Responses of <i>Candida fukuyamaensis</i> RCL‐3 and <i>Rhodotorula mucilaginosa</i> RCL‐11 to copper stress

Villegas, Liliana Beatriz; Amoroso, Maria Julia del R.; Figueroa, Lucía I. C. de

doi:10.1002/jobm.200800218

Cited by 24 publications

(17 citation statements)

References 33 publications

(40 reference statements)

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“…Intracellular accumulation of copper and morphological changes were observed, indicating that the resistance mechanism in R. mucilaginosa RCL-11 works through sequestration and extrusion of cytoplasmic copper ions (Villegas et al 2009). …”

Section: Resultsmentioning

confidence: 97%

“…Similarly, a diminished CuZnSOD activity is directly responsible for increased sensitivity of yeast mutant cells to oxidative stress (Irazusta et al 2010). Studies with R. mucilaginosa RCL-11 have demonstrated an increase in SOD activity when cells were exposed to increasing exposure times and copper concentrations (Villegas et al 2009). In addition, the yeast Yarrowia lipolytica was able to grow at high concentrations of copper sulfate and showed higher CuZnSOD activity under these conditions (Ito et al 2007).…”

Section: Resultsmentioning

confidence: 98%

“…When cultured with Cu (II), Rhodotorula mucilaginosa RCL-11 decreased its growth rate with increasing Cu (II) concentration, and it also absorbed this metal ion from the culture medium. It was shown that R. mucilaginosa had increased rates of superoxide dismutase (SOD) and catalase (CAT) as a consequence of oxidative stress due to elevated Cu (II) concentrations (Villegas et al 2009). However, so far no studies have been conducted to identify novel proteins associated with this resistance.…”

Section: Introductionmentioning

confidence: 98%

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Proteomic study of the yeast Rhodotorula mucilaginosa RCL-11 under copper stress

et al. 2012

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In order to understand the mechanism involved in Rhodotorula mucilaginosa RCL-11 resistance to copper a proteomic study was conducted. Atomic absorption spectroscopy showed that the copper concentration in the medium decreased from 0.5 to 0.19 mM 48 h after inoculation of the yeast. Analysis of one-dimensional gel electrophoresis of crude cell extracts revealed expression of differential bands between cells with and without copper. In order to study this difference, two-dimensional electrophoresis of R. mucilaginosa RCL-11 exposed to Cu for 16, 24, and 48 h was carried out. Identification of differentially expressed proteins was performed by MALDI-TOF/TOF. Ten of the 16 spots identified belonged to heat shock proteins. Superoxide dismutase, methionine synthase and beta-glucosidase were also found over-expressed at high copper concentrations. The results obtained in the present work show that when R. mucilaginosa RCL-11 is exposed to 0.5 mM copper, differential proteins, involved in cell resistance mechanisms, are expressed.

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Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 98%

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Proteomic study of the yeast Rhodotorula mucilaginosa RCL-11 under copper stress

et al. 2012

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“…The number of dark bodies in the cells increased with increasing incubation time. Scanning electron micrographs revealed that R. mucilaginosa RCL-11 cells grown in the presence of Cu(II) were larger than those grown in the absence of the heavy metal (Villegas et al 2009 ). An increase in catalase (CAT) and superoxide dismutase (SOD) activity correlated with Cu(II) concentrations was observed in R. mucilaginosa RCL-11 cells (Villegas et al 2009 ).…”

Section: Rhodototula Mucilaginosa Rcl-11mentioning

confidence: 98%

“…Similarly, a diminished CuZnSOD activity is directly responsible for increased sensitivity of yeast mutant cells to oxidative stress (Irazusta et al 2010 ). Studies with R. mucilaginosa RCL-11 have demonstrated an increase in superoxide dismutase activity when cells were exposed to increasing exposure times and copper concentrations (Villegas et al 2009 ). In addition, the yeast Yarrowia lipolytica was able to grow at high concentrations of copper sulfate and showed higher CuZnSOD activity under these conditions (Ito et al 2007 ).…”

