Responses of Stream Biofilm Phospholipid Fatty Acid Profiles to Acid Mine Drainage Impairment and Remediation

Drerup, Samuel A.; Vis, Morgan L.

doi:10.1007/s11270-016-2856-5

Cited by 7 publications

(4 citation statements)

References 42 publications

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“…Biofilm fatty acid profiles in control and Th‐exposed conditions were not separated by the PCA and thus seemed unaffected by the exposure. It has been shown that metal contamination in acid mine drainage waters leads to fatty acid profile changes in biofilm (Drerup and Vis 2016), but the present study's Th exposure may have been too low to induce such changes. However, the centroids of the PCA points for control and Th‐exposed snails appeared to be spatially discriminated along Dim1.…”

Section: Resultscontrasting

confidence: 58%

Thorium Exposure Drives Fatty Acid and Metal Transfer from Biofilms to the Grazer Lymnaea sp.

Doose

Fadhlaoui

Morin

et al. 2021

Enviro Toxic and Chemistry

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Aquatic ecotoxicological risks associated with tetravalent metallic elements such as thorium (Th) are still poorly understood. Periphytic biofilm represents an important food source in aquatic environments; thus, such risks could severely affect nutrient and energy cycling in these ecosystems. The present study investigated the potential for Th to change the fatty acid composition of biofilm communities. Bioaccumulation of Th and fatty acids were measured after 4 wk to 2 exposure conditions: a control (C0) and Th exposure (C10). Some major fatty acids such as C16:1n-7 and docosahexaenoic acid C22:6n-3 differed significantly between control and C10 conditions. To determine if Th can be trophically transferred and to investigate the impacts of nutritional quality changes on primary consumers, common pond snails (Lymnaea sp.) were fed for 4 wk with control and Th-exposed biofilm. Thorium appeared to be trophically transferable to the grazers, although we cannot exclude that part of the Th accumulated by the snails may have been taken from the water through release from the biofilms. The composition of major fatty acids observed in the grazers was also significantly affected, notably by a decrease of total polyunsaturated fatty acids. These results indicate that very low Th concentrations can decrease the nutritional quality of organisms at the base of the food chain.

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Section: Resultscontrasting

confidence: 58%

Thorium Exposure Drives Fatty Acid and Metal Transfer from Biofilms to the Grazer Lymnaea sp.

Doose

Fadhlaoui

Morin

et al. 2021

Enviro Toxic and Chemistry

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“…This increase in algal diversity along an AMD gradient has been recorded in previous studies using morphology, molecular markers, and functional measures (Verb and Vis , Bray et al. , Drerup and Vis ).…”

Section: Discussionmentioning

confidence: 99%

“…Research investigating stream biofilm essential fatty acids reported a reduction in the proportion of PUFAs with increased AMD impairment compared to Unimpaired streams (DeForest et al. , Drerup and Vis ). Therefore, the DNA data from the current study, morphological assessments, and PUFA analyses all indicate a similar trend of decreasing diatoms with increasing AMD impairment.…”

Section: Discussionmentioning

confidence: 99%

Stream Algal Biofilm Community Diversity Along An Acid Mine Drainage Recovery Gradient Using Multimarker Metabarcoding

Wolf

Vis

2019

Journal of Phycology

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In southeastern Ohio, active remediation of streams affected by Acid Mine Drainage (AMD) has proven to be successful for some streams, while others have not recovered based on macroinvertebrate assessment. In this study, biofilms were collected from three Moderately Impaired, three Recovered, and two Unimpaired streams. The biodiversity was characterized by metabarcoding using two universal barcode markers (16S and 18S) along with two algal specific markers (UPA and rbcL) and high-throughput amplicon sequencing. For each marker, the ordination of Bray-Curtis Index calculated from the total Amplicon Sequence Variants (ASVs) present in each stream showed the Unimpaired and Recovered streams clustered, while Moderately Impaired streams were more distant. Focusing on the algal ASVs, the Shannon index for the rbcL, and UPA markers showed significantly lower alpha diversity in Moderately Impaired streams compared to Unimpaired streams, but the Recovered streams were not significantly different from the other two stream categories. The two universal markers together captured all algal phyla providing an outline of the diversity, but the two algal specific markers produced a greater number of ASVs and taxonomic depth for algal taxa. Further examination of the UPA marker revealed a drastic decrease in relative abundance of diatoms in Moderately Impaired streams compared to Recovered and Unimpaired streams. Likewise, diatom genera identified in the rbcL data and indicative of stream water quality showed marked differences in relative abundance among stream categories. Although all markers were useful, the algal-specific UPA and rbcL contributed more insights into algal community differences among stream categories.

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“…CSIA of PLFA can also serve as a valuable method for defining substrate use by microbes, providing important insights into biogeochemical processes and environmental change (e.g. [64,65]). However, interpreting PLFA results is usually only possible for broad taxonomic groups, such as gram-positive or gram-negative bacteria, fungi and phytoplankton at the class level [61,[66][67][68].…”

Section: Insights From Previous Studies: What We Have Learned (A) Natural Abundance Studiesmentioning

confidence: 99%

Stable isotopes of fatty acids: current and future perspectives for advancing trophic ecology

Twining

Taipale

Rueß

et al. 2020

Phil. Trans. R. Soc. B

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To understand consumer dietary requirements and resource use across ecosystems, researchers have employed a variety of methods, including bulk stable isotope and fatty acid composition analyses. Compound-specific stable isotope analysis (CSIA) of fatty acids combines both of these tools into an even more powerful method with the capacity to broaden our understanding of food web ecology and nutritional dynamics. Here, we provide an overview of the potential that CSIA studies hold and their constraints. We first review the use of fatty acid CSIA in ecology at the natural abundance level as well as enriched physiological tracers, and highlight the unique insights that CSIA of fatty acids can provide. Next, we evaluate methodological best practices when generating and interpreting CSIA data. We then introduce three cutting-edge methods: hydrogen CSIA of fatty acids, and fatty acid isotopomer and isotopologue analyses, which are not yet widely used in ecological studies, but hold the potential to address some of the limitations of current techniques. Finally, we address future priorities in the field of CSIA including: generating more data across a wider range of taxa; lowering costs and increasing laboratory availability; working across disciplinary and methodological boundaries; and combining approaches to answer macroevolutionary questions. This article is part of the theme issue ‘The next horizons for lipids as ‘trophic biomarkers’: evidence and significance of consumer modification of dietary fatty acids’.

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Responses of Stream Biofilm Phospholipid Fatty Acid Profiles to Acid Mine Drainage Impairment and Remediation

Cited by 7 publications

References 42 publications

Thorium Exposure Drives Fatty Acid and Metal Transfer from Biofilms to the Grazer Lymnaea sp.

Thorium Exposure Drives Fatty Acid and Metal Transfer from Biofilms to the Grazer Lymnaea sp.

Stream Algal Biofilm Community Diversity Along An Acid Mine Drainage Recovery Gradient Using Multimarker Metabarcoding

Stable isotopes of fatty acids: current and future perspectives for advancing trophic ecology

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