Responses of Epithelial-like Cells in Tissue Culture to Implant Materials

Sisca, R.F.; Thonard, John C.; Lower, D.A.; George, W. Arthur

doi:10.1177/00220345670460011901

Cited by 30 publications

(5 citation statements)

References 6 publications

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“…For decades, investigators have studied the toxicity and biocompatibility of implant materials using in vivo systems (3)(4)(5)28). Alternately, several groups have shown that in vitro systems are pertinent for examining the interactions of cells with implant materials (2,13,22). In fact, Johnson et al demonstrated that their in vitro assays were more sensitive to differences in material composition than a 90-day assay involving subcutaneous implantation in rats (1 1).…”

Section: Discussionmentioning

confidence: 99%

In vitro bone biocompatibility of poly(anhydride‐co‐imides) containing pyromellitylimidoalanine

Attawia

Uhrich

Botchwey

et al. 1996

Journal Orthopaedic Research

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Poly(anhydride-co-imides) are currently under study for applications involving bone. The cytotoxicity of a series of poly(anhydride-co-imides) with osteoblast-like cells (MC3T3-E1) was evaluated. The imide component of the copolymers was based on pyromellitylimidoalanine and the anhydride component was based on either sebacic acid or 1,6-bis(carboxyphenoxy)hexane. Cell adhesion and proliferation on the surfaces of the polymer discs were observed by environmental scanning electron microscopy. During the first 24 hours of attachment, the cells showed normal morphology when cultured on copolymers containing 1,6-bis(carboxyphenoxy)hexane. The cells did not adhere to the polymers containing sebacic acid, probably due to the rapid degradation of the polymer surfaces. Concurrently, the effects of polymer breakdown products on osteoblast-like cells were evaluated by studying their proliferation (cell numbers), viability (dye exclusion), morphology (light microscopy), and phenotypic expression. The morphology of osteoblast-like cells cultured in the presence of the polymer breakdown products pyromellitylimidoalanine and pyromellitic acid was found to be similar to that of the same cells grown on tissue culture polystyrene and consisted of a characteristic polygonal shape. With use of a monoclonal antibody to osteocalcin, these cells were shown to demonstrate preserved osteoblast phenotype with growth over a 21-day period. In addition, the cells reached confluency after 3-4 days, similar to cells grown on tissue culture polystyrene. This in vitro evaluation showed that the poly(anhydride-co-imides) evaluated are non-cytotoxic and may be viable biomaterials for orthopaedic applications.

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Section: Discussionmentioning

confidence: 99%

In vitro bone biocompatibility of poly(anhydride‐co‐imides) containing pyromellitylimidoalanine

Attawia

Uhrich

Botchwey

et al. 1996

Journal Orthopaedic Research

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“…Direct cell/material contact for non-water-soluble materials can be established in the following ways: The test specimen is placed as close to the tissue explant as possible [52]. …”

Section: Local Adverse Reactions and Evaluation Systemsmentioning

confidence: 99%

Biocompatibility of Resin-based Dental Materials

2009

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Oral and mucosal adverse reactions to resin-based dental materials have been reported. Numerous studies have examined the biocompatibility of restorative dental materials and their components, and a wide range of test systems for the evaluation of the biological effects of these materials have been developed. This article reviews the biological aspects of resin-based dental materials and discusses the conventional as well as the new techniques used for biocompatibility assessment of dental materials.

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“…Most previous workers have concentrated on gaining as intimate a contact as possible between the test material and the cells in culture to allow the fullest expression of its toxic effect. The materials have usually been placed directly into the culture medium either as prepared set specimens (Ochi, 1964;Rappaport et al, 1964;Kereszetesi & Kellner, 1966;Sisca et al, 1967;Kawahara et al, 1968;, 1973Leirskar, 1974a, b;, or in the freshly mixed state as a solution or suspension (Engstrom & Spangberg, 1961Q. ;Spangberg, 1969c, d, e).…”

Section: Methods Of Establishing Cell-materials Contactmentioning

confidence: 99%

“…Many early workers used HeLa cells, a human epithelial line derived from a carcinoma of the uterine cervix (Rappaport, Lilly & Kapsimalis, 1964;Engstrom & Spangberg, 1967a, b;Spangberg & Engstrom, 1967). Sisca et al (1967) used two permanent cell lines, F/L cells, derived from normal human amnion, and WISH cells (from a similar source), but did not report any difference in the results. Spangberg (1969d) compared the effect of root canal materials on HeLa cells and human embryonic fibroblasts, and found that in general the fibroblasts were slightly less sensitive.…”

Section: Cell Typementioning

confidence: 99%

“…However, the need to introduce the material into the test system freshly mixed, and for it then to set under conditions similar to those pertaining clinically, presents a major problem. The majority of in vitro studies previously reported used either prepared specimens of the set material (Ochi, 1964;Rappaport et al, 1964;Keresztesi & Kellner, 1966;Sisca et al, 1967;Kawahara, Yamagami & Nakamura, 1968;, 1973Leirskar, 1974a, b;, and thereby its most toxic state was not studied, or added the freshly mixed material into the culture medium thereby affecting its normal setting process (Engstrom & Spangberg, 1967a;Spangberg, 1969a). Although these methods have contributed to the study of some aspects of dental material cytotoxicity', they cannot substitute for in vivo methods.…”

Section: Physical State Of the Test Materialsmentioning

confidence: 99%

See 1 more Smart Citation

Biological testing of dental restorative materials in vitro—a review

Browne

Tyas

1979

J of Oral Rehabilitation

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In vitro methods for the biological evaluation of dental restorative materials are discussed, with particular reference to the type of cells used, the physical state of the test material, the method of establishing cell-material contact, and the criteria of cytotoxicity. It is postulated that in vitro methods should provide a reliable assessment of the toxicity of dental restorative materials, but since the current in vitro methods do not mimic in vivo conditions sufficiently closely, the correlation between in vitro and in vivo results is at present poor. The development of in vitro methods more closely simulating in vivo circumstances is advocated.

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Responses of Epithelial-like Cells in Tissue Culture to Implant Materials

Cited by 30 publications

References 6 publications

In vitro bone biocompatibility of poly(anhydride‐co‐imides) containing pyromellitylimidoalanine

In vitro bone biocompatibility of poly(anhydride‐co‐imides) containing pyromellitylimidoalanine

Biocompatibility of Resin-based Dental Materials

Biological testing of dental restorative materials in vitro—a review

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