Responses of antibiotic and heavy metal resistance genes to bamboo charcoal and bamboo vinegar during aerobic composting

Guo, Honghong; Gu, Jie; Wang, Xiaojuan; Yu, Jing; Nasir, Mubasher; Peng, Huiling; Zhang, Ranran; Hu, Ting; Wang, Qianzhi; Ma, Jiyue

doi:10.1016/j.envpol.2019.05.014

Cited by 78 publications

(23 citation statements)

References 48 publications

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“…After composting, the abundance of Actinomycetes increased in all four treatments, but the HT2C treatment had the least abundance of Actinomycetes. Actinobacteria have the function of carrying and spreading ARGs [ 39 ], which, on the one hand, explains the rebound of some ARGs at the end of composting [ 15 ] and, on the other hand, explains the better removal rate of some ARGs in HT2C. The main Actinomycetes that were identified during composting were Dyella and Streptomyces.…”

Section: Resultsmentioning

confidence: 99%

“…Some researchers found that zeolite that was added during composting reduced the total abundance of ARGs in the compost products [ 14 ]. Some researchers showed that the abundance of ARGs and MGEs decreased in the high-temperature period after adding bamboo charcoal and bamboo vinegar in the composting process [ 15 ]. Biochar, which is a kind of high-carbon material that is formed by high-temperature pyrolysis of biomass materials, is widely utilized in composting.…”

Section: Introductionmentioning

confidence: 99%

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Biochar and Hyperthermophiles as Additives Accelerate the Removal of Antibiotic Resistance Genes and Mobile Genetic Elements during Composting

Fu,

Zhang,

Guo

et al. 2021

Materials

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Sewage treatment plants are known as repositories of antibiotic resistance genes (ARGs). Adding biochar and inoculating with exogenous microbial agents are common ways to improve the quality of compost. However, little is known about the effects of these exogenous additives on the fate of ARGs during composting and the related mechanisms. In this study, municipal sludge was taken as the research object to study the ARG-removal effects of four composting methods: ordinary compost (CT), compost with hyperthermophiles (HT), compost with hyperthermophiles and 2.0% biochar (HT2C) and compost with hyperthermophiles and 5.0% biochar (HT5C). Real-time quantitative PCR (qPCR) and 16S rRNA high-throughput sequencing were conducted to analyze the ARGs, MGEs and bacterial community. After composting, the abundance of ARGs in CT was reduced by 72.7%, while HT, HT2C and HT5C were reduced by 80.7%, 84.3% and 84.8%, respectively. Treatments with different proportions of biochar added (HT2C, HT5C) had no significant effect on the abundance of ARGs. Network analysis showed that Firmicutes and Nitrospirae were positively associated with most ARGs and may be potential hosts for them. In addition, redundancy analysis further showed that the class 1 integrase gene (intI1), pH and organic carbon had a greater effect on ARGs. Our findings suggested that the combination of hyperthermophiles and biochar during the composting process was an effective way to control ARGs and mobile genetic elements (MGEs), thus inhibiting the spread and diffusion of ARGs in the environment and improving the efficiency of treating human and animal diseases.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Biochar and Hyperthermophiles as Additives Accelerate the Removal of Antibiotic Resistance Genes and Mobile Genetic Elements during Composting

Fu,

Zhang,

Guo

et al. 2021

Materials

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“…Psychrobacillus belongs to Firmicutes. A Previous study reported that Actinobacteria and Firmicutes were prevalent during the thermophilic phase of composting, and the prevalence of ARGs increased at the end of composting [41]. However, Solibacillus and Enterococcus of Firmicutes, Corynebacterium of Actinobacteria were significantly negatively correlated with various ARGs (Fig.…”

Section: Analysis Of Differences In Tcb and The Relationship Between Arb And Argsmentioning

confidence: 78%

Distribution of antibiotic-resistant bacteria in aerobic composting of swine manure with different antibiotics

