Response to short term ultraviolet stress in the reef-building coral Pocillopora capitata

Liñán‐Cabello, Marco A.; Flores-Ramírez, Laura A.; Mena-Herrera, Alfredo; Cobo‐Díaz, José F.; Zenteno‐Savín, Tania; Olguín-Monroy, Norma O.

doi:10.1016/j.cbpa.2008.05.066

Cited by 3 publications

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“…It has previously been reported that specimens of P. capitata from LB, in addition to exhibiting short-term adjustments such as the amount of photosynthetic pigment and/or sensitivity to the photosynthetic response reported in other coral species, exhibit a series of short-term enzymatic responses aimed at countering the effects of ROS, thereby promoting adaptation to shallow coastal environments which typically exhibit high levels of UVR and thermal variability (Flores-Ramirez & Liñán-Cabello 2007, Liñán-Cabello et al 2010a). According to Freire et al (2011), changes in HSP (and other proteins) expression can be detected within a few hours (1-6 h), corresponding to the duration of most physiological events that occur in tidal cycles.…”

Section: Discussionmentioning

confidence: 99%

Response to pH stress in the reef-building coral Pocillopora capitata (Anthozoa: Scleractinia)

Delgadillo-Nuño

Liñán‐Cabello

Reyes-Gómez

et al. 2014

Rev. biol. mar. oceanogr.

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Resumen.-Para evaluar la respuesta metabólica del coral simbiótico Pocillopora capitata a la reducción del pH del agua de mar en un sistema in vitro, 112 ramas de P. capitata se obtuvieron de la comunidad de corales La Boquita (LB) sin exhibir algún daño aparente de blanqueamiento. Se evaluaron 2 tratamientos de pH: a) 7,85/7,95 pH (Tratamiento C 1 ), b) 7,60/7,70 (Tratamiento C 2 ) y 8,00/8,40 pH tratamiento de control (CC). Las ramas de coral fueron asignadas aleatoriamente a unidades experimentales (n= 38 por tratamiento). Las muestras fueron tomadas en 2 tiempos para el análisis bi oquímico y para evaluar cualitativamente los aspectos microestructurales por microscopía electrónica de barrido: tiempo inicial, muestra tomada en 12 h (T 1 ); tiempo final, muestras del último día de la exposición (T f , día 7 del experimento). A diferencia del tejido simbionte, el análisis bioquímico del tejido del coral reveló que P. capitata muestra una respuesta inmediata reflejada en los coeficientes de ARN/ADN y proteína/ADN, así como en las concentraciones de ARN y proteína, particularmente en el tratamiento de C 2 en las horas iniciales del experimento. El análisis microestructural cualitativo identificó principalmente efectos en el tratamiento C 2 , que fue influenciado por la presencia de disgregaciones micro-superficiales en las regiones terminales de las fibras esqueléticas. Palabras clave: Pocillopora capitata, coral, ARN/ADN, Proteina/ADN, fibras esqueléticas, estrésAbstract.-To evaluate the metabolic response of the symbiotic coral Pocillopora capitata to reduced seawater pH in an in vitro system, 112 branches of P. capitata with no apparent damage from bleaching were collected from the La Boquita (LB) coral community. Two pH treatments were evaluated: a) pH 7.85/7.95 (Treatment C 1 ), b) 7.60/7.70 (Treatment C 2 ), and 8.00/ 8.40 pH control treatment (CC). The coral branches were randomly assigned to experimental units (n= 38 per treatment). Samples were taken at 2 separate times for biochemical analysis and qualitative assessment of microstructural aspects with scanning electron microscopy: initial time, sample taken at 12 h (T 1 ); end time, samples from the final day of exposure (T f , day 7 of the experiment). Unlike the symbiont tissue, the biochemical analysis of the host tissue revealed that P. capitata displayed an immediate response as reflected in the RNA/DNA and protein/DNA ratios as well as in the concentrations of RNA and protein, particularly in the initial hours of the experiment in treatment C 2 . Qualitative microstructural analysis primarily identified effects in treatment C 2 which was influenced by the presence of micro surface detachments in the terminal regions of the skeletal fibers.

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Section: Discussionmentioning

confidence: 99%

Response to pH stress in the reef-building coral Pocillopora capitata (Anthozoa: Scleractinia)

Delgadillo-Nuño

Liñán‐Cabello

Reyes-Gómez

et al. 2014

Rev. biol. mar. oceanogr.

