Response Surface Optimization of Bioactive Compounds Extraction from Buchholzia coriacea Using D-Optimal Design

Abstract

This study investigates the use of D-optimal design and response surface methodology for the optimization of bioactive compound extraction from Buchholzia Coriacea. After thorough sample preparation, the Soxhlet apparatus was employed to extract the bioactive compound from the dried Buchholzia coriacea seed in a batch-wise process. The D-optimal design was employed to generate 23 sets of experimental runs to determine the effect of temperature, extraction time, and the weight of the dried sample on the extraction yield. The temperature, extraction time, and the weight of the dried sample were found to significantly influence the extraction yield resulting in a maximum value of 41%. Optimum conditions of 80 o C, 20 g, and 50 min representing temperature, the weight of the dried sample, and extraction time were obtained using the desirability analysis.