Responses of the cells of the supra-optic nucleus; Kinetic aspects

A quantitative in situ hybridization analysis was used to investigate changes in levels of ribosomal RNA (rRNA) in neurons of the supraoptic nucleus (SON) of rats stimulated osmotically by giving 2% NaCl as drinking solution for 0 (control rats), 1, 4, and 14 days. The quantitation was autoradiographically accomplished by in situ hybridization with a nick-translated tritiated ribosomal DNA probe and with the use of computer-based image analysis system. The mean number of grains per neuron in the ventral SON was significantly increased: 1.8-fold for 1 day, 2.9-fold for 4 days, and 1.7-fold for 14 days of salt loading, whereas the mean number of grains per neuron in the dorsal SON was increased 1.3-fold for 1 day, 2.5-fold for 4 days, and 1.7-fold for 14 days. Kolmogorov-Smirnov analysis of frequency histograms of grains per neuron indicated that the amount of rRNA in neurons in the ventral and dorsal SON was significantly increased by osmotic stimulation. These increases were accompanied by increases in cell size. The subcellular location of hybridizable rRNA in magnocellular neurons was altered by osmotic stimulation. Following 1-14 days of salt-drinking, rRNAs appeared to be more unevenly distributed throughout the cytoplasm. These findings are consistent with the notion that hyperosmotic stimulation has a substantial effect on the expression of rRNA genes in neurons of both the ventral and dorsal SON.

show abstract

Ultrastructural evidence of sexual dimorphism in supraoptic neurons: a morphometric study

Paula‐Barbosa¹,

Sousa²,

Madeira³

1993

J Neurocytol

View full text Add to dashboard Cite

We have recently shown that in spite of the absence of receptors for gonadal steroids in the supraoptic nucleus (SON) of the rat hypothalamus, the volume of the nucleus and the size of its neurons are larger in males than in females, and that these differences between male and female rats are correlated with body weight and dependent on the vasopressinergic neurons. As supraoptic neurons and their organelles enlarge when they are engaged in active peptidergic secretion we have carried out a morphometric ultrastructural analysis to determine if cell structures involved in the synthesis and storage of neurosecretory material also display weight-dependent sex dimorphism. Groups of six male and six female rats aged 30, 60 and 180 days were used. Nucleoli, rough endoplasmic reticulum and neurosecretory granules were analysed and we estimated their volume or surface densities and the total volume of nucleoli and rough endoplasmic reticulum, and total surface area of rough endoplasmic reticulum. We found that, with the exception of neurosecretory granules, the densities of the organelles did not differ among the groups studied, but total values were higher in males. These differences were found to be weight-dependent. Since the organelles studied are regarded as reliable indicators of the neurosecretory activity of supraoptic neurons, our data fully support the view that the weight-dependent sexual dimorphism observed in this nucleus reflects greater synthetic activity of its vasopressinergic neurons associated with the need to maintain water balance in larger bodies.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Responses of the cells of the supra-optic nucleus; Kinetic aspects

Cited by 9 publications

References 14 publications

Striatopallidal and Thalamic Dystonia

Striatopallidal and Thalamic Dystonia

In situ hybridization analysis of osmotic stimulus‐induced changes in ribosomal RNA in rat supraoptic nucleus

Ultrastructural evidence of sexual dimorphism in supraoptic neurons: a morphometric study

Contact Info

Product

Resources

About