Responses of Antioxidant Defense and Immune Gene Expression in Early Life Stages of Large Yellow Croaker (Pseudosciaena crocea) Under Methyl Mercury Exposure

Wu, Fangzhu; Huang, Wei; Liu, Qiang; Xu, Xiaoqun; Zeng, Jiangning; Cao, Liang; Hu, Jian; Xu, Xudan; Gao, Yuexin; Jia, Shenghua

doi:10.3389/fphys.2018.01436

Cited by 26 publications

(18 citation statements)

References 59 publications

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“…The CAT activity in the serum increased in crab fed the PCB-only diet compared with the control group, and dietary vitamin A addition effectively improved this condition. The induction of CAT activity is related to oxidative stress leading to the continuous generation of H 2 O 2 from O2−, and the activated CAT activity could protect the organism by scavenging H 2 O 2 (Wu et al, 2018). In the hepatopancreas, the GPx activity was significantly decreased by dietary PCB exposure, but dietary vitamin A supplementation could not relieve the negative effects.…”

Section: Discussionmentioning

confidence: 99%

Dietary Aroclor 1254-Induced Toxicity on Antioxidant Capacity, Immunity and Energy Metabolism in Chinese Mitten Crab Eriocheir sinensis: Amelioration by Vitamin A

Feng

Wang

et al. 2019

Front. Physiol.

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Effects of dietary Polychlorinated biphenyl (PCB) exposure and dietary vitamin A supplementation on Chinese mitten crab Eriocheir sinensis were studied with the aim to explain dietary PCB toxicity and toxic alleviation by vitamin A intake in crab. Four diets were used including three experimental diets containing 0, 80000 or 240000 IU/kg vitamin A with each experimental diet containing 10 mg PCB/kg diet, and a control diet (without vitamin A and PCB supplementation) in 56 days feeding trial. Crabs fed the PCB-only diet had significantly lower weight gain than those fed the control diet. No significant difference was observed in crab survival among all groups. Crabs fed the PCB-only diet had a significantly higher malondialdehyde content and antioxidase superoxide dismutase activity in the serum and hepatopancreas, and higher erythromycin N-demethylase and glutathione S-transferase activities in the hepatopancreas than those fed the control diet. However, supplementation of dietary vitamin A decreased the levels of all these parameters. The hepatopancreatic cytochrome P450 2 and 4 (CYP2, CYP4), fatty acid binding proteins 3 and 10 (FABP3, FABP10) and intracellular lipolytic enzyme (IL) Messenger Ribonucleic Acid (mRNA) levels in the PCB-only group were significantly higher than those in the control group, and dietary 240000 IU/kg vitamin A supplementation decreased hepatopancreatic CYP4, FABP3, FABP10 and IL enzyme mRNA level. The crabs fed 80000 IU/kg vitamin A supplementation diet had the highest level of retinoid X receptor mRNA in the hepatopancreas. The structure of the hepatopancreas was damaged and the deposit of lipid droplets decreased with dietary PCB exposure. Both levels of vitamin A supplementation alleviated the damage and increased lipid droplets in the hepatopancreas. Dietary PCB exposure significantly reduced total hemocyte count (THC), and phenoloxidase, acid phosphatase activities in the serum. Post-challenge survival of crab in the experimental PCB-only diet group was low compared with that in the control. Supplementation of 240000 IU/kg vitamin A significantly increased the THC and phenoloxidase activity in the serum and post-challenge survival compared with those in the PCB-only group. This study indicates that dietary vitamin A can improve the antioxidant capacity, immune response, detoxification enzymes activities, energy metabolism and hepatopancreas tissue structure of Chinese mitten crab fed PCB contaminated diets.

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Section: Discussionmentioning

confidence: 99%

Dietary Aroclor 1254-Induced Toxicity on Antioxidant Capacity, Immunity and Energy Metabolism in Chinese Mitten Crab Eriocheir sinensis: Amelioration by Vitamin A

Feng

Wang

et al. 2019

Front. Physiol.

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“…It is an indisputable fact that relative low dose of MeHg could cause death, genotoxicity, neurotoxicity, cytotoxicity, immunotoxicity, and endocrine toxicity to fish (Cambier et al, 2012;Wu et al, 2018;Farina and Aschner, 2019). Because MeHg causes cell damage by over-producing ROS or reducing oxidative defense, and binding to tubulin leads to cytoskeletal changes and inhibition of the Na + /Ka + pump and protein synthesis, eventually leading to impaired cell function or abnormal cell death (Hare and Atchison, 1994;Orrenius et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, MeHg could inhibit the synthesis of proteins associated with the development of embryo chorions, which also delay the development and hatching of fish (Cuello et al, 2012). In addition, MeHg exposure may induce oxidative stress, which constrain the function of energy metabolism and enzyme activities and eventually retards the hatching process (Gonzalez et al, 2005;Wu et al, 2018).…”

