Response to Challenge withEdwardsiella ictaluriby Channel Catfish,Ictalurus punctatus,Selected for Resistance toE. ictaluri

Dunham, Rex A.; Brady, Yolanda J.; Vinitnantharat, Somsak

doi:10.1300/j028v03n03_01

Cited by 30 publications

(13 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To create resource cDNA libraries containing a full complement of gene transcripts, including those expressed after infection, both healthy and infected catfish were used. Channel catfish and blue catfish were challenged with Edwardsiella ictaluri using procedures adapted from Dunham et al [42]. Fish were divided into 2 groups, the non-challenged controls and the fish for challenge (N = 240).…”

Section: Methodsmentioning

confidence: 99%

Towards the ictalurid catfish transcriptome: generation and analysis of 31,215 catfish ESTs

Peatman

Wang

et al. 2007

BMC Genomics

Self Cite

View full text Add to dashboard Cite

Background: EST sequencing is one of the most efficient means for gene discovery and molecular marker development, and can be additionally utilized in both comparative genome analysis and evaluation of gene duplications. While much progress has been made in catfish genomics, large-scale EST resources have been lacking. The objectives of this project were to construct primary cDNA libraries, to conduct initial EST sequencing to generate catfish EST resources, and to obtain baseline information about highly expressed genes in various catfish organs to provide a guide for the production of normalized and subtracted cDNA libraries for large-scale transcriptome analysis in catfish.

show abstract

Section: Methodsmentioning

confidence: 99%

Towards the ictalurid catfish transcriptome: generation and analysis of 31,215 catfish ESTs

Peatman

Wang

et al. 2007

BMC Genomics

Self Cite

View full text Add to dashboard Cite

show abstract

“…Challenge experiments were conducted as previously described [31] with modifications [32,33]. Briefly, catfish were challenged in a rectangular tank by immersion exposure for 2 h with freshly prepared culture of ESC bacteria, Edwardsiella ictaluri.…”

Section: Cmentioning

confidence: 99%

Characterization of a NK-lysin antimicrobial peptide gene from channel catfish

Wang

Bao

Wang

et al. 2006

Fish & Shellfish Immunology

View full text Add to dashboard Cite

“…Challenge experiments were conducted as previously described (Dunham et al 1993) with modifications. Briefly, catfish were challenged in a rectangular tank by immersion exposure for 2 h with freshly prepared culture of ESC bacteria, E. ictaluri.…”

Section: Fish Rearing Bacterial Challenge and Samplingmentioning

confidence: 99%

Catfish CC chemokines: genomic clustering, duplications, and expression after bacterial infection with Edwardsiella ictaluri

Peatman

Bao

et al. 2005

Mol Genet Genomics

Self Cite

View full text Add to dashboard Cite

Chemokines are a family of structurally related chemotactic cytokines that regulate the migration of leukocytes, under both physiological and inflammatory conditions. CC chemokines represent the largest subfamily of chemokines with 28 genes in mammals. Sequence conservation of chemokines between teleost fish and higher vertebrates is low and duplication and divergence may have occurred at a significantly faster rate than in other genes. One feature of CC chemokine genes known to be conserved is genomic clustering. CC chemokines are highly clustered within the genomes of human, mouse, and chicken. To exploit knowledge from comparative genome analysis between catfish and higher vertebrates, here we mapped to bacterial artificial chromosome (BAC) clones 26 previously identified catfish (Ictalurus sp.) chemokine cDNAs. Through a combination of hybridization and fluorescent fingerprinting, 18 fingerprinted contigs were assembled from BACs containing catfish CC chemokine genes. The catfish CC chemokine genes were found to be not only highly clustered in the catfish genome, but also extensively duplicated at various levels. Comparisons of the syntenic relationships of CC chemokines may help to explain the modes of duplication and divergence that resulted in the present repertoire of vertebrate CC chemokines. Here we have also analyzed the expression of the transcripts of the 26 catfish CC chemokines in head kidney and spleen in response to bacterial infection of Edwardsiella ictaluri, an economically devastating catfish pathogen. Such information should pinpoint research efforts on the CC chemokines most likely involved in inflammatory responses.

show abstract

Response to Challenge withEdwardsiella ictaluriby Channel Catfish,Ictalurus punctatus,Selected for Resistance toE. ictaluri

Cited by 30 publications

References 0 publications

Towards the ictalurid catfish transcriptome: generation and analysis of 31,215 catfish ESTs

Towards the ictalurid catfish transcriptome: generation and analysis of 31,215 catfish ESTs

Characterization of a NK-lysin antimicrobial peptide gene from channel catfish

Catfish CC chemokines: genomic clustering, duplications, and expression after bacterial infection with Edwardsiella ictaluri

Contact Info

Product

Resources

About