Response surface methodology for the optimization of keratinase production in culture medium containing feathers produced by <i>Kocuria rosea</i>

Bernal, Cristobal; Dı́az, Isabel; Coello, Nereida

doi:10.1139/w05-139

Cited by 45 publications

(21 citation statements)

References 17 publications

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“…More recently, the use of statistical optimization by response surface methodology have been described for the production of bacterial keratinases during growth on raw feathers (Ramnani and Gupta 2004;Bernal et al 2006b). Factors like temperature and media composition significantly influence the production of keratinases, which could be improved nearly 40-fold after optimization.…”

Section: Bioprocessing Keratin-rich Wastesmentioning

confidence: 99%

Bacterial Keratinases: Useful Enzymes for Bioprocessing Agroindustrial Wastes and Beyond

Brandelli

2007

Food Bioprocess Technol

274

154

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Keratin-rich wastes in the form of feathers, hair, nails, and horn are highly available as byproducts of agroindustrial processing. The increased needs for energy conserving and recycling, summed with the huge increase in poultry industry, have strongly stimulated the search for alternatives for the management of recalcitrant keratinous wastes. Keratinases, which are produced by several bacteria that have been often isolated from soils and poultry wastes, show potential use in biotechnological processes involving keratin hydrolysis. Although these isolates are mostly restricted to the genera Streptomyces and Bacillus, the diversity of keratinolytic bacteria is significantly greater. Bacterial keratinases are mostly serine proteases, although increased information about keratinolytic metalloproteases, particularly from Gram-negative bacteria, became available. These enzymes are useful in processes related with the bioconversion of keratin waste into feed and fertilizers. Other promising applications have been associated with keratinolytic enzymes, including enzymatic dehairing for leather and cosmetic industry, detergent uses, and development of biopolymers from keratin fibers. The use of keratinases to enhance drug delivery in some tissues and hydrolysis of prion proteins arise as novel outstanding applications for these enzymes.

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Section: Bioprocessing Keratin-rich Wastesmentioning

confidence: 99%

Bacterial Keratinases: Useful Enzymes for Bioprocessing Agroindustrial Wastes and Beyond

Brandelli

2007

Food Bioprocess Technol

274

154

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“…High expression of the cloned apr E gene in B. subtilis cells, high keratin capability of these cells and the unutilized abundant amounts of sheep wool waste all greatly address the indispensable need for a step wise optimization of sheep wool biodegradation. Moreover, each microbial strain has its own peculiar physicochemical and nutritional requirements (Puri et al 2002;Bernal et al 2006) and no uniform defined medium for keratin biodegradation is reported till now. Improving keratin biodegradation is a prerequisite for designing biotechnological processes through developing efficient genetically modified bacterial strains and optimizing the media composition of the producers (Greasham 1983).…”

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confidence: 99%

Key determinants affecting sheep wool biodegradation directed by a keratinase-producing Bacillus subtilis recombinant strain

2010

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OVAT (one variable at a time) approach was applied in this study to screen the most important physicochemical key determinants involved in the process of sheep wool biodegradation. The process was directed by a keratinase-producing Bacillus subtilis DB 100 (p5.2) recombinant strain. Data indicate that, sheep wool could be degraded efficiently in cultures incubated at 30°C, with initial pH of 7 with agitation at 150 rpm. Two times autoclaved alkali treated and undefatted chopped sheep wool is more accessible to biodegradation. B. subtilis recombinant cells could utilize sheep wool as a sole source of carbon and nitrogen. Sheep wool-based modified basal medium II, lacking NH₄Cl and yeast extract, could greatly support the growth of these bacterial cells. Sheep wool biodegradation was conducted efficiently in the absence of kanamycin consequently; high stability of the recombinant plasmid (p5.2) represents a great challenge upon scaling up this process. Three key determinants (sheep wool concentration, incubation time and inoculum size) imposing considerable constraints on the process are highlighted. Sheep wool-based tap water medium and sheep wool-based distilled water medium were formulated in this study. High levels of released end products, produced from sheep wool biodegradation are achieved upon using these two sheep wool-based water media. Data indicate that, sheep wool hydrolysate is rich in some amino acids, such as tyrosine, phenylalanine, lysine, proline, isoleucine, leucine, valine, aspartic acid and glutamic acid. Moreover, the resulting sheep wool hydrolysate contains soluble proteins of high and intermediate molecular weights. The present study demonstrates a feasible, cheap, reproducible, efficient and rapid biotechnological approach towards utilization of raw sheep wool waste through a recombinant bacterium.

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“…The most significant parameters that affect keratinase production can be investigated using a onefactor-at-a-time method. Optimisation of the selected components can be achieved using a statistical approach such as employing the Plackett-Burman design and response surface methodology (RSM) to develop a mathematical model to identify the optimum conditions for higher keratinase production (Tiwary and Gupta, 2010;Pillai et al 2011;Rai and Mukerjee 2011;Haddar et al 2010;Bernal et al 2006b;Tatineni et al 2007;Embaby et al 2010)). …”

Section: Optimisation Of Keratinase Productionmentioning

confidence: 99%

Microbial keratinases: characteristics, biotechnological applications and potential.

Purchase

2016

The Handbook of Microbial Bioresources

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Keratinases are a group of proteolytic enzymes that can catalyse the cleavage and hydrolysis of the highly stable and fibrous proteins: keratins. A diverse range of microorganisms, including fungi, actinomycetes and bacteria, have been reported to produce keratinases that have biotechnological applications and potential. These keratinases have been usefully applied in agricultural, pharmaceutical, leather and textile processes as well as within environmentally friendly waste management solutions. Potential uses of keratinases include the fields of biomedicine, cosmetics, biological control and the generation of green energy. Herein we aim to provide an overview of the properties of this group of versatile enzymes, including the mechanisms of keratin degradation. The diversity of microbial sources of keratinases is discussed and the optimisation of keratianse production examined.We conclude with an assessment of the established biotechnological applications of keratinases in different industries and current research that highlights other promising potential uses.

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Response surface methodology for the optimization of keratinase production in culture medium containing feathers produced by Kocuria rosea

Cited by 45 publications

References 17 publications

Bacterial Keratinases: Useful Enzymes for Bioprocessing Agroindustrial Wastes and Beyond

Bacterial Keratinases: Useful Enzymes for Bioprocessing Agroindustrial Wastes and Beyond

Key determinants affecting sheep wool biodegradation directed by a keratinase-producing Bacillus subtilis recombinant strain

Microbial keratinases: characteristics, biotechnological applications and potential.

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