Response surface modeling the significance of nitrogen source on the cell growth and Tuber polysaccharides production by submerged cultivation of Chinese truffle Tuber sinense

Liu, Rui-Sang; Li, Dong‐Sheng; Li, Hongmei; Tang, Ya‐Jie

doi:10.1016/j.procbio.2008.04.009

Cited by 47 publications

(28 citation statements)

References 17 publications

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“…Carbon sources are major components of the nutrient medium, which must ensure the best growth of fungi. Submerged culture has a number of advantages including higher mycelial yield in a more compact space and shorter time, with fewer chances of contamination [9][10][11] which is now attracting attention as an alternative for efficient yield of mycelia and polysaccharide [12]. The mycelial growth of edible mushroom using different carbon and nitrogen source under submerged culture condition was tested using basal synthetic media.…”

Section: Resultsmentioning

confidence: 99%

Growth of mycelial biomass and fruit body cultivation of Lentinus squarrosulus collected from home garden of Tripura in Northeast India

2015

J App Biol Biotech

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The present study aimed at cultivating the fruiting body of an edible mushroom identified as Lentinus squarrosulus collected from home garden of Tripura in Northeast India and for assessing the mycelium biomass growth using various carbon and nitrogen sources under submerged culture condition. The cultivation of the mushroom using paddy straw revealed dry weight of 0.25±0.02 g per fruit body with 21.83 % of biological efficiency. Under submerged culture condition, fructose exhibited highest mycelium dry weight and yeast extract was found to be a best nitrogen source. The availability of the mushroom in the forest and homegarden of Tripura may be utilized for its commercial cultivation and inclusion in the local food. Fructose and yeast extract is recommended for high mycelial biomass production.

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Section: Resultsmentioning

confidence: 99%

Growth of mycelial biomass and fruit body cultivation of Lentinus squarrosulus collected from home garden of Tripura in Northeast India

2015

J App Biol Biotech

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“…The potato dextrose agar slant and preparation of the preculture were previously described (24,33,34). Liquid seed medium consisted of glucose at 30 g/liter, NaNO 3 at 2 g/liter, K 2 HPO 4 at 1 g/liter, KCl at 0.5 g/liter, MgSO 4 ⅐ 7H 2 O at 0.5 g/liter, and FeSO 4 at 0.01 g/liter, and the pH was adjusted to 6.6 with 1.0 M HCl.…”

Section: Methodsmentioning

confidence: 99%

Novel Tandem Biotransformation Process for the Biosynthesis of a Novel Compound, 4-(2,3,5,6-Tetramethylpyrazine-1)-4′-Demethylepipodophyllotoxin

Tang

Zhao

2011

Appl Environ Microbiol

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According to the structure of podophyllotoxin and its structure-function relationship, a novel tandem biotransformation process was developed for the directional modification of the podophyllotoxin structure to directionally synthesize a novel compound, 4-(2,3,5,6-tetramethylpyrazine-1)-4-demethylepipodophyllotoxin (4-TMP-DMEP). In this novel tandem biotransformation process, the starting substrate of podophyllotoxin was biotransformed into 4-demethylepipodophyllotoxin (product 1) with the demethylation of the methoxyl group at the 4 position by Gibberella fujikuroi SH-f13, which was screened out from Shennongjia prime forest humus soil (Hubei, China). 4-Demethylepipodophyllotoxin (product 1) was then biotransformed into 4-demethylpodophyllotoxone (product 2) with the oxidation of the hydroxyl group at the 4 position by Alternaria alternata S-f6, which was screened out from the gathered Dysosma versipellis plants in the Wuhan Botanical Garden, Chinese Academy of Sciences. Finally, 4-demethylpodophyllotoxone (product 2) and ligustrazine were linked with a transamination reaction to synthesize the target product 4-TMP-DMEP (product 3) by Alternaria alternata S-f6. Compared with podophyllotoxin (i.e., a 50% effective concentration [EC 50 ] of 529 M), the EC 50 of 4-TMP-DMEP against the tumor cell line BGC-823 (i.e., 0.11 M) was significantly reduced by 5,199 times. Simultaneously, the EC 50 of 4-TMP-DMEP against the normal human proximal tubular epithelial cell line HK-2 (i.e., 0.40 M) was 66 times higher than that of podophyllotoxin (i.e., 0.006 M). Furthermore, compared with podophyllotoxin (i.e., log P ‫؍‬ 0.34), the water solubility of 4-TMP-DMEP (i.e., log P ‫؍‬ 0.66) was significantly enhanced by 94%. For the first time, the novel compound 4-TMP-DMEP with superior antitumor activity was directionally synthesized from podophyllotoxin by the novel tandem biotransformation process developed in this work.Podophyllotoxin (PTOX) is a naturally occurring aryltetralin lignan, and interest in it has been heightened by its potential antitumor activity (3, 16) but has failed to be used in human clinical trials because of unacceptable gastrointestinal toxic side effects (3). In order to overcome these limitations, numerous studies of Podophyllum lignans currently focus on the structural modification of the cycloparaffin (C ring) in the tetranap skeleton and the benzene with three methoxyl groups (E ring) to generate derivatives with superior pharmacological profiles (25). Aromatic heterocyclic compounds substituted with a carbon-nitrogen (CON) bond at the 4 position (14) may be a very effective pathway for improving the antitumor activity of PTOX, and most of these derivatives were more active than etoposide (1,5,7,8,15,20), which is a clinically important anticancer drug. Furthermore, previous investigations indicated that a PTOX derivative in which the para-position methoxyl group (4Ј-MeO) of PTOX was demethylated shows stronger anticancer activity and solubility than PTOX (29, 39). All of these analyses suggested that...