Song

et al. 2021

Environ Sci Eur

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Background Livestock manure is an important reservoir of antibiotic-resistant bacteria (ARB) and antibiotic-resistance genes (ARGs). The bacterial community structure and diversity are usually studied using high-throughput sequencing that cannot provide direct evidence for ARB changes. Thus, little is known about the distribution of ARB, especially in the presence of different antibiotics in composting process. In this study, the fate of ARB was investigated in aerobic composting of swine manure, using chlortetracycline, sulfamethoxazole, lincomycin, and ciprofloxacin as typical antibiotics. The abundance and species of ARB were analyzed systematically to evaluate their ecological risk at different stages of composting. Results The absolute abundance of total ARB decreased, while the relative abundance increased on day 2. The relative abundance of lincomycin-resistant bacteria was higher than other ARBs during the whole composting process. The absolute abundance of four ARBs was 9.42 × 106–2.51 × 102 CFU/g (lincomycin- > chlortetracycline- > sulfamethoxazole- > ciprofloxacin- > multiple antibiotic-resistant bacteria), and they were not completely inactivated at the end of composting. Antibiotics led to a partial proliferation of ARBs including Corynebacterium, Sporosarcina, Solibacillus, and Acinetobacter. Especially, Corynebacterium, a pathogenic genus, was observed in chlortetracycline and lincomycin treatments. Conclusion Among the antibiotics studied, lincomycin showed the highest ecological risk, due to it expanded the range of lincomycin-resistant bacteria at the phyla level (Firmicutes, Actinobacteria, and Proteobacteria). The principal co-ordinates analysis indicated that the bacterial community structure was primarily associated with the composting stages rather than antibiotic types. Possible potential hosts and the related to the decrease of ARGs abundance were indicated based on the network analysis. The decrease of culturable Proteobacteria and the increase of culturable Firmicutes (Solibacillus, Bacillus) partially explained the high degradation rate of various ARGs with the progress of composting in this study. These results provided important information for the control of antibiotic resistance in composting.

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“…Psychrobacillus belongs to Firmicutes. A Previous study reported that Actinobacteria and Firmicutes were prevalent during the thermophilic phase of composting, and the prevalence of ARGs increased in the end of composting [41]. However, Solibacillus and Enterococcus of Firmicutes, Corynebacterium_1 of Actinobacteria were negatively correlated with various ARGs (Fig.…”

Section: Analysis Of Differences In Tcb and The Relationship Between Arb And Argsmentioning

confidence: 79%

Distribution of Antibiotic-resistant Bacteria in Aerobic Composting of Swine Manure With Different Antibiotics

Song

et al. 2021

Preprint

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Background: Livestock manure is an important reservoir of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs). The community structure and bacterial diversity are usually studied using high-throughput sequencing that cannot provide direct evidence for ARB changes. Thus, little is known about the distribution of ARB, especially in the presence of different antibiotics in composting process. In this study, the fate of ARB was investigated in aerobic composting of swine manure, using chlortetracycline, sulfamethoxazole, lincomycin, ciprofloxacin as typical antibiotics. The abundance and species of ARB were analyzed systematically to evaluate their ecological risk at different stages of composting. Results: The absolute abundance of total ARB decreased while the relative abundance showed an increasing trend on Day 2. The relative abundance of lincomycin-resistant bacteria was higher than other ARBs during the whole composting process. The absolute abundance of four ARBs was 9.42×106-2.51×102 CFU/g (lincomycin- > chlortetracycline- > sulfamethoxazole- > ciprofloxacin- > multiple antibiotic-resistant bacteria), and they were not completely inactivated at the end of composting. Antibiotics led to a partial proliferation of ARBs including Corynebacterium_1, Sporosarcina, Solibacillus, and Acinetobacter. Especially, Corynebacterium_1, a pathogenic bacterium, was observed in the treatments of chlortetracycline and lincomycin. Conclusion: Among the antibiotics studied, lincomycin showed the highest ecological risk, due to it expanded the range of lincomycin-resistant bacteria at the phyla level (Firmicutes, Actinobacteria, and Proteobacteria). The principal co-ordinates analysis indicated that the bacterial community structure was primarily associated with the composting stages rather than the antibiotic types. Possible potential hosts and degrading bacteria for ARGs were indicated based on the network analysis. The decrease of culturable Proteobacteria and the increase of culturable Firmicutes (Solibacillus, Bacillus) partially explained the high removal rate of various ARGs in this study. These results provided important information for the control of antibiotic resistance in composting.

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Responses of antibiotic and heavy metal resistance genes to bamboo charcoal and bamboo vinegar during aerobic composting

Cited by 78 publications

References 48 publications

Biochar and Hyperthermophiles as Additives Accelerate the Removal of Antibiotic Resistance Genes and Mobile Genetic Elements during Composting

Biochar and Hyperthermophiles as Additives Accelerate the Removal of Antibiotic Resistance Genes and Mobile Genetic Elements during Composting

Distribution of antibiotic-resistant bacteria in aerobic composting of swine manure with different antibiotics

Distribution of Antibiotic-resistant Bacteria in Aerobic Composting of Swine Manure With Different Antibiotics

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