Response to copper stress in aposymbiotically grown lichen mycobiont Cladonia cristatella: uptake, viability, ergosterol and production of non-protein thiols

Bačkor, Martin; Pawlik‐Skowrońska, Barbara; Tomko, Jaroslav; Buďová, Jana; Toppi, Luigi Sanità di

doi:10.1016/j.mycres.2006.05.007

Cited by 30 publications

(10 citation statements)

References 29 publications

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“…Ergosterol is known as the principal sterol of fungal plasma membranes. Its levels correlate with basal respiration rates of lichens (Sundberg et al, 1999) and decrease of ergosterol content due to short term (24 h) exposure to Cu excess in the aposymbiotically grown Cladonia cristatella mycobiont (Bačkor et al, 2006), or in the lichens Peltigera rufescens and C. arbuscula subsp. mitis were previously reported .…”

Section: Discussionmentioning

confidence: 95%

The presence of usnic acid does not protect the lichen Cladina arbuscula subsp. mitis against the long-term copper excess

Bačkorová

Biľová²,

Kimáková

et al. 2015

South African Journal of Botany

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a b s t r a c tCladina arbuscula subsp. mitis grows on the surfaces of historical Cu-mine spoil heaps rich in Cu, as well as on the surfaces of substrata that are not metal contaminated. This lichen contains the extracellular secondary metabolite, usnic acid (UA), which was previously found to form complexes with metal cations, including Cu. As UA can be experimentally removed from dry lichen thalli using acetone and without the loss of lichen viability, this study attempted to evaluate its role in long-term (8 wks) Cu tolerance in C. arbuscula subsp. mitis. Removal of UA by acetone did not affect the viability of the lichen, however it caused lower intracellular Cu accumulation after 8 wks of experiment at the highest 500 μM Cu doses tested. Thus far, we did not observe de novo synthesis of UA in the UA− variant of the study during the 8 wks of the experiment. Based on the measurements of the selected metabolic processes in the lichen (chlorophyll a fluorescence, chlorophyll a integrity, TBARS production, content of ergosterol and content of soluble proteins) it would seem that presence of UA in C. arbuscula subsp. mitis did not protect this lichen against Cu excess.

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Section: Discussionmentioning

confidence: 95%

The presence of usnic acid does not protect the lichen Cladina arbuscula subsp. mitis against the long-term copper excess

Bačkorová

Biľová²,

Kimáková

et al. 2015

South African Journal of Botany

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“…The impact of environmental stress on the mycobiont can be assessed by the amount of ergosterol, which is the principal sterol of fungal plasma membranes. Ergosterol levels were shown to be correlated with the basal respiration rate of lichens (Sundberg et al 1999) and its decrease a consequence of short-term exposure to Cu excess was found in aposymbiotically grown Cladonia cristatella mycobionts (Bačkor et al 2006b) and P. rufescens and C. arbuscula subsp. mitis ).…”

Section: Discussionmentioning

confidence: 97%

Physiological Aspects of Cadmium and Nickel Toxicity in the Lichens Peltigera rufescens and Cladina arbuscula Subsp. mitis

Bačkor¹,

Kováčik²,

Piovár³

et al. 2009

Water Air Soil Pollut

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This study was undertaken with the aim of investigating the effect of Cd 2+ and Ni 2+ containing solutions on selected physiological parameters (metal uptake, chlorophyll a fluorescence, assimilation pigment composition, thiobarbituric acid-reactive substance production, and ergosterol content) in the lichens Peltigera rufescens and Cladina arbuscula subsp. mitis growing on historic copper mine-spoil heaps at Ľubietová-Podlipa, Slovakia. Physiological measurements did not confirm significantly higher sensitivity to Cd and Ni of the cyanolichen P. rurescens compared to the green-algal lichen C. arbuscula subsp. mitis. Under natural conditions, C. arbuscula subsp. mitis is able to grow directly on copper mine heaps of Central Slovakia, while P. rufescens grows only on their margins. A crucial factor for this limited distribution of P. rufescens may be, at least in part, the higher intracellular accumulation of metals. Although lichen photobionts are generally regarded as key elements of lichen sensitivity, further research is necessary to elucidate this point since the higher levels of intracellular Cd and Ni do not allow to regard cyanobacterial photobionts of P. rufescens as more sensitive than the eukaryotic ones of C. arbuscula subsp. mitis.

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“…Ergosterol levels correlate with basal respiration rates of lichens (Sundberg et al 1999) and decrease due to short term (24 h) exposure to copper excess in the aposymbiotically grown Cladonia cristatella mycobiont (Bačkor et al 2006b). …”

Section: Discussionmentioning

confidence: 99%

Physiological comparison of copper toxicity in the lichens Peltigera rufescens (Weis) Humb. and Cladina arbuscula subsp. mitis (Sandst.) Ruoss

Bačkor¹,

Kováčik²,

Dzubaj³

et al. 2009

Plant Growth Regul

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Response to copper stress in aposymbiotically grown lichen mycobiont Cladonia cristatella: uptake, viability, ergosterol and production of non-protein thiols

Cited by 30 publications

References 29 publications

The presence of usnic acid does not protect the lichen Cladina arbuscula subsp. mitis against the long-term copper excess

The presence of usnic acid does not protect the lichen Cladina arbuscula subsp. mitis against the long-term copper excess

Physiological Aspects of Cadmium and Nickel Toxicity in the Lichens Peltigera rufescens and Cladina arbuscula Subsp. mitis

Physiological comparison of copper toxicity in the lichens Peltigera rufescens (Weis) Humb. and Cladina arbuscula subsp. mitis (Sandst.) Ruoss

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