Section: Superoxide Dismutasementioning

confidence: 98%

Copper Resistance and Oxidative Stress Response in Rhodotorula mucilaginosa RCL-11. Yeast Isolated from Contaminated Environments in Tucumán, Argentina

Irazusta

Figueroa

2014

Bioremediation in Latin America

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The threat of heavy metal pollution to public health and wildlife has led to a great interest in the development of effective technologies for heavy metal immobilization in a non-bioavailable form or their conversion into less toxic forms. Organisms subjected to metal exposure in their natural environments have developed resistance mechanisms such us dedicated components and sophisticated homeostasis. Rhodotorula mucilaginosa RCL-11, a pigmented yeast isolated from a fi lter plant of a copper mine in the province of Tucumán, Argentina, supports high concentrations of the heavy metal Cu(II). In order to understand the mechanism involved in resistance to copper in this yeast, a proteomic study was conducted. Identifi cation of differentially expressed proteins was performed. The results obtained show that when R. mucilaginosa RCL-11 was exposed to 0.5 mM copper, differential proteins, involved in cell resistance mechanisms, were expressed. Moreover, copper overload augmented carotenoid biosynthesis in this yeast, modifying at the same time the relative proportion of the pigments produced. Inhibition of the synthesis pathway with diphenylamine suggests an inverse relationship between carotenoid and copper biosorption by R. mucilaginosa RCL-11. The increased activity of superoxide dismutase and catalase measured under inhibition of carotenoid biosynthesis could explain these observations. The change in the relative proportion of the carotenoids torularhodin, torulene, and beta-carotene, as well as the detection of gamma-carotene in the presence of Cu(II) allows to hypothesize that the carotenoids produced by R. mucilaginosa RCL-11 play different roles in the oxidative stress response of this yeast.

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Disentangling metabolic pathways involved in copper resistance in Candida fukuyamaensis RCL‐3 indigenous yeast

Irazusta

Michel

Figueroa

2015

J. Basic Microbiol.

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Candida fukuyamaensis RCL-3 yeast strain isolated from a copper filter plant is able to lower copper concentration in culture medium. In the present study, effect of copper in proteins expression and mechanisms involved in copper resistance were explored using comparative proteomics. Mono-dimensional gel electrophoresis revealed differential band expressions between cells grown with or without copper. 2-DE analysis of C. fukuyamaensis RCL-3 revealed that copper exposure produced at least an over-expression of 40 proteins. Sixteen proteins were identified and grouped in four categories according to their functions: glycolysis and ATP production, synthesis of proteins, oxidative stress response, and processing and transport of proteins. Integral membrane proteins and membrane-associated proteins were analyzed, showing nine protein bands over-expressed in Cu-supplemented medium. Four proteins were identified, namely nucleoporin pom152, elongation factor 2, copper chaperone Sod1 Ccs1, and eiosome component Lsp1. The proteomic analysis performed allowed the identification of different metabolic pathways and certain proteins involved in metal input and storage related to cell ability to bioremediate copper. These proteins and mechanisms could be used for future applications of C. fukuyamaensis RCL-3 in biotechnological processes such as remediation of heavy metals.

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Responses of Candida fukuyamaensis RCL‐3 and Rhodotorula mucilaginosa RCL‐11 to copper stress

Cited by 24 publications

References 33 publications

Proteomic study of the yeast Rhodotorula mucilaginosa RCL-11 under copper stress

Proteomic study of the yeast Rhodotorula mucilaginosa RCL-11 under copper stress

Copper Resistance and Oxidative Stress Response in Rhodotorula mucilaginosa RCL-11. Yeast Isolated from Contaminated Environments in Tucumán, Argentina

Disentangling metabolic pathways involved in copper resistance in Candida fukuyamaensis RCL‐3 indigenous yeast

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