Responses of Phospholipase D and Antioxidant System to Mechanical Wounding in Postharvest Banana Fruits

Abstract

Banana fruits are susceptible to mechanical damage. The present study was to investigate the responses of phospholipase D (PLD) and antioxidant system to mechanical wounding in postharvest banana fruits. During 16 d storage at 25 ∘ C and 90% relative humidity, PLD activity in wounded fruits was significantly higher than that in control (without artificial wounding fruits). The higher value of PLD mRNA was found in wounded fruits than in control. PLD mRNA expression reached the highest peak on day 4 in both groups, but it was 2.67 times in wounded fruits compared to control at that time, indicating that PLD gene expression was activated in response to wounding stress. In response to wounding stress, the higher lipoxygenase (LOX) activity was observed and malondialdehyde (MDA) production was accelerated. The activities of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and ascorbate peroxidase (APX) in wounded fruits were significantly higher than those in control. The concentrations of reactive oxygen species (ROS) such as superoxide anion (O 2•− ) and hydrogen peroxide (H 2 O 2 ) in fruits increased under mechanical wounding. The above results provided a basis for further investigating the mechanism of postharvest banana fruits adapting to environmental stress.

Effect of Nano Silicon Dioxide Coating Films on the Quality Characteristics of Fresh-Cut Cantaloupe

“…MDA is often used as a sign of fruit damage progress due to the structural integrity and cell membrane lipid peroxide levels [ 33 ]. As shown in ( Figure 3 a), a constant increase was detected for the control samples, while the treatment of nano-material delayed MDA increases.…”

Effects of Phospholipase D Inhibitors Treatment on Membrane Lipid Metabolism of Postharvest Banana Fruit in Response to Mechanical Wounding Stress

“…Banana fruits of similar size and maturity period, without infection or physical injury, were selected. Each banana was punched with four holes starting from the middle of the equator and punctured at 3-cm intervals using a stainless-steel puncher [24]. Each wound was 2.0-mm deep and 4.0-mm in diameter.…”

“…In our previous study, we provided evidence that PLD is associated with banana fruit senescence and involved in the signaling pathways in response to mechanical wounding stress [24]. We also cloned the full-length cDNA of 'Guijiao No.…”

RESPUESTAS TEMPRANAS EN SIMPLASTO DE TALLO ASOCIADAS A LA RUTA DEL ÁCIDO SALICÍLICO EN LA INTERACCIÓN CLAVEL (Dianthus caryophyllus, caryophyllaceae)- FOD (Fusarium oxysporum f. sp. dianthi)

“…La respuesta simplástica temprana en la variedad resistente descrita en el presente estudio, esta corroborada con la inducción diferencial de la enzima PLD (1 dpi), la cual se encuentra exclusivamente a nivel citosólico y está involucrada con la generación del ácido fosfatídico (PA, Phosphatidic acid), cuya molécula señal es central en la activación de respuestas a estrés biótico o abiótico (Kalachova et al, 2012;Singh et al, 2012;Li et al, 2017). Se ha demostrado que la PLD y la formación de PA son señales requeridas para la generación de especies reactivas de oxígeno (EROS) (Yamaguchi et al, 2004;Singh et al, 2012) esto debido a que puede interactuar directamente con homólogos de oxidasas (RBOH) en la membrana plasmática y regular la producción de H2O2 (Zhao, 2015;Li Wang, 2018).…”