Response surface optimization of selenium-enriched Moringa oleifera seed peptides with antioxidant, ACEI and XOI activities

In this study, five novel Se-enriched antioxidant peptides (FLSeML, LSeMAAL, LASeMMVL, SeMLLAA, and LSeMAL) were purified and identified from Se-enriched Moringa oleifera (M. oleifera) seed protein hydrolysate. The five peptides showed excellent cellular antioxidant activity, with respective EC50 values of 0.291, 0.383, 0.662, 0.1, and 0.123 μg/mL. The five peptides (0.025 mg/mL) increased the cell viability from 78.72 to 90.71, 89.16, 93.92, 83.68, and 98.29%, respectively, effectively reducing reactive oxygen species accumulation and significantly increasing superoxide dismutase and catalase activities in damaged cells. Molecular docking results revealed that the five novel Se-enriched peptides interacted with the key amino acid of Keap1, thus directly blocking the interaction of Keap1-Nrf2 and activating the antioxidant stress response to enhance the ability of scavenging free radicals in vitro. In conclusion, Se-enriched M. oleifera seed peptides exhibited significant antioxidant activity and can be expected to find widespread use as a highly active natural functional food additive and ingredient.

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“…On the basis of previous studies, Se-enriched M. oleifera seed protein hydrolysate was prepared via pepsin hydrolysis (37 °C).…”

Section: Methodsmentioning

confidence: 99%

Purification, Identification, and Mechanistic Investigation of Novel Selenium-Enriched Antioxidant Peptides from Moringa oleifera Seeds

Chen

Miao

et al. 2023

J. Agric. Food Chem.

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“…The results suggested that MRPs obtained from the mixture of salmon frame hydrolysate and Glx could serve as radical scavenger and thus could be considered as primary antioxidant. The DPPH assay has been utilised as a method to assess the antioxidant activity of substances by measuring their capability to act as hydrogen donors or free radical scavengers (Chen et al ., 2023). DPPH, existing as a stable free radical in ethanol, exhibits its peak absorbance at 517 nm.…”

Section: Resultsmentioning

confidence: 99%

Debittering of salmon frame protein hydrolysate and plastein using Maillard reaction as affected by types of sugar

Sharma,

Nilsuwan,

Hong

et al. 2024

Int J of Food Sci Tech

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SummaryImpacts of Maillard reaction on bitterness, physicochemical properties, antioxidant activities of salmon frame protein hydrolysates (PH) and its plastein (PT) using ribose or glucosamine (Glx) were investigated. PT showed higher bitterness than PH after Maillard reaction, regardless of sugars and concentrations used (P < 0.05). PH (10%) and 4% Glx heated at 120 °C for 120 min yielded Maillard reaction products (MRPs) with the least bitterness (score: 5.89) as compared to control sample (P < 0.05). Browning intensity and lightness of aforesaid sample were directly and inversely proportional to treatment time, respectively. MRPs (10% PH with 4% Glx) showed the increased DPPH and ABTS radical scavenging activities (37.06 and 45.65 μmol TE g−1, respectively) up to 60 min (P < 0.05). Moreover, bitterness score of MRPS heated for 60 min was reduced from 9.37 (PH) to 1.44 ascertained by decrease in hydrophobic amino acid contents (P < 0.05). Therefore, Maillard reaction could be used as a promising means to lower bitterness of salmon frame PH.

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Green synthesis of selenium nanoparticles using leaf extract of Moringa oleifera, their biological applications, and effects on the growth of Phaseolus vulgaris-: Agricultural synthetic biotechnology for sustainable nutrition

Banerjee,

Rajeswari

2024

Biocatalysis and Agricultural Biotechnology

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Response surface optimization of selenium-enriched Moringa oleifera seed peptides with antioxidant, ACEI and XOI activities

Cited by 6 publications

References 58 publications

Purification, Identification, and Mechanistic Investigation of Novel Selenium-Enriched Antioxidant Peptides from Moringa oleifera Seeds

Purification, Identification, and Mechanistic Investigation of Novel Selenium-Enriched Antioxidant Peptides from Moringa oleifera Seeds

Debittering of salmon frame protein hydrolysate and plastein using Maillard reaction as affected by types of sugar

Green synthesis of selenium nanoparticles using leaf extract of Moringa oleifera, their biological applications, and effects on the growth of Phaseolus vulgaris-: Agricultural synthetic biotechnology for sustainable nutrition

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