Responses of growth and primary metabolism of water-stressed barley roots to rehydration

Sicher, Richard C.; Timlin, Dennis; Bailey, Bryan A.

doi:10.1016/j.jplph.2012.01.002

Cited by 90 publications

(55 citation statements)

References 32 publications

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“…Proline plays a key role in not only osmotic protection but also protection of membranes from lipid peroxidation. Generally, drought can cause proline accumulation that is independent of changes in other amino acids (Sicher et al 2012). A similar result was also found in our study (Fig.…”

Section: Changes In Carbon Metabolism During Drought and Recoverysupporting

confidence: 91%

Photosynthetic carbon and nitrogen metabolism and the relationship between their metabolites and lipid peroxidation in dwarf bamboo (Fargesia rufa Yi) during drought and subsequent recovery

Liu

Wang

Pan

et al. 2015

Trees

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Key message Differential regulations of C and N metabolism in dwarf bamboo improve the capacity of osmotic adjustment, and also their metabolites may play an important role for protection against membrane lipid peroxidation under drought, thus accelerating recovery after rewatering. Abstract Dwarf bamboo (Fargesia rufa Yi), is a staple food for the endangered giant panda, but little is known about the impact of drought on bamboo species and its recovery mechanism. This study investigated the response of carbon (C) and nitrogen (N) metabolism to drought and subsequent recovery, and the relationship of their metabolites with lipid peroxidation. Photochemistry was reversibly down-regulated after drought, but a longer recovery time is needed. The accelerated degradation of starch due to a rapid increase in amylase activity resulted in higher soluble sugar only in severe drought-stressed plants after 30 days of drought. Sucrose content was not affected by drought because of the relative increases in activities of invertase, sucrose synthase, and sucrose phosphate synthase. As nitrate concentration increased in parallel with nitrate reductase activity, ammonium (NH 4 ? ) production was enhanced by drought. Also, activated glutamine synthetase/glutamate synthase cycle stimulated NH 4? assimilation, while hydrolysis of soluble proteins was accelerated, resulting in accumulation of amino acids. After rewatering, re-balancing of C metabolism has gradually begun, but a stronger N metabolism was still observed. The notable positive correlations between MDA and the contents of starch and proline after 15 days of drought as well as between MDA and the contents of soluble sugar, NSC and proline after 30 days of drought were displayed. We conclude that dwarf bamboo may not only differently regulate its C and N metabolism to improve the capacity of osmotic adjustment but also employ its different metabolites protect against membrane Communicated by M. Adams.

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Section: Changes In Carbon Metabolism During Drought and Recoverysupporting

confidence: 91%

Photosynthetic carbon and nitrogen metabolism and the relationship between their metabolites and lipid peroxidation in dwarf bamboo (Fargesia rufa Yi) during drought and subsequent recovery

Liu

Wang

Pan

et al. 2015

Trees

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“…Their level might be affected by different stresses, as the carbohydrate content is related to photosynthesis. Contents of some carbohydrates including sucrose, raffinose, glucose, fructose and maltose increased, whereas the myoinositol level decreased in water-stressed barley roots (Sicher et al 2012). Soluble sugars function as osmoprotectants during water deficit, reducing the detrimental effects of osmotic stress, helping in maintaining turgor, stabilizing cell membranes and protecting plants from degradation (Basu et al 2007).…”

Section: Carbohydratesmentioning

confidence: 98%

Influence of abiotic stresses on plant proteome and metabolome changes

et al. 2013

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Plant responses to abiotic stresses are very complex phenomena with individual characteristics for various species. Abiotic stresses (e.g. drought, salinity, flooding, cold, heat, UV radiation, heavy metals, etc.) strongly affect plant growth and development. It is estimated that they are the cause of more than 50 % of crop yield losses. Abiotic stresses are known to activate a multigene response resulting in the changes in various proteins and primary and secondary metabolite accumulation. Therefore, proteomic and metabolomic approaches are becoming very important and powerful tools used in studying plants' reaction to various stimuli. Precise analysis of proteome and metabolome is essential for understanding the fundamentals of stress physiology and biochemistry. In this review, we focus on recent reports concerned to the influence of abiotic stresses on changes in the level of different protein groups and metabolite classes. Basic information about physicochemical methods applied to qualitative and quantitative analyses of biopolymers and natural products is also briefly presented.

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“…With the objective of improving the ability of barley plants to adapt to phosphate absence in the environment, Huang et al (2008) analyzed changes of metabolic profiles in carbon and nitrogen metabolism in the shoots and roots of barley plants grown on phosphate-deficient soil. Recently, changes in polar primary metabolites of water-stressed barley roots and the responses to rehydration have been investigated (Sicher et al 2012). However, metabolite profiling-based investigations of grain from barley subjected to drought stress have not been reported yet.…”

Section: Introductionmentioning

confidence: 99%

Impact of induced drought stress on the metabolite profiles of barley grain

et al. 2014

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The aim of the study was to investigate the impact of drought stress on the metabolite profiles of barley (Hordeum vulgare L.) grain against the background of natural variability depending on growing location and season. Six barley genotypes were field-grown (i) under normal weather conditions at two different sites and (ii) under induced drought conditions, using a Rain-Out-Shelter. Both trials were performed in three consecutive seasons (2010)(2011)(2012). Samples were subjected to a gas chromatography-mass spectrometry metabolite profiling procedure, based on the extraction and fractionation of a broad spectrum of low molecular weight metabolites ranging from lipophilic (e.g. triglyceride-derived fatty acids, free fatty acids, fatty alcohols, sterols) to hydrophilic (e.g. sugars, sugar alcohols, acids, amino acids and amines) compounds. The comparative assessment of the profiling data by means of multivariate analyses revealed that differences in lipophilic metabolites were mainly due to seasonal impact. In contrast water deficit was strongly reflected in quantitative changes of polar metabolites, irrespective of natural variability. The impact factor growing location was differently pronounced depending on the growing season. Univariate statistical analysis revealed 17 metabolites, including the monosaccharides fructose and glucose, the trisaccharide raffinose, several organic acids and the biogenic amine c-aminobutyric acid to be significantly (p-value \ 0.01) influenced by drought stress conditions.

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Responses of growth and primary metabolism of water-stressed barley roots to rehydration

Cited by 90 publications

References 32 publications

Photosynthetic carbon and nitrogen metabolism and the relationship between their metabolites and lipid peroxidation in dwarf bamboo (Fargesia rufa Yi) during drought and subsequent recovery

Photosynthetic carbon and nitrogen metabolism and the relationship between their metabolites and lipid peroxidation in dwarf bamboo (Fargesia rufa Yi) during drought and subsequent recovery

Influence of abiotic stresses on plant proteome and metabolome changes

Impact of induced drought stress on the metabolite profiles of barley grain

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