Responses of micropropagated peach cultivars to thermotherapy for the elimination of prunus necrotic ringspot virus

Stein, A.; Spiegel, S.; Faingersh, G.; Lévy, Samuel

doi:10.1111/j.1744-7348.1991.tb04865.x

Cited by 22 publications

(18 citation statements)

References 11 publications

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“…Most often, the temperature of thermotherapy treatments is measured in a growth chamber. Some authors have shown that the air temperature measured in the growth chamber was 1 or 2°C (Stein et al 1991;Deogratias et al 1989) lower than that inside the test tubes. When choosing a temperature, there must be a balance between relatively low shoot mortality at higher temperatures and the high percentage of virus-free shoots (Dziedzic 2008).…”

Section: Discussionmentioning

confidence: 94%

Virus elimination from in vitro apple by thermotherapy combined with chemotherapy

Dong

Zhang

et al. 2015

Plant Cell Tiss Organ Cult

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We evaluated the effectiveness of thermotherapy at different temperatures, chemotherapy with different concentrations of ribavirin, and combinations of these two methods on virus elimination from apple. We used Malus cv. 'Xinhongjiangjun', an apple cultivar widely grown in China, infected with Apple chlorotic leaf spot virus (ACLSV), Apple stem grooving virus (ASGV), and Apple stem pitting virus (ASPV). The survival and regeneration of plants were evaluated, and the efficiency of virus eradication was determined by reverse-transcription polymerase chain reaction with two primer pairs for each virus. All of the plants treated with 15 and 25 lg/ml ribavirin survived, although their proliferation was slightly inhibited. Ribavirin treatments at 15 and 25 lg/ml resulted in virus elimination rates of 74.4 and 75.0 %, respectively. Higher temperatures significantly affected the growth and proliferation of plants, and almost no axillary shoots regenerated under the highest temperature (38 ± 0.5°C). The average virus elimination rate after 34-36°C treatments for 20 days was no more than 45 %. The virus elimination efficiency was enhanced by combining thermotherapy and chemotherapy treatments. A treatment combining ribavirin (25 lg/ml) and thermotherapy at 36°C (R25 ? T36) resulted in high virus eradication efficiency (95.0 %). Across all of the treatments, the average survival rate of apical shoots (176/273) was 12 % lower than that of axillary shoots (266/349); the average virus elimination efficiency was 65.3 % for apical shoots and 72.7 % for axillary shoots. The results also demonstrated that it was easier to eliminate ASPV than to eliminate ACLSV and ASGV.

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Section: Discussionmentioning

confidence: 94%

Virus elimination from in vitro apple by thermotherapy combined with chemotherapy

Dong

Zhang

et al. 2015

Plant Cell Tiss Organ Cult

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“…However, when the plants were transferred after a few hours at 40°C to an optimal temperature, host RNA synthesis was resumed immediately, while virus synthesis was delayed (Dawson and Lozoya Saldana 1984). The effect on viral RNA synthesis could probably be magnified if cycles of high and low temperature are repeated, thereby allowing elimination of the virus from growing parts of the plants in relative short periods of time (Stein et al 1991).…”

Section: Thermotherapy Applied To Intact Plantmentioning

confidence: 98%

“…Water bath treatment was found to be more effective as compared to moist hot air treatment. In practice, the temperature chosen for thermotherapy is a compromise between reasonable plant survival and virus elimination (Stein et al 1991). In the present study, water bath treatment at 50°C for 120 min was found to be most effective as it resulted in ICRSV elimination in 36.84% of the successful micrografts coupled with reasonable percentage of shoot formation (41.67%) in nodal bud explants.…”

Section: Thermotherapy Applied To Nodal Segmentsmentioning

confidence: 99%

In vitro production of Indian citrus ringspot virus (ICRSV) free Kinnow plants employing thermotherapy coupled with shoot tip grafting

Sharma

Singh

Rani

et al. 2007

Plant Cell Tiss Organ Cult

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Indian citrus ringspot virus (ICRSV) is known to cause serious disease problem in Kinnow (Citrus nobilis Lour · C. deliciosa Tenora) plants. This work reports the elimination of ICRSV by using thermotherapy coupled with shoot tip grafting in vitro. Nodal segments from infected mother plants (indexed by indirect ELISA and RT-PCR) were treated both in water bath and moist hot air at different temperatures viz. 40, 45 and 50°C for 30, 60 and 120 min and cultured on MS medium containing 2-iP (1 mg/l) and malt extract (800 mg/l). Shoot tips were excised from the nodal sprouts and grafted on to rough lemon (C. jambhiri) rootstock under aseptic conditions. Water bath treatment was found to be more effective as compared to moist hot air treatment as maximum number of ICRSV free plants (36.84%) were obtained by grafting the tips (0.7 mm) taken from the nodal segments treated at 50°C in water bath for 2 h. In an alternate treatment regime, 1-year-old infected plants were kept at various temperatures viz.36, 38 and 40°C in a thermotherapy chamber. Maximum of 60% ICRSV free plants were obtained by grafting the tips (0.7 mm) from the plants placed at 40°C followed by the plants placed at 38°C (59.09%) and the least was observed in case of the plants placed at 36°C (40.74%). Only those plants/plantlets were considered virus free, which showed negative reaction both in Indirect ELISA and RT-PCR.

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“…Several protocols have been reported to eliminate viruses infecting Prunus species by tissue culture, but the protocols were tested only for some pathogens in limited species and cultivars (Navarro et al, 1982;Stein et al, 1991;Howell et al, 2001;Rizqi et al, 2001). Application of in vitro pathogen elimination technique depends on establishing plant materials in tissue culture.…”

Section: Introductionmentioning

confidence: 99%

Effect of Carbohydrates on in vitro Shoot Growth of Various Prunus Species

Cheong¹,

An²

2015

Korean Journal of Plant Resources

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-Carbohydrate sources are one of important factors associated with macro-and micro nutrients and phytohormones in vitro culture medium for shoot growth. The optimal carbohydrates for eight species of the genus Prunus which are economically important fruit crop was evaluated at the initiation and elongation stages. All carbohydrate seemed utilized for the bud break and leaf growth at the early stage of culture. However, shoot elongation and fresh weight of species tested were superior in the medium containing 90 mM of fructose or glucose rather than sucrose. There was no difference between glucose and fructose. Adventitious shoots from the axillary buds were induced in most species but no significant differences were observed except for two species (P. salicina 'Shiro' and P. tomentosa). These result demonstrated that glucose and fructose were suitable carbohydrate sources for diverse Prunus species than sucrose, although the response to the carbohydrates in the medium were slightly different in the species.

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Responses of micropropagated peach cultivars to thermotherapy for the elimination of prunus necrotic ringspot virus

Cited by 22 publications

References 11 publications

Virus elimination from in vitro apple by thermotherapy combined with chemotherapy

Virus elimination from in vitro apple by thermotherapy combined with chemotherapy

In vitro production of Indian citrus ringspot virus (ICRSV) free Kinnow plants employing thermotherapy coupled with shoot tip grafting

Effect of Carbohydrates on in vitro Shoot Growth of Various Prunus Species

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