Response surface methodological approach for the synthesis of isobutyl isobutyrate

Hamsaveni, D.R; Prapulla, S. G.; Divakar, Soundar

doi:10.1016/s0032-9592(01)00142-x

Cited by 139 publications

(52 citation statements)

References 9 publications

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“…Many lipases are active in organic solvents where they catalyze a number of useful reactions including esterification (Chowdary et al, 2001;Hamsaveni et al, 2001;Kiran et al, 2001a;Kiyota et al, 2001;Rao and Divakar, 2001), transesterification, regioselective acylation of glycols and menthols, and synthesis of peptides (Ducret et al, 1998;Zhang et al, 2001) and other chemicals (Therisod and Klibanov, 1987;Weber et al, 1999;Bornscheuer, 2000;Berglund and Hutt, 2000;Liese et al, 2000;Azim et al, 2001). The expectation is that lipases will be as important industrially in the future as the proteases and carbohydrases are currently.…”

Section: Introductionmentioning

confidence: 99%

Production, purification, characterization, and applications of lipases

Sharma

Chisti

Banerjee

2001

Biotechnology Advances

1,255

712

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Lipases (triacylglycerol acylhydrolases, EC 3.1.1.3) catalyze the hydrolysis and the synthesis of esters formed from glycerol and long-chain fatty acids. Lipases occur widely in nature, but only microbial lipases are commercially significant. The many applications of lipases include speciality organic syntheses, hydrolysis of fats and oils, modification of fats, flavor enhancement in food processing, resolution of racemic mixtures, and chemical analyses. This article discusses the production, recovery, and use of microbial lipases. Issues of enzyme kinetics, thermostability, and bioactivity are addressed. Production of recombinant lipases is detailed. Immobilized preparations of lipases are discussed. In view of the increasing understanding of lipases and their many applications in high-value syntheses and as bulk enzymes, these enzymes are having an increasing impact on bioprocessing. D

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Section: Introductionmentioning

confidence: 99%

Production, purification, characterization, and applications of lipases

Sharma

Chisti

Banerjee

2001

Biotechnology Advances

1,255

712

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“…In the present investigation, statistical design of experiments (DoE) was used which is generally employed for obtaining the largest possible amount of information about a system with the smallest number of experiments [33]. The classical method of chemical synthesis optimization involves varying one parameter at a time by keeping others constant; however this method is ineffective, as it fails to understand relationships between the variables and the responses [34,35]. By using DoE, degree of substitution (DS) was optimized in synthesis of N-carboxybutylchitosan and 1-allyloxy-2-hydroxy-propylstarch previously [36,37].…”

Section: Determination Of Degree Of Substitution (Ds)mentioning

confidence: 99%

Development of novel thiolated carboxymethyl-gellan gum as potential mucoadhesive polymer: Application of DoE

More¹,

Bhamare²,

Bhavsar³

et al. 2017

Adv Mater Sci

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The objective of the present investigation was to synthesize soluble carboxymethyl-gellan gum (CMG) from gellan gum (GG) using statistical experimental design (DoE) strategy. Experimental design can potentially calculate the optimum reaction condition required to obtain response i.e. degree of substitution (DS) of CMG, which was statistically assessed. The effect of two process factors, namely concentration of monochoroacetic acid and water:isopropanol ratio on DS was investigated. Another objective of present study was to improve the mucoadhesive strength of optimized CMG by its covalent attachment with thiol moieties so as to get thiolated carboxymethyl-gellan gum (TCG). Synthesized CMG and TCG were characterized by FT-IR, DSC and NMR. Polymer compacts of TCG exhibited 3.73 fold greater adhesive strength on goat intestinal mucosa as compared with bare GG. In accordance with the promising results obtained; synthesized TCG can be considered as potential modified polymer for further drug delivery applications, especially mucoadhesive systems.

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“…Lipases are an important group of biotechnologically relevant enzymes, and properties such as their ability to perform specific biotransformations including esterification and transesterification and their stability have made them increasingly popular in the food, pharmaceutical, detergent, cosmetic and oleochemical industries (Park et al 2005;Grbavcic et al 2007;Gupta et al 2007). For many industrially important biotransformations, biotechnological interest in these enzymes has increased owing to these distinctive features (Chowdary et al 2001;Hamsaveni et al 2001;Krishna et al 2001;Rao and Divakar 2001;Zhang et al 2001;Castro et al 2004;Carvalho et al 2005;Fucinos et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Thermostable lipase from novelPseudomonassp. VITSDVM1 isolated from bovine milk

Devi

Mohanasrinivasan

Chetna

et al. 2015

Frontiers in Life Science

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The main aim of this study was to identify lipase-producing bacteria from bovine milk samples. The strain possessing the highest lipase activity was identified by physiological and molecular characterization. 16S rRNA phylogenetic analysis showed highest similarity with Pseudomonas sp. and the strain was named Pseudomonas sp. VITSDVM1. The lipase productivity was improved by varying the utilization of substrates, i.e. banana and potato peel extracts, in the production medium. The results of optimization studies showed that lipase activity was maximum with groundnut oil, compared with the other carbon sources used, and with peptone as a nitrogen source. The optimum pH and temperature were 8 and 40°C, respectively. The optimum substrate concentration was 20% for the incubation period of 72 h with a maximum activity of 4.66 U mL −1 . The purified lipase enzyme analyzed by high-performance liquid chromatography revealed a retention time of 3.6 min. Hence, lipase production using agricultural waste such as banana and potato peels could be used in industrial applications.

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Response surface methodological approach for the synthesis of isobutyl isobutyrate

Cited by 139 publications

References 9 publications

Production, purification, characterization, and applications of lipases

Production, purification, characterization, and applications of lipases

Development of novel thiolated carboxymethyl-gellan gum as potential mucoadhesive polymer: Application of DoE

Thermostable lipase from novelPseudomonassp. VITSDVM1 isolated from bovine milk

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