Responses of photosynthetic light- use efficiency and chloroplast development on exposure of leaves to ozone

Biochemical and molecular basis for impairment of photosynthetic potential

“…While long-term exposure of plants to 0 3 eventually led to a loss in chlorophyll, this appeared to be a very late event and did not provide a satisfactory explanation for initial reduction in photosynthesis (Farage et al 1991). Furthermore, in this study and several others, where the fluorescence ratio Fv/F m was measured in O3-treated foliage, either no impact was observed or the ratio was less sensitive than CO 2 fixation (see discussion below) (Farage et al 1991, Godde and Buchhold 1992, Grandjean et al 1992, Baker et al 1994. Lack of responsiveness of Photosystem II to O 3 was further illustrated by Farage et al (1991), as 0 3 did not alter the ability of thylakoids of wheat chloroplasts to bind atrazine even though other responses were detected.…”

“…Several groups have reported that the light-saturated rate of CO 2 uptake decreases significantly when plants are exposed to elevated 0 3 levels (Atkinson et al 1988, Farage et al 1991, Baker et al 1994. Farage et al (1991) explored the possibility that this decrease could be the result of reductions in pigment concentration and resultant absorption of light energy, or O3-induced reduction in the efficiency of energy transduction of the photosynthetic membrane; such effects could lead to a loss of active Photosystem II.…”

“…Since then, it has been reported that longer term relatively low doses of 0 3 resulted in reductions in the concentration of Rubisco in potato, radish, aspen, hybrid poplar, and wheat (Dann and Pell 1989, P e l l e t al. 1992, 1994b, Baker et al 1994.…”

Production Practices and Quality Assessment of Food Crops Volume 1

“…Reduction in chlorophyll content (Köllner and Krauss, 2000) and carboxylation efficiency (Reid and Fiscus, 1988;Farage et al, 1991) contribute to the observed decrease in net photosynthesis under elevated O 3 . The loss of Rubisco activity induced by elevated O 3 apparently results from a decrease in the quantity of Rubisco present (Pell et al, 1992;Baker et al, 1994). Moreover, exposure to O 3 typically reduces effective leaf area, thus decreasing light interception and the quantity of assimilate available to support the growth of economic yield component.…”

Two‐dimensional electrophoresis of acidic proteins isolated from ozone‐stressed Norway spruce needles (Picea abies L. Karst): Separation method and image processing

“…In a study of effects of exposure of mature wheat leaves to ozone, Barker etal. [21] used a similar 2-DE system. They found that a number of proteins appeared or changed their concentration after ozone fumigation.…”