Response regulator ArcA of Salmonella enterica serovar Typhimurium downregulates expression of OmpD, a porin facilitating uptake of hydrogen peroxide

Calderón, Iván L.; Morales, Eduardo; Nelson, Jared L.; Chahúan, Catalina A.; Collao, Bernardo; Gil, Fernando; Villarreal, José Miguel; Ipinza, Francisco; Mora, Gabriela; Saavedra, Claudia P.

doi:10.1016/j.resmic.2010.11.001

Cited by 27 publications

(40 citation statements)

References 52 publications

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“…During the last years, evidence has emerged from our laboratory that illustrate some roles for the porins. We previously demonstrated that OmpD and the minor porin OmpW facilitate the uptake of hydrogen peroxide (H 2 O 2 ) and/or hypochlorous acid (HOCl) [6], [7], two oxidizing molecules produced in the phagocytic oxidative burst. Porins may also act as virulence factors.…”

Section: Introductionmentioning

confidence: 99%

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Participation of the Salmonella OmpD Porin in the Infection of RAW264.7 Macrophages and BALB/c Mice

et al. 2014

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Salmonella Typhimurium is the etiological agent of gastroenteritis in humans and enteric fever in mice. Inside these hosts, Salmonella must overcome hostile conditions to develop a successful infection, a process in which the levels of porins may be critical. Herein, the role of the Salmonella Typhimurium porin OmpD in the infection process was assessed for adherence, invasion and proliferation in RAW264.7 mouse macrophages and in BALB/c mice. In cultured macrophages, a ΔompD strain exhibited increased invasion and proliferation phenotypes as compared to its parental strain. In contrast, overexpression of ompD caused a reduction in bacterial proliferation but did not affect adherence or invasion. In the murine model, the ΔompD strain showed increased ability to survive and replicate in target organs of infection. The ompD transcript levels showed a down-regulation when Salmonella resided within cultured macrophages and when it colonized target organs in infected mice. Additionally, cultured macrophages infected with the ΔompD strain produced lower levels of reactive oxygen species, suggesting that down-regulation of ompD could favor replication of Salmonella inside macrophages and the subsequent systemic dissemination, by limiting the reactive oxygen species response of the host.

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Section: Introductionmentioning

confidence: 99%

“…Typhi genome, which could be related to the particular characteristics of the S . Typhimurium infective cycle [4], [7], [16], [17]. Regarding the role of OmpD in virulence, Dorman et al .…”

Section: Introductionmentioning

confidence: 99%

Participation of the Salmonella OmpD Porin in the Infection of RAW264.7 Macrophages and BALB/c Mice

et al. 2014

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“…Under H 2 O 2 and HOCl stress in vitro , S . Typhimurium ArcA downregulates genes encoding OmpC, OmpD, OmpF, and OmpW porins [28, 29]. Inside neutrophils, however, bacteria through ArcA upregulate ompD , ompF , and ompW (Fig 5), while in macrophages ArcA downregulates ompD and ompF and upregulates ompW (S7 Fig).…”

Section: Resultsmentioning

confidence: 99%

“…Typhimurium porins (OmpD, OmpW, OmpC, OmpS2, and OmpF) mediate H 2 O 2 and HOCl influx. Expression of these porins is also down-regulated in response to H 2 O 2 and HOCl, and their inactivation increases Salmonella ROS resistance [28, 29]. Accordingly, the absence of OmpD increases S .…”

Section: Introductionmentioning

confidence: 99%

“…Typhimurium invasion and proliferation in RAW 264.7 macrophages, as well as proliferation and systemic dissemination in BALB/c mice [30]. We have previously ruled out a role for SoxS and OxyR in the negative regulation of porin genes; instead, the expression of several of these porins was regulated by ArcA [28, 29], a transcription factor in the ArcAB two-component system. ArcAB, which comprises the ArcA response regulator and the sensor histidine kinase ArcB, responds to oxygen [31–33].…”

Section: Introductionmentioning

confidence: 99%

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The ArcAB two-component regulatory system promotes resistance to reactive oxygen species and systemic infection by Salmonella Typhimurium

et al. 2018

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Salmonella enterica Serovar Typhimurium (S. Typhimurium) is an intracellular bacterium that overcomes host immune system barriers for successful infection. The bacterium colonizes the proximal small intestine, penetrates the epithelial layer, and is engulfed by macrophages and neutrophils. Intracellularly, S. Typhimurium encounters highly toxic reactive oxygen species including hydrogen peroxide and hypochlorous acid. The molecular mechanisms of Salmonella resistance to intracellular oxidative stress is not completely understood. The ArcAB two-component system is a global regulatory system that responds to oxygen. In this work, we show that the ArcA response regulator participates in Salmonella adaptation to changing oxygen levels and is also involved in promoting intracellular survival in macrophages and neutrophils, enabling S. Typhimurium to successfully establish a systemic infection.

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The Role of Alternative Sigma Factors in Pathogen Virulence

Roberts

Rowley

Kormanec

et al. 2017

Foodborne Pathogens

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Alternative sigma factors enable bacteria to change the promoter specificity of the core RNA polymerase to enable the expression of genes that give them advantages in particular situations. The number of alternative sigma factors that bacteria produce varies greatly. Some bacteria, particularly those that reside in the soil have genes for multiple sigma factors. The soil living gram positive bacteria Sorangium cellulosum currently holds the record for the number of sigma factor genes at 109. Alternative sigma factors play important roles in the life cycle of many foodborne bacterial pathogens. In this review we will discuss: the structure and function of alternative sigma factors; the different families of alternative sigma factors; their regulation; the role of particular alternative sigma factors and the genes they control in the biology (particularly pathogenesis) of foodborne bacterial pathogens

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Response regulator ArcA of Salmonella enterica serovar Typhimurium downregulates expression of OmpD, a porin facilitating uptake of hydrogen peroxide

Cited by 27 publications

References 52 publications

Participation of the Salmonella OmpD Porin in the Infection of RAW264.7 Macrophages and BALB/c Mice

Participation of the Salmonella OmpD Porin in the Infection of RAW264.7 Macrophages and BALB/c Mice

The ArcAB two-component regulatory system promotes resistance to reactive oxygen species and systemic infection by Salmonella Typhimurium

The Role of Alternative Sigma Factors in Pathogen Virulence

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