Responses of NADH and NADPH nitrate reductase activities and nitrate uptake to chlorate supply in maize seedlings

Pandey, Sarita; Garg, Sanjay; Srivastava, H. S.

doi:10.1016/s0176-1617(97)80089-2

Cited by 3 publications

(2 citation statements)

References 22 publications

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“…Concerning nitrate assimilation, initial reductive reactions are key points of this pathway and are mediated by nitrate and nitrite reductases (Nacry et al, 2013). Two isoforms of this enzyme are ubiquitously expressed in maize roots (Pandey et al, 1997): one is NADH-dependent (E.C. 1.6.6.1) and the other NAD(P)Hdependent (E.C.…”

Section: Ofmentioning

confidence: 99%

Biostimulant Action of Dissolved Humic Substances From a Conventionally and an Organically Managed Soil on Nitrate Acquisition in Maize Plants

et al. 2020

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Conversion of conventional farming (CF) to organic farming (OF) is claimed to allow a sustainable management of soil resources, but information on changes induced on dissolved organic matter (DOM) are scarce. Among DOM components, dissolved humic substances (DHS) were shown to possess stimulatory effects on plant growth. DHS were isolated from CF and OF soil leacheates collected from soil monolith columns: first in November (bare soils) and then in April and June (bare and planted soils). DHS caused an enhancement of nitrate uptake rates in maize roots and modulated several genes involved in nitrogen acquisition. The DHS from OF soil exerted a stronger biostimulant action on the nitrate uptake system, but the first assimilatory step of nitrate was mainly activated by DHS derived from CF soil. To validate the physiological response of plants to DHS exposure, real-time RT-PCR analyses were performed on those genes most involved in nitrate acquisition, such as ZmNRT2.1, ZmNRT2.2, ZmMHA2 (coding for two high-affinity nitrate transporters and a PM H +-proton pump), ZmNADH:NR, ZmNADPH:NR, and ZmNiR (coding for nitrate reductases and nitrite reductase). All tested DHS fractions induced the upregulation of nitrate reductase (NR), and in particular the OF2 DHS stimulated the expression of both tested transcripts encoding for two NR isoforms. Characteristics of DHS varied during the experiment in both OF and CF soils: a decrease of high molecular weight fractions in the OF soil, a general increase in the carboxylic groups content, as well as diverse structural modifications in OF vs. CF soils were observed. These changes were accelerated in planted soils. Similarity of chemical properties of DHS with the more easily obtainable water-soluble humic substance extracted from peat (WEHS) and the correspondence of their biostimulant actions confirm the validity of studies which employ WEHS as an easily available source of DHS to investigate biostimulant actions on agricultural crops.

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Section: Ofmentioning

confidence: 99%

Biostimulant Action of Dissolved Humic Substances From a Conventionally and an Organically Managed Soil on Nitrate Acquisition in Maize Plants

et al. 2020

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“…Apesar da NR mais comumente encontrada ser NADH específica, já foi relatada a presença da isoforma NAD(P)H biespecífica, ou seja, capaz de utilizar tanto NADH como NADPH, com preferência para o NADPH. Em folhas, essa isoforma já foi relatada para soja (jovem) e cevada mutante para o gene que expressa a isoforma NADH específica, porém, em geral, ela parece ser expressa preferencialmente nas raízes (Jolly et al, 1976;Sueyoshi et al, 1995;Pandey et al, 1997).…”

Section: Ao Analisar Os Metabólitos Acumulados Em Baixas Temperaturasunclassified

Controle da atividade da nitrato redutase em plantas de abacaxizeiro submetidas a baixas temperaturas em diferentes fases do ciclo diurno

Matsumura¹

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EpígrafeScience is cool and fun because you get to do stuff that no one has ever done before (Children from Blackawton)Biology Letters (2011) 7, 168-172 Agradecimentos Agradeço a todos que contribuíram direta ou indiretamente na realização deste trabalho.À Profa Dra Helenice Mercier pela oportunidade e orientação, pelo incentivo e apoio em todos os momentos de meu mestrado e por ser um exemplo de dedicação.Ao Prof. Dr. Luciano Freschi pela ajuda em diversos momentos e por sempre estar disposto a ouvir e aconselhar.Ao Prof. Dr. Gilberto Kerbauy pelo apoio e por ser um exemplo de dedicação e entusiasmo pela pesquisa.À doutoranda Cássia pela ajuda com os experimentos e pela orientação em diversos momentos.À mestre Alessandra pelo auxílio com as técnicas da NR in vitro e fracionamento e pela disposição em ajudar.A todos do laboratório (alunos e ex-alunos) pela amizade, apoio e pelo ambiente sempre agradável: Alejandra, Paulo, Paula, Perdigão, Auri, Lucas, Rafael, Nielda, Bruno, Dioceni, Ilton, Lia, Michael, Bruna. Ao Leonardo e Oscar pela ajuda com os experimentos.Aos técnicos e ex-técnicos do laboratório pelo trabalho, amizade e disposição em ajudar:

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Iron Mediated Nitrate Reductase Activity in Different Parts of Young Maize Seedlings

Pandey¹

2000

Biol. plant.

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Responses of NADH and NADPH nitrate reductase activities and nitrate uptake to chlorate supply in maize seedlings

Cited by 3 publications

References 22 publications

Biostimulant Action of Dissolved Humic Substances From a Conventionally and an Organically Managed Soil on Nitrate Acquisition in Maize Plants

Biostimulant Action of Dissolved Humic Substances From a Conventionally and an Organically Managed Soil on Nitrate Acquisition in Maize Plants

Controle da atividade da nitrato redutase em plantas de abacaxizeiro submetidas a baixas temperaturas em diferentes fases do ciclo diurno

Iron Mediated Nitrate Reductase Activity in Different Parts of Young Maize Seedlings

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