Response to Comment on Pilemann-Lyberg et al. Uric Acid Is an Independent Risk Factor for Decline in Kidney Function, Cardiovascular Events, and Mortality in Patients With Type 1 Diabetes. Diabetes Care 2019;42:1088–1094

Pilemann-Lyberg, Sascha; Hansen, Tine W.; Rossing, Peter

doi:10.2337/dci19-0037

Cited by 2 publications

(2 citation statements)

References 5 publications

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“…Uric acid is mainly excreted by the kidney, and a small amount is excreted by the gastrointestinal tract. Insufficient excretion of uric acid can cause kidney damage ( Pilemann-Lyberg et al, 2019 ). As the creatinine (Cr) and blood urea nitrogen (BUN) levels in serum are effective indicators of renal function, the levels of Cr and BUN in the serum of each group were detected by detection kit.…”

Section: Resultsmentioning

confidence: 99%

Pharmacological Basis for Use of a Novel Compound in Hyperuricemia: Anti-Hyperuricemic and Anti-Inflammatory Effects

et al. 2021

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Background: The prevalence of hyperuricemia is considered high worldwide. Hyperuricemia occurs due to decreased excretion of uric acid, increased synthesis of uric acid, or a combination of both mechanisms. There is growing evidence that hyperuricemia is associated with a decline of renal function.Purpose: This study is aimed at investigating the effects of the novel compound on lowering the serum uric acid level and alleviating renal inflammation induced by high uric acid in hyperuricemic mice.Methods: Hyperuricemic mice model was induced by potassium oxonate and used to evaluate the effects of the novel compound named FxUD. Enzyme-linked immunosorbent assay was used to detect the related biochemical markers. Hematoxylin-eosin (HE) staining was applied to observe pathological changes. The mRNA expression levels were tested by qRT-PCR. The protein levels were determined by Western blot. In parallel, human proximal renal tubular epithelial cells (HK-2) derived from normal kidney was used to further validate the anti-inflammatory effects in vitro.Results: FxUD administration significantly decreased serum uric acid levels, restored the kidney function parameters, and improved the renal pathological injury. Meanwhile, treatment with FxUD effectively inhibited serum and liver xanthine oxidase (XOD) levels. Reversed expression alterations of renal inflammatory cytokines, urate transporter 1 (URAT1) and glucose transporter 9 (GLUT9) were observed in hyperuricemic mice. Western blot results illustrated FxUD down-regulated protein levels of inflammasome components. Further studies showed that FxUD inhibited the activation of NF-κB signaling pathway in the kidney of hyperuricemic mice. In parallel, the anti-inflammatory effect of FxUD was also confirmed in HK-2.Conclusion: Our study reveals that FxUD exhibits the anti-hyperuricemic and anti-inflammatory effects through regulating hepatic XOD and renal urate reabsorption transporters, and suppressing NF-κB/NLRP3 pathway in hyperuricemia. The results provide the evidence that FxUD may be potential for the treatment of hyperuricemia with kidney inflammation.

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Section: Resultsmentioning

confidence: 99%

Pharmacological Basis for Use of a Novel Compound in Hyperuricemia: Anti-Hyperuricemic and Anti-Inflammatory Effects

et al. 2021

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“…Several epidemiological studies have suggested a positive relationship between elevated serum uric acid levels and an increased risk of cardiovascular events, such as coronary heart disease, stroke, and heart failure (Hozawa et al, 2006; Keenan et al, 2016; Kim et al, 2009). Even after controlling for other risk factors, elevated uric acid remained an independent risk factor for cardiovascular events in type 1 diabetes patients with hypertension, diabetes, and preexisting cerebrovascular and cardiovascular illness (Pilemann‐Lyberg et al, 2019). But it is unclear whether elevated uric acid levels are a cause or a consequence of IS.…”

Section: Discussionmentioning

confidence: 99%

Investigating the in vivo effect of Tribulus terrestris extract in middle cerebral artery occlusion rats using LC–MS‐based metabolomics combined with molecular docking

Guo

Zhao

et al. 2023

Biomedical Chromatography

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Tribulus terrestris L. fruit (TT) is a traditional Chinese herbal medicine used to treat ischemic stroke (IS). This study aimed to investigate the protective effect of TT extract, named TT15, on middle cerebral artery occlusion (MCAO) rats using metabolomics and molecular docking and find the targets of action and the material basis of TT15 against IS. The results of the infarct volume and neurological defect scores confirmed the efficacy of TT15. Serum metabolomics analysis using LC-MS revealed that model group animals experienced a variety of metabolic disturbances when compared to the sham group. TT15 can restore the MCAO-induced serum metabolite changes by modulating multiple metabolic pathways. Six enzymes were highlighted by the metabolite-reaction-enzyme-gene (M-R-E-G) network analysis, which might be the possible targets for the TT15 against IS. Molecular docking analysis was applied to show the binding affinities between active compounds and these enzymes.The representative docking mode with the lowest binding energy between three compounds and phospholipase A 2 (PLA2) and peroxidase (POD) was displayed by the ribbon binding map. This study profiles the metabolic changes in MCAO-induced IS and investigates the efficacy and the corresponding mechanism of TT15 in the treatment of IS.

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Response to Comment on Pilemann-Lyberg et al. Uric Acid Is an Independent Risk Factor for Decline in Kidney Function, Cardiovascular Events, and Mortality in Patients With Type 1 Diabetes. Diabetes Care 2019;42:1088–1094

Cited by 2 publications

References 5 publications

Pharmacological Basis for Use of a Novel Compound in Hyperuricemia: Anti-Hyperuricemic and Anti-Inflammatory Effects

Pharmacological Basis for Use of a Novel Compound in Hyperuricemia: Anti-Hyperuricemic and Anti-Inflammatory Effects

Investigating the in vivo effect of Tribulus terrestris extract in middle cerebral artery occlusion rats using LC–MS‐based metabolomics combined with molecular docking

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