: 99%

Pathogenicity of Nucleopolyhedrovirus (NPV) against <i>Spodoptera litura</i> (Fabricius)

Kaur

Joshi

Sharma

et al. 2021

JBC

View full text Add to dashboard Cite

The management of Spodoptera litura by entomopathogenic Nucleopolyhedrosis Viruses (NPVs) is one of the eco-friendly methods. The present study was aimed to evaluate Nucleopolyhedrosis Virus (NPV) against S. litura and its potential as a bio-pesticide. The Occlusion Bodies (OBs) of S. litura nucleopolyhedrosis virus was isolated from infected larvae collected from cabbage field. Pathogenicity studies evaluated three NPV suspensions viz. SpltNPV-native, SpltNPV-commercial and SpltNPV-NIPHM against second and fourth instar larvae of S. litura recorded maximum mortality at 1×109 OBs/ml. The LC50 values of the SpltNPV-native, SpltNPV-commercial and SpltNPV-NIPHM suspensions against second instar larvae were 0.584, 0.540, 0.625 OBs/mm2, respectively, which increased to 0.696, 0.620, 0.756 OBs/mm2 against the fourth instar larvae. The LT50 at 1×109 OBs/ml was found to increase from 146.33, 137.51 and 155.88 h for SpltNPV-native, SpltNPV-commercial and SpltNPV-NIPHM suspension, respectively, against the second instar larvae to 178.51, 162.07 and 187.67 h, respectively, against the fourth instar larvae. The cumulative per cent mortality, LC50 and LT50 suggested that the second instar larvae were more susceptible and easier to kill than the fourth instar larvae.

show abstract

Responses of the cutworm Spodoptera litura (Lepidoptera: Noctuidae) to two Bt corn hybrids expressing Cry1Ab

Cited by 26 publications

References 52 publications

Carotenoids moderate the effectiveness of a Bt gene against the European corn borer, Ostrinia nubilalis

Carotenoids moderate the effectiveness of a Bt gene against the European corn borer, Ostrinia nubilalis

Transformations of Monoterpenes with the p-Menthane Skeleton in the Enzymatic System of Bacteria, Fungi and Insects

Pathogenicity of Nucleopolyhedrovirus (NPV) against <i>Spodoptera litura</i> (Fabricius)

Contact Info

Product

Resources

About