Section: Toxicity Of Mehg To Hatching Process Of Embryosmentioning

confidence: 99%

“…Apoptosis is accompanied by some biological processes such as cell death, gastrulation and tissue interaction which can lead to abnormal morphology of developing fish larvae to some extent (Orrenius et al, 2003). Finally, other factors such as MeHg-induced oxidative stress, inhibition of acetylcholinesterase activity, and influence of immune gene expression may also be responsible for the morphological abnormalities of fish (Wu et al, 2018). In addition, high deformity rates are often accompanied by low survival rates, suggesting that malformation in the ELSs is likely to increase larval mortality.…”

Section: Toxicity Of Mehg To the Growth And Development Of Embryos Anmentioning

confidence: 99%

“…Early life stages (ELSs) of fish are extremely sensitive to contaminants, low concentrations of contaminants may not be sufficiently poisonous to adult fish but it can cause harmful impacts on embryos and larvae (Jezierska et al, 2009). Numerous studies have confirmed that MeHg has adverse effects on the early development of fish, leading to growth retardation, increased mortality, impaired cardiac function, morphological abnormalities, genetic, and protein expression disorders (Hassan et al, 2012;Wu et al, 2018;Ren et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Effects of Short Term Methylmercury Exposure on Growth and Development of the Large Yellow Croaker Embryos and Larvae

et al. 2019

Front. Mar. Sci.

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Due to the high toxicity in aquatic food chain, the toxic effects of methylmercury (MeHg) on organisms have caused widespread concerns, but little has been reported on its effects on marine fish. The acute and sub-lethal toxicities of MeHg chloride to an important aquaculture species in southeast coast of China -large yellow croaker (Pseudosciaena crocea) embryos and larvae were investigated in this study. Acute toxicity tests showed that the 48-h LC 50 values of methylmercury to fish embryos and larvae were 28.39 (21.33-33.98) and 18.27 (11.33-29.29) µg L −1 , respectively. The 96-h LC 50 value for larvae was 9.28 (4.41-14.49) µg L −1 , which indicated that fish larvae were more sensitive to MeHg than the embryos. On the other hand, MeHg exposure induced delayed hatching process, lower yolk absorption rate, higher morphological malformations at concentrations ≥ 1 µg L −1 , and caused higher larvae heart rate, lower survival and hatching success at concentrations ≥ 5, 10, and 20 µg L −1 , respectively. The results suggested that the hatching, survival and growth of the large yellow croaker during the early life stages may serve as sensitive biomarkers for marine MeHg pollution.

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Effects of mercury chloride on antioxidant and inflammatory cytokines in zebrafish embryos

Bhai,

Binesh,

Shanmugam

et al. 2023

J Biochem & Molecular Tox

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In this study, a zebrafish embryo toxicity model was employed, utilizing 24 h postfertilization (hpf) zebrafish embryos. These embryos were treated with varying concentrations of mercuric chloride for 96 h under static conditions. We assessed multiple parameters that reflected developmental abnormalities, behavioral alterations, morphological anomalies, antioxidant enzyme activities, including those of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), and glutathione S‐transferase (GST), immune messenger RNA transcription levels of key factors such as tumor necrosis factor α (TNF‐α), interleukin‐1β (IL‐1β), interleukin‐6 (IL‐6), and cyclooxygenase 2 (COX‐2), as well as protein expression of TNF‐α. The results revealed that embryos exposed to higher concentrations of mercury exhibited reduced hatchability and increased rates of morphological abnormalities and mortality at 48, 72, and 96 hpf. In addition, a concentration‐dependent increase in developmental abnormalities, including cardiac edema, reduced body length, yolk sac edema, scoliosis, and bent tails, was observed. Larval behaviors, such as touch‐induced escape responses, startle reactions, and turning actions, were found to be diminished in a concentration‐dependent manner. Additionally, the activities of various antioxidative enzymes, such as SOD, CAT, and GST, exhibited an increase at higher mercury concentrations, with the exception of GPX activity, which decreased significantly in a dose‐dependent manner (p < 0.05). Pro‐inflammatory cytokine transcription levels, specifically TNF‐α, IL‐1β, IL‐6, and COX‐2, were significantly upregulated in a dose‐dependent manner in the mercuric (II) chloride (HgCl2) treatment group compared with the control group. TNF‐α protein expression was notably elevated in the larvae group treated with 300 and 400 nM HgCl2.

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Responses of Antioxidant Defense and Immune Gene Expression in Early Life Stages of Large Yellow Croaker (Pseudosciaena crocea) Under Methyl Mercury Exposure

Cited by 26 publications

References 59 publications

Dietary Aroclor 1254-Induced Toxicity on Antioxidant Capacity, Immunity and Energy Metabolism in Chinese Mitten Crab Eriocheir sinensis: Amelioration by Vitamin A

Dietary Aroclor 1254-Induced Toxicity on Antioxidant Capacity, Immunity and Energy Metabolism in Chinese Mitten Crab Eriocheir sinensis: Amelioration by Vitamin A

Effects of Short Term Methylmercury Exposure on Growth and Development of the Large Yellow Croaker Embryos and Larvae

Effects of mercury chloride on antioxidant and inflammatory cytokines in zebrafish embryos

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