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“…Without such definition, there is potential to mistake significant variations in coral health for responses to stress exposure, rather than those due in-part to normal biological processes based on the timing of coral sampling with respect to baseline (Rougée et al, 2006). For example, there would be great importance and versatility in the use of CAT, GR, SOD, and GPx for defining ROS-induced stress in coral animals, which aids in better defining such information as seasonal stress variations, xenobiotic impacts, and thermal stress limitations in corals (Downs & Downs, 2007; Griffin, Bhagooli & Weil, 2006; Higuchi et al, 2008; Lesser, 1996; Liñán Cabello et al, 2010a; Liñán Cabello et al, 2010b). Work to characterize baseline homeostatic levels of activity of these enzymes will benefit the coral conservation biology community and aid in improving experimental design by accounting for changes in the background activity levels of antioxidant enzymes due to innate biological processes.…”

Section: Discussionmentioning

confidence: 99%

“…Understanding the influence of reproduction on various biomarker enzymes in coral remains a poorly characterized field that merits expansion. The enzymes employed in this study have been widely applied to evaluate the effect of many abiotic stressors on coral health (Flores-Ramírez & Liñán Cabello, 2007; Higuchi et al, 2008; Liñán Cabello et al, 2010a; Liñán Cabello et al, 2010b; Richier et al, 2003; Verma, Mehta & Srivastava, 2007; Yakovleva et al, 2004). However, failing to consider reproduction as a source of inherent variation in ROS-induced stress responses presents a potential confounder for studies using these biomarkers by potential mischaracterization of natural fluctuations in antioxidant biomarkers as stress responses.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have highlighted antioxidant enzymes as useful biomarkers of the impacts of stressors such as heat, xenobiotic exposure, and high-irradiance (Downs et al, 2006; Higuchi, Yuyama & Nakamura, 2015; Liñán Cabello et al, 2010a; Liñán Cabello et al, 2010b; Olsen et al, 2013). However, as useful as this suite of enzymes is in providing information about coral stress responses and threat levels, many of the substrates that trigger this type of stress are naturally produced in normal homeostatic processes (Agarwal, Gupta & Sikka, 2006; Dowling & Simmons, 2009; Fujii, Iuchi & Okada, 2005).…”

Section: Introductionmentioning

confidence: 99%

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Antioxidant enzyme cycling over reproductive lunar cycles inPocillopora damicornis

Murphy

Collier

Richmond

2019

PeerJ

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The impacts of continued degradation of watersheds on coastal coral reefs world-wide is alarming, and action addressing anthropogenic stressors and subsequent rehabilitation of watersheds and adjacent reefs is an urgent priority. The aim of this study is to develop and improve the use of antioxidant enzymes as bioindicators of stress in coral species. In order to fully develop such tools, it is necessary to first understand baseline cycling of these enzymes within coral tissues. Due to inherent links between reproduction and oxidative stress, these aims may be facilitated by sampling coral tissues over reproductively-linked lunar cycles to determine variations from baseline. By developing a greater understanding of biochemical markers of stress in corals, specifically antioxidant defense enzymes catalase (CAT), glutathione reductase (GR), glutathione peroxidase (GPx), and superoxide dismutase (SOD) in Hawaiian Pocillopora damicornis, we have provided molecular tools that identify thresholds of stress on coral reefs. Our results suggest that the coral reproductive state is a significant factor affecting the activity of antioxidant enzymes. Specifically, CAT and GR display maximum activity during peak reproductive state. Whereas significant maximal Se-independent GPx and SOD activity was measured during off-peak reproductive cycles. Such insight into the cyclical variation of the activity of these enzymes should be applied towards differentiating the influence of natural biological activity cycling in diagnostic tests identifying the effects of different physical environmental factors and chemical pollutants on coral health. Through the development and application of these molecular biomarkers of stress, we look to improve our ability to identify problems at the sub-lethal level, when action can be taken to mitigate a/biotic impacts.

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Ultraviolet radiation at Isla del Coco (Cocos Island) National Park, Costa Rica.

Vargas

Alfaro

2016

RBT

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Ultraviolet radiation at Isla del Coco (Cocos Island) National Park, Costa Rica. Incoming solar energy that reaches the land surface is divided mainly in infrared, visible and UV. UV radiation has wave lengths from 100 to 400 nm. The World Health Organization recommends studies and monitoring of UV radiation because of its biological effects in living organisms. The use of automatic Davis weather stations, Vantage Pro Plus/Vantage Pro2 Plus type, with a sensor model 6490, allowed UV radiation measurements at Isla del Coco, Costa Rica, located in the Eastern Tropical Pacific. The stations were set near Chatham Bay

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Response to short term ultraviolet stress in the reef-building coral Pocillopora capitata

Cited by 3 publications

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Response to pH stress in the reef-building coral Pocillopora capitata (Anthozoa: Scleractinia)

Response to pH stress in the reef-building coral Pocillopora capitata (Anthozoa: Scleractinia)

Antioxidant enzyme cycling over reproductive lunar cycles inPocillopora damicornis

Ultraviolet radiation at Isla del Coco (Cocos Island) National Park, Costa Rica.

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