Responses of haptoglobin and serum amyloid A in goats inoculated intradermally with C. pseudotuberculosis and mycolic acid extract immunogen

Abstract

Haptoglobin (Hp) and Serum Amyloid A (SAA) are a group of blood proteins whose concentrations in animals can be influenced by infection, inflammation, surgical trauma or stress. Corynebacterium pseudotuberculosis is the causative agent of caseous lymphadenitis (CLA), and Mycolic acid is a virulent factor extracted from C. pseudotuberculosis. There is a dearth of sufficient evidence on the clinical implication of MAs on the responses of Hp and SAA in goats. Therefore, this study was conducted to evaluate the potential effects of Mycolic acid (MAs) and C. pseudotuberculosis on the responses of Hp and SAA in female goats. A total of 12 healthy female goats was divided into three groups; A, B and C each comprising of 4 goats and managed for a period of three months. Group (A) was inoculated with 2 mL of sterile phosphate buffered saline (as a negative control group) intradermally, while group (B) and (C) were inoculated intradermally with 2 ml each of mycolic acid and 1‏ × 10 cfu of active C. pseudotuberculosis respectively. The result of the study showed that the Hp concentration in goats inoculated with C. pseudotuberculosis was significantly increased up to 7-fold (1.17 ± 0.17 ng/L) while MAs showed a 3-fold increased (0.83 ± 0.01 ng/L) compared with the control. Whereas SAA concentration in C. pseudotuberculosis and MAs groups showed a significant 3-fold (17.85 ± 0.91 pg/mL) and 2-fold (10.97 ± 0.71 pg/mL) increased compared with the control. This study concludes that inoculation of C. pseudotuberculosis and MAs have significant effects on Hp and SAA levels, which indicates that MAs could have a role in the pathogenesis of caseous lymphadenitis.

Silencing of SAA1 inhibits palmitate- or high-fat diet induced insulin resistance through suppression of the NF-κB pathway

“…SAA, which is predominantly found in the bloodstream at 0.1 μM, is an acute-phase protein with apolipoprotein properties that has been implicated in the development of a number of pathological conditions, including type 2 diabetes mellitus (Baranova et al 2005; Lakota et al 2018). SAA, a classical, acute-phase protein and a product of hepatocytes, could potentially respond to infection, injury and inflammation and also has the ability to regulate toll-like receptor 4 (TIR4) which is associated with obesity-induced insulin resistance (Odhah et al 2018; Sandri et al 2008; Shi et al 2006; Tannock et al 2018). SAA1 has been identified in literature as a 12- to 14-kDa apolipoprotein and an acute phase reactant (Baranova et al 2005; Hinrichs et al 2018).…”

Klinik Olarak Pnömoni Tespit Edilen Keçilerde Serum Amiloid-A Düzeylerinin Değerlendirilmesi

“…(Table 2) (Jeber et al 2016). The authors reported that this increase is due to the fact that hepatocytes and adipocytes produce SAA that is an acute phase marker that can respond swiftly to infections (Odhah et al, 2018). Gonzalez.…”

Linfadenitis caseosa: factores de virulencia, patogénesis y vacunas. Revisión

“…Otras enzimas que participan en la síntesis son la AccD1 para la elongación de la cadena de ácidos micólicos y la FadD una AMP ligasa (29) . La inoculación de ácidos corynomicólicos en ovinos demostró que la haptoglobina (Hp) aumentó tres veces su concentración, así como también dos veces los niveles de amiloide sérico A (SAA), proteínas indicadoras de inflamación e infecciones agudas (42) . Estos resultados indican su potencial virulento, ya que por sí solos, son capaces de inducir en el hospedero reacciones de inflamación, que contribuye a la formación de granulomas.…”