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“…for enzyme production (Saravanakumar et al, 2010) and Filamentous Fungi for Extracellular Lipase Production (Gaurav et al, 2017). Nonetheless, statistical models for research into the optimization of biomass production or metabolites by ectomycorrhizal fungi have scarcely been used (Srinivasan et al, 2000;Liu et al, 2008). For these, several selection criteria are required, particularly when the ultimate goal is the production of large amounts of biomass for the inoculation of plants on a large scale.…”

Section: Optimizationmentioning

confidence: 99%

Optimization of Biomass Production of Two Ectomycorrhizal Fungi (Lactarius quieticolor and Rhizopogon roseolus) for the Future Pinus radiata Nursery Inoculation

Chávez¹,

Machuca²,

Aguirre³

2017

Int.J.Curr.Res.Aca.Rev.

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Article InfoThe growing interest in mycelial biomass for its application in nurseries has led to the creation of inoculum production programs in New Zealand, Australia, the United States, and Chile, where the advantages of mycorrhizal inoculation have been demonstrated in plant morphological and physiological parameters. However, the mycelial biomass yields produced with these fungi through traditional submerged culture are generally low. Therefore, the aim of this study was to optimize the variables pH, temperature, and carbon source concentration required for maximum biomass production by the ectomycorrhizal fungi L. quieticolor and R. roseolus in liquid culture medium. The Box-Behnken (BB) design and response surface methodology (RSM) were used to establish the optimal conditions in the experimental area. Increases in biomass production of 1.2 g L -1 for 3.25 g L -1 for L. quieticolor (pH 5.5, 24°C and 20 g L -1 glucose) and 3.02 for 8.6 g L -1 for R. roseolus(pH 5.5, 28°C and 30 g L -1 mannitol) were found in comparison to the control culture (pH 5.5, 24°C and 10 g L -1 glucose) for both fungi. This optimization is the first step required to scale biomass production by L. quieticolor and R. roseolus in bioreactors.

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Response surface modeling the significance of nitrogen source on the cell growth and Tuber polysaccharides production by submerged cultivation of Chinese truffle Tuber sinense

Cited by 47 publications

References 17 publications

Growth of mycelial biomass and fruit body cultivation of Lentinus squarrosulus collected from home garden of Tripura in Northeast India

Growth of mycelial biomass and fruit body cultivation of Lentinus squarrosulus collected from home garden of Tripura in Northeast India

Novel Tandem Biotransformation Process for the Biosynthesis of a Novel Compound, 4-(2,3,5,6-Tetramethylpyrazine-1)-4′-Demethylepipodophyllotoxin

Optimization of Biomass Production of Two Ectomycorrhizal Fungi (Lactarius quieticolor and Rhizopogon roseolus) for the Future Pinus radiata Nursery Inoculation

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